6-苄基腺嘌呤延缓水稻衰亡效应的蛋白质组学分析

doi:10.7668/hbnxb.2014.01.003

摘要/Abstract

摘要： 以苗期杂交水稻威优916为试验材料,分别以蒸馏水和8 mg/L 6-苄基腺嘌呤溶液培养,通过对功能叶片蛋白质组差异表达分析,以揭示养分胁迫下6-苄基腺嘌呤延缓水稻衰亡的效应及其机理。叶片全蛋白质经双向电泳分离,获得了22个响应6-苄基腺嘌呤处理的蛋白质,经质谱分析,其中15个功能得到鉴定,涉及光合作用、呼吸作用、激素代谢及逆境反应等多个代谢途径。分析结果显示,养分胁迫下,6-BA诱导了叶片中光合蛋白质、呼吸代谢相关蛋白质的表达量上调,减少衰亡发生密切相关蛋白质的积累,从而提高叶片光合能力,促进呼吸,满足植株生长对物质及能量的需求,延缓植株衰亡。

关键词: 水稻, 6-苄基腺嘌呤, 衰亡, 蛋白质组学

Abstract: In order to reveal the mechanism of 6-benzyl adenine deferring contabescence of rice,the hybrid rice Weiyou 916 seedlings were cultivated with distilled water and 6-benzyl adenine(8 mg / L),respectively,and the proteins expression profile of functional leaves were investigated by using the approach of proteomics. Proteins were extracted from rice functional leaf and separated by two-dimensional gel electrophoresis,then 22 differential expression proteins that induced by 6-benzyl adenine were obtained,among which 15 proteins were identified by matrix-assisted laser desorption / ionization-time of flight mass spectrometry,and categorized into 4 groups of functions including photosynthesis,respiration,hormone metabolism and stress response. Results showed that under nutrient stress condition,6-benzyl adenine had benifical effect on rice plant: the expression abundance of proteins associated with photosynthesis and respiration increased,and proteins associated with contabescence were down-regulated in expression abundance. Thereby,application of 6-benzyl adenine chould enhanced the photosynthesis and respiration directly, provided the material and energy for plant growth and defered rice contabescence.

Key words: Rice, 6-benzyl adenine, Contabescence, Proteomics

中图分类号:

S312.03
Q78

邵彩虹, 唐秀英, 李明心, 李瑶, 王萍, 陈金, 谢金水. 6-苄基腺嘌呤延缓水稻衰亡效应的蛋白质组学分析[J]. 华北农学报, 2014, 29(1): 14-19. doi: 10.7668/hbnxb.2014.01.003.

SHAO Cai-hong, TANG Xiu-ying, LI Ming-xin, LI Yao, WANG Ping, CHEN Jin, XIE Jin-shui. Proteomics Analysis of Mechanism of 6-benzyl Adenine Deferring Contabescence of Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 14-19. doi: 10.7668/hbnxb.2014.01.003.

