利用抗、染根肿病F2群体构建大白菜AFLP遗传连锁图谱

doi:10.7668/hbnxb.2009.02.014

华北农学报 ›› 2009, Vol. 24 ›› Issue (2): 64-70. doi: 10.7668/hbnxb.2009.02.014

所属专题： 白菜；

利用抗、染根肿病F2群体构建大白菜AFLP遗传连锁图谱

王淼¹, 王剑¹, 李宏博¹, 林荣杓², 朴钟云¹

1. 沈阳农业大学园艺学院, 辽宁沈阳 110161;
2. 忠南大学农业生命科学学院, 园艺系, 韩国大田 305764

收稿日期:2008-12-10 出版日期:2009-04-28
通讯作者: 朴钟云( 1966- ), 男, 吉林人, 教授, 博士, 主要从事蔬菜生物技术。
作者简介:王淼( 1982- ), 女, 辽宁沈阳人, 在读硕士, 主要从事利用分子标记辅助选择选育抗根肿病大白菜方面的研究.
基金资助:
国家自然科学基金(30771468);国家"863"项目(2006AA100108)

Constructed an AFLP Genetic Linkage Map of Chinese Cabbage with Resistant and Susceptible F2 Lines

WANG Miao¹, WANG Jian¹, LI Hong-bo¹, LIM Yong-pyo², PIAO Zhong-yun¹

1. College of Horticulture,Shenyang Agricultural University, Shenyang 110161, China;
2. Department of Horticulture, College of Agriculture and Life Science, Chungnam National University, Daejeon 305764, Korea

Received:2008-12-10 Published:2009-04-28

摘要/Abstract

摘要： 以易感染根肿病的大白菜(Brassica campestris ssp.pekinensis)自交系94SK和抗根肿病的CR Shinki DH系为亲本,通过杂交获得F1,并选择一株F1植株进行自交,构建了F2作图群体.利用扩增出与根肿病抗性基因CRb连锁标记的1对AFLP引物组合Pst+GNN/Mse+CNN构建大白菜遗传连锁图谱.这些AFLP引物组合共扩增出322个亲本间表现出多态性的AFLP标记.应用其中6个与CRb基因紧密连锁的AFLP标记转化成的SCAR标记和清晰可见的大于100 bp的211个AFLP标记构建了一张包含19个标记位点、10个连锁群、覆盖长度为56 cM,平均图距3.3 cM的遗传图谱.抗根肿病基因CRb定位在第一条连锁群9 cM的范围内.研究表明,利用PstI/MseⅠ和EcoRⅠ/MseⅠ引物组合以及其他分子标记类型构建遗传连锁图谱,并有效减少遗传图谱中聚集和间隙现象的产生提供了一定的科学依据.同时,该图谱的构建对在基因组范围内通过分子标记辅助选择选育大白菜根肿病抗性品种奠定了基础.

关键词: 大白菜, AFLP, 遗传连锁图谱, 根肿病

Abstract: Clubroot disease,caused by Plasmodiophora brassicase War. is soil- born,obligate plant pathogen that causes the development of galls on root tissue in Crucifer.The clubroot is one of the most damaging diseases of brassica crops and is the most serious disease of cruciferous crops in the temperate zone.An F2mapping population was developed from a cross between Chinese cabbage inbred line of 94 SK,a line suscept ible to clubroot disease,and CR Shinki DHline,a line resistance to clubroot disease.Seventeen Pst+ GNN/Mse+ CNN primer combinations generating AFLP markers linked to clubroot resistance gene CRb were employed to construct a linkage map of Chinese cabbage.Of 322 polymorphic bands observed between two parental lines, 211 AFLP and 6 SCRA marker conversed from AFLP marker linked to CRb were used for construction of linkage map.Most of polymorphic bands displayed normal segregat ion pattern in an F2 population. Only 4 markers were seriously distorted. Among 217 markers, 179 were mapped to ten linkage groups at LOD value of 4. 3 using JoinMap version 3.The linkage map developed here covers a total map distance of 576 cM corresponding 3.3 cM per marker.The clubroot resistance gene CRb was mapped to the interval of 9 cM in linkage group 1.This result indicated PstI/MseⅠ和EcoRⅠ/MseⅠ primer combinations constructed linkage map with other types makers, and provided a certain amount of scientific evidence to effectively reduce the accumulation of genet ic maps and the generation gap.The construction of the map was the basic of breeding Chinese cabbage resistant to clubroot disease through the molecular marker- assisted selection in the range of the genome.

Key words: Chinese cabbage, AFLP, Genetic linkage map, Clubroot disease

中图分类号:

S634.03

王淼, 王剑, 李宏博, 林荣杓, 朴钟云. 利用抗、染根肿病F2群体构建大白菜AFLP遗传连锁图谱[J]. 华北农学报, 2009, 24(2): 64-70. doi: 10.7668/hbnxb.2009.02.014.

WANG Miao, WANG Jian, LI Hong-bo, LIM Yong-pyo, PIAO Zhong-yun. Constructed an AFLP Genetic Linkage Map of Chinese Cabbage with Resistant and Susceptible F2 Lines[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(2): 64-70. doi: 10.7668/hbnxb.2009.02.014.

http://www.hbnxb.net/CN/Y2009/V24/I2/64

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利用抗、染根肿病F2群体构建大白菜AFLP遗传连锁图谱

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