转基因棉对棉蚜生长发育及其主要代谢物质含量的影响

doi:10.7668/hbnxb.2011.04.020

摘要/Abstract

摘要： 近年来,转基因棉田非靶标害虫种群数量呈明显的上升趋势,棉蚜是转Bt(Bacillus thuringiensis)基因棉田发生危害较严重的非靶标刺吸性害虫之一。在实验室内,棉蚜分别用转Bt基因棉和亲本常规棉饲养40代以上,采用酶联免疫法(ELISA)检测转Bt基因棉SGK321上棉蚜体内的Bt蛋白含量,研究转Bt基因棉对棉蚜生长发育和体内3种主要代谢物质脂类、蛋白质和糖含量的影响。研究结果表明,长期饲养于转Bt基因棉上的棉蚜体内能检测到痕量的Bt蛋白。与亲本常规棉相比,转Bt基因棉上棉蚜寿命延长,产蚜数减少,但差异不显著。生命表参数也无显著差异。转Bt基因棉上的棉蚜体内总蛋白含量、三油酸甘油酯含量增加,总糖含量减少,但与亲本常规棉相比差异不显著。初步结果表明从长期效应看,SGK321对棉蚜的生长发育、主要代谢物质含量均无显著影响。

关键词: 转Bt基因棉, 非靶标害虫, 棉蚜, 代谢物质, ELISA

Abstract: For the breeding of drought resistant,low water consumption,high water use efficiency of varieties,the water use efficiency(WUE) of leaf can affect the yield of crop directly.In order to determine the relationship between WUE and genetics background,and to elucidate the mechanism of that how WUE affect the yield,22 maize inbred lines were grown under water controlled condition.the following physiology index,water use efficiency of inbred lines leaf(WUE),photosynthetic rate(Pn),transpiration rate(Tr),stomatal conductance(Gs),intercellular CO₂ concentration(Ci),the relative values of chlorophyll(SPAD) and fluorescence parameters of inbred lines leaf,were assayed.The results showed that there was significant difference in the WUE among different inbred lines.And significant difference in the degree of sensitivity to water among these inbred lines was also observed;Gs,Pn,Tr declined in moderate water stress,among the decrease of Tr was greater than the decrease of Pn,so that the WUE was increased than in the normal irrigation treatments;the relation of WUE of different inbred lines leaf and Pn,Fm,Fv,Fv/Fm and Fv/Fo was significant correlation.

Key words: Transgenic Bt cotton, Non-target pest, Aphis gossypii, Metabolite, ELISA

中图分类号:

S562． 01

薛丽, 肖达, 卢延, 宋敦伦, 高希武. 转基因棉对棉蚜生长发育及其主要代谢物质含量的影响[J]. 华北农学报, 2011, 26(4): 115-120. doi: 10.7668/hbnxb.2011.04.020.

XUE Li, XIAO Da, LU Yan, SONG Dun-lun, GAO Xi-wu. Water Use Efficiency of Maize Inbred Line Leaf and the Physiological Changes[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(4): 115-120. doi: 10.7668/hbnxb.2011.04.020.

http://www.hbnxb.net/CN/Y2011/V26/I4/115

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