http://www.hbnxb.net/CN/Y2014/V29/I1/14

参考文献

1 吴岳轩，吴振球丁酸对杂交水稻根系代谢活性及叶片衰老进私I的影响[J]杂交水稻，1991(4);29-34
2 王少先，彭克勤，萧浪涛双氰氨对水稻根系及光合特性和经济性状的影响[J]湖南农业大学学报:自然科学版，2003,29(1);18-21
3 王丰，程方民植物激素与水稻产量的关系及其在生产上的应用[J]现代化农业, 2003 (10); 20-21
4 陶龙兴，王熹，黄效林，等植物生长调节剂在农业中的应用及发展趋势[J]浙江农业学报，2001,13(5);322-326
5 黄静静，王绍华，李刚华，等6-节基腺嘌吟对水稻颖花分化影响机制的研究[J]南京农业大学学报，2009,32(3):8-13
6 杨知建，徐庆国，朱春生，等6-BA处理对水稻根系中后期生长的影响[J]湖南农业大学学报，2009,35(5):462-465
7 蔡永萍，陈静娴，聂凡水稻抽穗期喷施6-BA, ABA对旗叶衰老及同化物运输的影响[J]安徽农业科学，1996,24(2):113-1158 杨安中，牟筱玲，李孟良，等喷施细胞分裂素类物质对地膜旱作水稻防衰及增产效应[J]水土保持学报，2008，19(2):199-200
9 郭彦，张文会，巍秀俭，等6-BA对干旱胁迫条件下水稻芽期生长的影响[J]安徽农业科学，2005,33 (9):1992-1993
10 梁建秋，梁颖几种生长物质对油菜幼苗抗湿性的影响[J]西南师范大学学报，2009,34(1);s8-6211 徐皓6-BA对蚕豆离体叶片衰老的延缓作用[J] 江苏农业科学，2008(4);49-51
12 Wang X C，Li X F,Li Y X. A modified coomassie bril-liant blue staining method at nanogram sensitivity com-patible with proteomic analysis[J]Biotechnology Let-ters,2007,29:1999-1603
13 夏其昌，曾嵘蛋自质化学与蛋自质组学[M]北京:科学出版社，2004: 278
14 谢金水，邵彩虹，唐秀英，等养分胁迫对籽粒灌浆期水稻叶片衰老影响的蛋自质组学分析[J]中国水稻科学，2011,28(2);143-149
15 Hajduch M，Rakwal R,Garawal G K,et al. High-resolu-tion two-dimensional elecarophoresis separation of pro-teins from metal-stressed rice (Oryza. native L)leaves: Drastic: reducaions/fragmentation of ribulose-1,s-bispho-tosphate carboxylase/oxygenase and inducaion of stress-related proteins[J]Elecarophoresis，2001，22(13): 2824-2831
16 瞿礼嘉植物生物化学与分子生物学[M]北京:科学出版社，2004 } 131-483
17 Wollman F A, Minai L, Nechushtai R. The biogenesis and assembly of photosynt hetic proteins in thylakoid membranesl[J]Biochim Biophys Acta, 1999，1411 (1);21-85
18 Friso G, Giacomelli L, Ytterberg A J, et al. In depth a-nalysis of the thylakoid membrane proteome of Arabidop-si,s thalia}aa chloroplasts new proteins, new funcaions,and a plastid proteome database[J]Plant Cell, 2004，16 (2);478-499
19 Lea P J, Millin B J. Alternative route for nitrogen in higher plants [J]. Nature,1974,251(5476); 614-616
20 Lothier J, Gaufichon L, Sormani R, et al. The cytosolic glutamine synthetase GLN1;2 plays a role in the control of plant b owth and ammonium homeostasis in Arabidop-si.s rosettes when nitrate supply is not limiting [J]. Jour-nal of Experimental Botany,2011,62(4):1375-1390
21 Hirel B，Benin P,Quillere I, et al. Towards a better un-derstanding of the genetic: and physiological basis for ni-trogen use elficiency in maize[J]Plant Physiology, 2001，125(3):1258-1270
22 Harrison J, Crescenzo M P, Sene 0, et al. Does lowering glutamine synthetase acaivity in nodules modify nitrogen metabolism and growth of Lotus. japouicu.s[J]PlantPhysiology,2003，133(1);253-262
23 Cren M, Hirel B. Glutamine synthetase in higher plants:regulation of gene and protein expression from the organ tothe ce11[J]. Plant Cell Physiolog,1999, 40(12):1187-1193
24 Jones R J,Schreiher B M N. Role and funcaion of cyto-kinin oxidases in plants[J]Plant Growth Regulation,1997,23(1-2):122-134
25 Werner T, Motyka V, Strnad M, et al. Regulation of plantgrowth by cytokinin [J]. Proceedings of the National A-cademy of Science of USA, 2001，98(18):10487-10492
26 王军，侯丙凯植物小分子化合物的糖基化与糖基转移酶[J]植物生理学通讯，2008, 44 (5); 997-1002
27 吴华，严定平，罗越华水稻磷酸醋酶2A基因蛋自的原核表达载体构建、表达和纯化[J]贵州科学，2009,27(2);45-49
28 龚宁萍，陈朝银耐热碱性磷酸醋酶的研究进展[J]药物生物技术，2004,11(3);207-210
29 Singla-Pareek S L,Reddy M K,Sopory S K. Genetic: en-gineering of the glyoxalase pathway in tohacco leads toenhanced salinity tolerance[J]. Proceedings of the Na-tional Academy of Science of USA, 2003，100(25):14672-14677
30 Collinge M，Boller T. Differential inducaion of two potatogenes，Stprx2 and StNAC，in response to infecaion byPhytophthora cafe.stana.s and to wounding[J]Plant Mo-lecular Biology,2001,46(5);521-529
31 Fujita M, Fujita Y, Maruyama K, et al. A dehydrationin-duced NAC protein, RD26，is involved in a novel ABA-dependent sue signaling pathway[J]Plant Journal,2004,39(6);863-876
32 Tran L S,Nakashima K,Sakuma Y,et al. Isolation andfuncaional analysis of Arabidop.si,s stress-inducible NACtranscription facaors that bind to a drought-responsivecis-element in the early responsive to dehydration stress1 promoter[J]The Plant Ce11,2004，16(9):2481-2498
33 Delessert C，Kazan K,Wikon IW，et al. The transcrip-tion facaor ATAF2 represses the expression of pathogene-sis-related genes in Arabidop.si,s[J].. Plant Journa1,2005，43(5);745-757
34 孙虎，薛保国，杨丽荣，等植物硫氧还蛋自系统[J]基因组学与应用生物学，2010, 29 (4); 748 -753
35 Kampranis S C，Damianova R, Atallah M, et al. A novelplant glutathione S-transferase/peroxidase suppressesBax lethality in yeast [J]. Journal of Biological Chemis-try,2000,275(38);29207-29216
36 王丽，王晓丽，刘仕，等植物草酸氧化酶及其基因的研究进展[J]中国农学通报，2010,26(7);48 -51

