基于玉米BC2F2群体的穗部性状QTL分析

doi:10.7668/hbnxb.2010.04.016

华北农学报 ›› 2010, Vol. 25 ›› Issue (4): 73-77. doi: 10.7668/hbnxb.2010.04.016

所属专题： 玉米；

基于玉米BC2F2群体的穗部性状QTL分析

胡利宗¹, 刘均革², 郭晋杰¹, 赵永锋¹, 祝丽英¹, 宋占权³, 陈景堂¹

1. 河北农业大学, 河北保定 071001;
2. 天津静海良种场, 天津 301600;
3. 保定学院, 河北保定 071051

收稿日期:2010-07-02 出版日期:2010-08-28
通讯作者: 陈景堂(1967- )，男，河北平泉人，在读博士，教授，主要从事玉米遗传育种研究。
作者简介:胡利宗(1979- ),男,河南洛阳人,硕士,主要从事玉米遗传育种研究.
基金资助:
河北省"十一五"科技支撑计划(20061148001);河北省自然科学基金项目资助(C2006000441);河北省教育厅自然科学研究项目(Z2008115)

QTL Analysis of Ear Traits Based on BC2F2 Population in Maize(Zea may L.)

HU Li-zong¹, LIU Jun-ge², GUO Jin-jie¹, ZHAO Yong-feng¹, ZHU Li-ying¹, SONG Zhan-quan³, CHEN Jing-tang¹

1. College of Agronomy, Hebei Agricultural University, Baoding 071001, China;
2. Jinghai Seed Base, Tianjin 301600, China;
3. Baoding College, Baoding 071051, China

Received:2010-07-02 Published:2010-08-28

摘要/Abstract

摘要： 以农系110为受体、农系31为供体材料,采用回交法构建了样本容量为9株的BC2F2回交群体,选取126个均匀分布在10条染色体上的多态性SSR标记进行6个穗部性状QTL分析.结果表明,受体农系110的穗长、穗粗、穗行数、行粒数、百粒重和穗粒重6个穗部性状表型值分别增加了9.4%,9.6%,4.1%,2.7%,0.3%和4.4%;构建一张含126个SSR标记的玉米分子标记连锁遗传图谱,总长度为2 317.4 cM,平均区间长18.4 cM;采用复合区间作图法,定位了19个与玉米穗部性状有关的QTL,其中穗长QTL 4个,穗粗QTL 2个,行粒数QTL 1个,穗行数QTL 3个,百粒重QTL 4个,穗粒重QTL 个.

关键词: 玉米, 数量性状位点, 穗部性状, 作图群体

Abstract: The mapping population derived from a cross of Nongxi 110 as receptor and Nongxi 531 as donor，including 95 BC₂F₂ individuals，was developed by back cross breeding program in maize (Zea may L.) . The 126 polymorphic SSR primer pairs between parents，uniformly covering the whole genome，were used for genotyping of 95 BC₂F₂ individuals. The correlation and OTL analysis for ear traits were performed in this mapping population. The correlative result indicated that there is a significant positive correlation between ear length，ear row number of grain，row kernel number and ear weight，a significant positive correlation between ear length and row kernel number，but a significant negative correlation between ear length and ear diameter. According to CIM mapping method，the total 19 QTL for 6 ear traits were identified and located on in the chromosomes which included 4 QTL for ear length，2 QTL for ear diameter，1 QTL for row kernel number，3 QTL for ear row number，4 QTL for hundred kernel weight，5 QTL for ear kernel weight.

Key words: Maize, Quantitative trait loci (QTL), Ear trait, Mapping population

中图分类号:

S13.03

胡利宗, 刘均革, 郭晋杰, 赵永锋, 祝丽英, 宋占权, 陈景堂. 基于玉米BC2F2群体的穗部性状QTL分析[J]. 华北农学报, 2010, 25(4): 73-77. doi: 10.7668/hbnxb.2010.04.016.

HU Li-zong, LIU Jun-ge, GUO Jin-jie, ZHAO Yong-feng, ZHU Li-ying, SONG Zhan-quan, CHEN Jing-tang. QTL Analysis of Ear Traits Based on BC2F2 Population in Maize(Zea may L.)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 73-77. doi: 10.7668/hbnxb.2010.04.016.

http://www.hbnxb.net/CN/Y2010/V25/I4/73

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