大白菜抗根肿病基因定位及连锁标记挖掘

doi:10.7668/hbnxb.201751417

摘要/Abstract

摘要： 为了明确抗根肿病大白菜材料G6所含抗病基因，开发连锁标记，以高感根肿病的大白菜高代自交系G4、高抗根肿病的大白菜高代自交系G6、G4和G6杂交得到的F₁及F₁自交构建的F₂分离群体为材料，通过人工接种、表型鉴定和遗传学分析，发现该抗病材料中的根肿病抗性由显性单基因控制。进一步扩大F₂群体数量对根肿病抗性基因的初定位，筛选与其连锁的分子标记，利用JoinMap 4.0软件对多态性标记进行连锁分析，获得了5个与大白菜抗根肿病基因连锁的InDel标记，其中最近的两侧标记为BrID10727和BrID10867，与抗病基因的遗传距离分别为4.6，2.5 cM，抗病基因定位在大白菜染色体A08上。此外，经验证发现基于Crr1基因序列新开发的多态性标记BrID10381不在新定位抗根肿基因初定位区间内，因此，可以推断新定位抗根肿基因位点与Crr1并非同一位点，且标记BrID10381可以用于Crr1基因的分子标记辅助选择。

关键词: 大白菜, 根肿病, 基因定位, 连锁标记

Abstract: In order to identify the clubroot resistance gene of Chinese cabbage in G6 and develop linkage markers, high-generation inbred line G4 of Chinese cabbage with high-susceptible to clubroot disease, high-generation inbred line G6 of Chinese cabbage with high-resistant to clubroot disease, F₁ obtained by crossing G4 and G6, and the F₂ generation separation populations constructed by F₁ selfing were used as materials. By artificial inoculation, phenotypic identification and genetic analysis, it was found that clubroot disease resistance in the disease resistance materials were controlled by a dominant single gene. Further expanding the number of F₂ generation populations to map the genes of clubroot disease and screening molecular markers linked to disease resistance genes, the linkage analysis of polymorphic markers was performed using JoinMap 4.0 software, and five InDel markers linked to the resistance gene of Chinese cabbage were obtained. The most recent markers on both sides were BrID10727 and BrID10867, the genetic distance from the disease resistance genes were 4.6,2.5 cM, respectively, and the disease resistance gene was located on the chromosome A08 of Chinese cabbage. In addition, it was found that the newly developed polymorphic marker BrID10381 based on the Crr1 gene sequence was located outside the initial localization range of the newly located clubroot resistance gene, so it could be inferred that the newly located clubroot resistance gene locus was not the same locus as Crr1, and the marker BrID10381 could be used for molecular marker assisted selection of Crr1 gene.

Key words: Chinese cabbage, Clubroot disease, Gene mapping, Linkage marker

中图分类号:

S634.1

王超楠, 张斌, 张红, 温娟娟, 王涛. 大白菜抗根肿病基因定位及连锁标记挖掘[J]. 华北农学报, 2019, 34(3): 203-208. doi: 10.7668/hbnxb.201751417.

WANG Chaonan, ZHANG Bin, ZHANG Hong, WEN Juanjuan, WANG Tao. Mapping of Clubroot Resistance Gene and Developing Linkage Markers in Brassica rapa L. ssp. pekinensis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(3): 203-208. doi: 10.7668/hbnxb.201751417.

http://www.hbnxb.net/CN/Y2019/V34/I3/203

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