摘要： 对72个大豆品种苗期耐低钾特性进行鉴定,并筛选耐低钾种质资源,利用获得的不同类型大豆材料研究耐低钾特性与体内保护酶系统的关系。结果表明,在5个钾不同处理浓度下,0.01 mmol/L适宜作胁迫浓度,2.50mmol/L适宜作对照浓度;以植株生物量相对值为筛选指标,可鉴定供试品种的耐低钾特性。研究发现,供试的大豆品种耐低钾能力存在较大变异,筛选出5个耐低钾特性较强的品种。另外,对不同耐低钾类型品种的叶片保护酶系统分析发现,耐低钾特性与胞内保护酶系统活性存在一定联系。

关键词: 大豆, 种质资源, 低钾胁迫, 筛选指标, 品种鉴定

Abstract: In order to evaluate the soybean genotypes tolerant to low potassium(K) stress,and to screen the high tolerant germplasms,seventy-two varieties from Hebei soybean-growing areas were identified with different potassium concentrations at seedling stage.The results showed that the 0.01 mmol/L could be the concentration for potassium-deficiency determination,and the 2.50 mmol/L for potassium-normal concentration in supplied solution.The relative values,ratios of weight in low K and weight in adequate K of shoots or roots,could be used as the screening indices.There were significant difference among 72 varieties in two different screening regimes(0.01,2.50 mmol/L).According to the relative indices of shoot and root weights,five soybean varieties were screened out for their high tolerance to low potassium stress.In addition,the protective enzymes of different potassium tolerant varieties showed that the difference of potassium nutrient effective types can be expressed by the protective enzyme systems.The screening results of soybean germplasms and the relationship between low-K tolerance and protective enzymes can be used to clone more K-efficiency genes,study the mechanisms of plant high K-efficiency and breed high tolerant varieties to low K stress.

Key words: Soybean, Germplasm resources, Low potassium stress, Screening indices, Variety identification

中图分类号: 

• S565.1