华北农学报 ›› 2020, Vol. 35 ›› Issue (S1): 338-341. doi: 10.7668/hbnxb.20191405

所属专题: 甜椒辣椒 生物技术

• 资源环境·植物保护 • 上一篇    下一篇

辣椒抗疫病基因分子标记的筛选及检测

孟雅宁1, 严立斌1,2, 张红肖1, 赵海涛3, 范妍芹1   

  1. 1. 河北省农林科学院 经济作物研究所, 河北 石家庄 050051;
    2. 河北省蔬菜工程技术研究中心, 河北 石家庄 050051;
    3. 河北省农产品加工促进中心, 河北 石家庄 050011
  • 收稿日期:2020-05-21 出版日期:2020-12-28
  • 通讯作者: 范妍芹(1961-),女,山西屯留人,研究员,主要从事甜辣椒遗传育种研究。
  • 作者简介:孟雅宁(1984-),女,河北石家庄人,副研究员,硕士,主要从事蔬菜生物技术与遗传育种研究。
  • 基金资助:
    河北省重点研发计划项目(20326326D);河北省现代农业产业技术体系项目(HBCT2018030204);河北创新工程项目(2019-3-1-4);国家重点研发计划(2016YFD0101704)

Screening and Detection of Resistance to Phytophthora Blight Gene in Pepper Using Molecular Markers

MENG Yaning1, YAN Libin1,2, ZHANG Hongxiao1, ZHAO Haitao3, FAN Yanqin1   

  1. 1. Institute of Economic Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China;
    2. Hebei Province Engineering Research Center for Vegetables, Shijiazhuang 050051, China;
    3. The Agricultural Products Processing Promotion Center, Shijiazhuang 050011, China
  • Received:2020-05-21 Published:2020-12-28

摘要: 为了加快辣椒抗疫病新品种选育进程,选育抗疫病品种是防止或减轻辣椒疫病危害的有效途径。随着分子生物学的不断发展,辣椒分子标记辅助育种成为选育抗病品种快速有效的方法。利用分子标记技术对40份辣椒材料进行了抗疫病基因的分子标记筛选,同时对已报道的6个抗疫病分子标记进行鉴定。鉴定结果表明:3个STS抗疫病分子标记Phy-1Phy-4Phy-6扩增出特异性目标条带,共鉴定出18份抗疫病材料和2份感病材料。其中,引物Phy-1筛选出抗病材料14份,Phy-4筛选出抗病材料7份,同时含有2个抗病基因的有3份,Phy-6筛选出感病材料2份。利用分子标记筛选抗病资源,可有效提高辣椒抗疫病分子育种的进程,为加快辣椒抗疫病新品种选育及产业发展奠定了基础。

关键词: 辣椒, 疫病, 分子标记, 筛选, 检测

Abstract: In order to speed up the selection and breeding new varieties of resistance to Phytophthora blight peppers,breeding resistant varieties is an effective way to prevent or reduce the damage of pepper disease.With the development of molecular biology,molecular marker-assisted breeding of pepper has become a fast and effective method for breeding resistant varieties.The genes of resistance to Phytophthora bligh twere identified in 40 pepper materials by molecular marker technology,and 6 molecular markers of resistance to Phytophthora blight were screened at the same time.The identification results showed that three STS molecular markers Phy-1,Phy-4 and Phy-6 had target strips,and a total of 18 materials of resistance to Phytophthora blight and 2 susceptible materials were identified.Among them,14 disease-resistant materials were screened by Phy-1,7 disease-resistant materials were screened by Phy-4. 3 materials containing 2 disease-resistant genes,and 2 disease-susceptible materials was screened by Phy-6.Using molecular markers to screen disease-resistant resources can effectively improve the process of molecular breeding of disease-resistant in pepper and lay a foundation for accelerating the breeding of new disease-resistant varieties and industrial development of pepper.

Key words: Pepper, Phytophthora blight, Screening, Detection, Molecular marker

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引用本文

孟雅宁, 严立斌, 张红肖, 赵海涛, 范妍芹. 辣椒抗疫病基因分子标记的筛选及检测[J]. 华北农学报, 2020, 35(S1): 338-341. doi: 10.7668/hbnxb.20191405.

MENG Yaning, YAN Libin, ZHANG Hongxiao, ZHAO Haitao, FAN Yanqin. Screening and Detection of Resistance to Phytophthora Blight Gene in Pepper Using Molecular Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 338-341. doi: 10.7668/hbnxb.20191405.

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