摘要： 采用系列稀释法和平板涂布法从黑龙江漠河地区的永久冻土层20份土壤样品中分离得到172株细菌菌株,经平板对峙法初筛得到10株拮抗菌,经复筛得到了1株对甘蓝枯萎病菌有较强抑制作用的拮抗细菌MH71菌株。通过菌落形态观察、生理生化测定、16S rDNA和16S~23S rDNA之间的ITS特异性序列分析等,表明MH71菌株为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。该菌株对镰刀病菌、灰葡萄孢及炭疽病菌等15种农业生产上常见的植物病原真菌和黄瓜角斑、平菇褐斑、大白菜黑腐和啤酒酵母菌等植物病原细菌和酵母菌均有不同程度的拮抗作用,表现出广谱抗菌活性。此结果表明:MH71菌株是一株极具开发潜力的生防菌株。
关键词:
甘蓝枯萎病,
拮抗细菌,
解淀粉芽孢杆菌,
永久冻土层
Abstract: 172 strains of bacteria were isolated from 20 soil samples collected from the permafrost of Mohe area,Heilongjiang Province,by the serial dilution and spread-plate methods. Plate stand-opposite culture method for preliminary screening of 10 antagonistic strains and one bacterial strain MH71,exhibited an extensive antagonistic effect on Fusarium oxysporum f. sp. conglutinans. The strain was identified as Bacillus amyloliquefaciens based on morphological observation,physiological and biochemical characteristics,16S rDNA sequence homology and 16S-23S ITS specific PCR detection. Moreover,the strain showed broad-spectrum antimicrobial activities,such as Fusarium spp.,Botrytis cinerea,Colletotrichum sp.,15 plant pathogenic fungi and Pseudomonas syringae pv. lachrymans,P. tollasii and Xanthomonas campestris pv. campsetris plant pathogenic bacteria and Saccharomyces cerevisiae. Results showed that strain MH71 had a great potential on bio-control application.
Key words:
Fusarium oxysporum f. sp. conglutanans,
Antagonistic bacteria,
Bacallus amyLoLaquefacaeus,
Permafrost
中图分类号:
卢彩鸽, 张殿朋, 刘伟成, 吴慧玲, 刘霆, 刘德文. 一株甘蓝枯萎病拮抗细菌的筛选、鉴定及其抑菌活性测定[J]. 华北农学报, 2014, 29(1): 195-202. doi: 10.7668/hbnxb.2014.01.035.
LU Cai-ge, ZHANG Dian-peng, LIU Wei-cheng, WU Hui-ling, LIU Ting, LIU De-wen. Screening,Identification and Activity Analysis of Antagonistic Bacteria against Cabbage Wilt Disease[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 195-202. doi: 10.7668/hbnxb.2014.01.035.