[1]	李霞, 罗丽卉, 周娅, 杨定清, 王棚, 李森. 秸秆还田对水稻-油菜轮作体系土壤活性有机碳组分及酶活性的影响[J]. 华北农学报, 2022, 37(5): 124-131.
[2]	王亚, 王越涛, 申关望, 王付华, 王生轩, 白涛, 尹海庆. *聚合R基因Pigm和非R基因bsr-d1改良水稻稻瘟病抗性*[J]. 华北农学报, 2022, 37(5): 157-165.
[3]	韩政宏, 段宇轩, 徐善斌, 王敬国, 刘化龙, 杨洛淼, 贾琰, 辛威, 郑洪亮, 邹德堂. *利用CRISPR/Cas9技术敲除GS3和GS9基因改良水稻粒型性状*[J]. 华北农学报, 2022, 37(2): 9-17.
[4]	周丽平, 赵秋, 张新建, 宁晓光, 袁亮, 李燕婷, 赵秉强. 新型增效复合肥料对水稻养分吸收和产量的影响[J]. 华北农学报, 2022, 37(2): 112-120.
[5]	梁玉刚, 周晶, 余政军, 李静怡, 黄璜, 赵正洪. 稻鸭共育对直播水稻田间杂草群落组成及多样性的影响[J]. 华北农学报, 2022, 37(2): 160-170.
[6]	刘俊峰, 李漪濛, 梁超, 周婵婵, 王术, 贾宝艳, 黄元财, 王岩, 王韵. 施氮方式与行距配置对水稻冠层结构及产量的影响[J]. 华北农学报, 2022, 37(1): 77-85.
[7]	静莉丽, 彭廷, 赵亚帆, 赵帅兵, 王童童, 李源, 程远, 杜彦修, 张静, 孙红正, 赵全志. 灌浆充实调节剂对大穗型水稻弱势籽粒灌浆的调控效应[J]. 华北农学报, 2022, 37(1): 86-94.
[8]	徐令旗, 郭晓红, 张佳柠, 赵洋, 李晓蕾, 刘绍峰, 崔致远, 安懿亮, 吕艳东. 不同有机肥对旱直播水稻品质的影响[J]. 华北农学报, 2022, 37(1): 137-146.
[9]	高素红, 葛长虹, 周国娜, 姚淑娟, 路常宽, 高宝嘉. 绿盲蝽取食诱导赤霞珠冬芽全蛋白双向电泳体系的建立与优化[J]. 华北农学报, 2022, 37(1): 181-187.
[10]	陈子琪, 王伟苹, 赵宏强, 王皓, 韩笑, 杨洛淼, 辛威, 刘化龙, 郑洪亮, 王敬国. 利用籼粳交RIL群体进行水稻发芽期与芽期耐冷性QTL分析[J]. 华北农学报, 2022, 37(1): 50-57.
[11]	董林林, 沈明星, 全坚宇, 闻育琴, 沈园, 陶玥玥, 施林林, 陆长婴. 基肥正位深施对直播水稻产量和化肥利用的影响[J]. 华北农学报, 2021, 36(S1): 222-230.
[12]	张鹏, 吴佩聪, 单颖, 邹刚华, 丁哲利, 钱永德, 赵凤亮. 秸秆还田对热带土壤-水稻种植系统N₂O排放的影响[J]. 华北农学报, 2021, 36(S1): 260-266.
[13]	魏吉平, 代航, 李振勇, 丁紫苏, 唐凌, 栾鑫, 柯善文, 张向前. *水稻叶早衰突变体osles3的基因定位及其生物信息学分析*[J]. 华北农学报, 2021, 36(6): 45-53.
[14]	隆艳喜, 罗雁茹, 竺正航, 廖芷依, 王兰. 四倍体水稻蛋白质含量和谷蛋白基因表达研究[J]. 华北农学报, 2021, 36(6): 78-87.
[15]	腾海艳, 徐丹. OsSP1基因编辑和过表达对水稻生长和干旱抗性的影响[J]. 华北农学报, 2021, 36(5): 1-9.

选择文件类型/文献管理软件名称

选择包含的内容