非生物胁迫条件下10个玉米ZmDOF基因表达模式分析

doi:10.7668/hbnxb.20191701

摘要/Abstract

摘要： 为了研究玉米转录因子Dof（DNA-binding with one zinc finger）锌指蛋白在玉米生长发育及非生物逆境胁迫响应途径中的作用与生物学功能。利用qRT-PCR技术系统研究了10个ZmDOF基因在玉米不同组织中以及应答不同非生物逆境胁迫下响应表达模式。进化树以及基因结构分析结果表明，这10个ZmDOF基因分布在3个不同的亚家族。qRT-PCR分析结果显示，这10个基因具有组织表达特异性。7个基因主要在雄花中表达，2个基因主要在授粉15 d后的小穗中表达，1个基因主要在根中表达，表明这10个基因可能参与这些组织的生长发育进程。在盐或干旱胁迫处理下，大部分基因的表达模式都发生了明显的改变，预示着这些基因参与玉米幼苗应答盐或干旱胁迫响应信号途径。在铵态氮缺乏胁迫下，ZmDOF3的表达上调了5倍以上。在硝态氮缺乏胁迫下，ZmDOF23和ZmDOF46的表达量下降了90%以上，表明这些基因可能参与调控玉米幼苗氮代谢平衡或者信号转导途径。

关键词: 玉米, DOF转录因子, 非生物胁迫, 进化树, 组织表达

Abstract: In order to study the role and function of DNA-binding with one finger (DOF) proteins in maize growth and development, the expression profiles of 10 ZmDOFs genes in different tissues or in response to various abiotic stresses have been carefully studied using qRT-PCR. The result of the phylogenetic tree and gene structure analysis showed that 10 ZmDOFs divided into three subclasses. The result of qRT-PCR revealed that 10 ZmDOFs exhibited obvious expression preference or specificity in different organs, 7 ZmDOFs high expression in tassels, 2 ZmDOFs in young embryo after pollination 15 days and 1 ZmDOF in root, indicating the important roles in the processes of maize growth and development. Results by qRT-PCR revealed the altered expression pattern of most of ZmDOFs in response to salt or osmotic stresses, indicating these gene involved in the regulation of salt or osmotic stress response pathway. ZmDOF3 was up-regulated by at least 5-fold under NH₄⁺ depletion treatment, while ZmDOF23 and ZmDOF46 reduced by 90% under NO₃^- depletion treatment, indicated these genes were involved in nitrogen homeostasis or signaling pathway.

Key words: Maize, DOF transcription factor, Abiotic stress, Phylogenetic tree, Tissue expression

中图分类号:

S513.03
Q78

贾利强, 赵秋芳, 陈曙. 非生物胁迫条件下10个玉米ZmDOF基因表达模式分析[J]. 华北农学报, 2021, 36(2): 19-27. doi: 10.7668/hbnxb.20191701.

JIA Liqiang, ZHAO Qiufang, CHEN Shu. Expression Analysis of 10 ZmDOFs under Abiotic Stress in Maize[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 19-27. doi: 10.7668/hbnxb.20191701.

http://www.hbnxb.net/CN/Y2021/V36/I2/19

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