摘要： 蚜虫作为病毒的传播介体为害植物造成的经济损失远远超过蚜虫本身,因此急需建立快速、准确、灵敏的蚜虫带毒检测方法。根据百合无症病毒(LSV)和烟草花叶病毒(TMV)外壳蛋白基因保守序列设计引物与探针,制备基因芯片;用Cy3标记下游引物,提取饲毒(LSV和TMV)棉蚜、桃蚜总RNA,RT-PCR扩增产物与芯片杂交,荧光扫描仪检测并分析信号。结果表明,该芯片可以同时检测上述2种蚜虫的带毒情况,此方法有较好的特异性和重复性,能快速、准确地对介体蚜虫的带毒情况进行检测。

关键词: 棉蚜, 桃蚜, 百合无症病毒, 烟草花叶病毒, 基因芯片, 检测

Abstract: Aphid transmission of the virus as a mediator harm plants far more than economic losses caused by aphids themselves. So,rapid,sensitive and specific method,by which viruses carried by aphids can be detected simultaneously,is needed urgently. In this study,according to the Lily symptomless virus(LSV) and the Tobacco mosaic virus(TMV) coat protein gene sequence,primers and probes were designed,gene chip was prepared. The downstream primers were labeled by Cy3. The total RNA was extracted from Aphis gossypii and Myzus perdicae raised with lily infected by LSV and TMV respectively. The product amplified by RT-PCR using the labeled primers was hybridized with the gene chip. The hybridization signal was detected and analyzed by a fluorescence scanner. The result showed that the chip can simultaneously detect both aphids infected cases,this method has good specificity and reproducibility,fast and accurate on the mediator infected aphids were detected.

Key words: Aphais gossypii, Myzus perdicae, Lily symptomless virus, Tobacco mosaic virus, Gene, Detection

中图分类号: 

• S432.4

• Q78