摘要： 为了研究二系杂交小麦HMW-GS类型与蛋白质含量、沉淀值、面团形成时间和衰弱角斜率等品质性状的关系,采用SDS-PAGE方法分析了杂交种的高分子量麦谷蛋白亚基(HMW-GS)的组成。结果表明,在试验材料中普遍存在有劣质亚基N和2+12,它们的频率分别是60.4%,83.0%。在HMW-GS与F1籽粒品质性状关系的研究中发现,各位点对蛋白质含量的贡献率是Glu-B1> Glu-A1> Glu-D1 对沉淀值贡献率是Glu-B1> Glu-D1> Glu-A1 对形成时间和衰落角斜率的贡献率是Glu-D1> Glu-B1> Glu-A1。总体来说,位点按对品质的贡献率大小依次为Glu-B1>Glu-D1> Glu-A1。就单个亚基而言Glu-D1位点,杂合2+12/5+10>纯合2+12 Glu-A1位点蛋白质含量、SDS沉淀值和形成时间均为N/N> N/1 在Glu-B1位点,蛋白质含量是7+8/17+18=7+9/17+18=7+8/7+9=7+8/7+8> 7+9/7+9 沉淀值的大小依次为7+8/17+18≥7+9/17+18=7+8/7+9=7+8/7+8≥7+9/7+9 形成时间是7+8/17+18> 7+9/17+18=7+8/7+9=7+8/7+8=7+9/7+9 衰弱角斜率大小是7+9/17+18≥7+8/17+18=7+9/7+9≥7+8/7+9=7+8/7+8。在不同亚基组合中,具有N/N,7+8/7+8,2+12/5+10亚基的品质最好。杂合位点5+10亚基对2+12亚基的品质具有补偿效应。
关键词:
二系杂交小麦,
品质,
十二烷基硫酸钠-聚丙烯胺凝胶电泳,
高分子量麦谷蛋白亚基
Abstract: In order to establish the relationships between high molecular weight glutenin subunits(FIMW-GS) typeand the flour quality parameters of protein content,sedimentation plume,cbugh development time and feeble angle slopein two-line system hybrid wheat,the SDS PAGE was used to analyze the compositions of FIMW CAS of hybrid wheat seeds.The result indicated that the inferior subunits N and 2+12 existed universally in the experimental materials. Their fre-quency were 60.4%and 83. 0%,respectively. The relationships between FIMW CAS type and the flour quality charactersof F1 were ranked as follows：in protein content Clu-BI>Clu-AI>Clu-DI;in sedimentation plume Clu-BI>Clu-DI>Glu-AI;in cbugh development time andfeeble angle slope Glu-DI>Cl u-B 1>Clu-AI,respectively. In all,the orderof contributions to the flour quality character was Glu-BI>Clu-DI>Clu-A 1.As far as individually subunit was con-cerned,at Clu-DI,the heterogeneous 2+12/ 5+10 subunits was higher than homogeneous 2+12 subunits;at Clu-AI,the order in sedimentatiomolume,protein content and cbugh development time was N/ N>N/ 1;at Clu-BI,in proteincontent,it was ranked as 7+8/ 17+18=7+9/ 17+18=7+8/ 7+9=7+8/ 7+8>7+9/ 7+9;in sedimentatiomol-ume,7+8/ 17+18 /7+9/ 1>7+9/ 17+18=7+8/ 7+9=7+8/ 7+8=7+9/ 7+9;in feeble angle slope was 7+9/ 17+18≧7+8/ 17+18=7+9/ 7+9≧+8/ 7+9=7+8/ 7+8. As a whole,HMW GS combination N/ N,7+8/ 7+8,2+12/ 5+10 was the bestquality in all of the brid wheat.This can be ascribed as the compensate effects of the good quality HMW-GS5+10 onthe worse ones 2+12 in heterozygous condition.
Key words:
Two-lines system hybrid wheat,
Quality,
SDS PAGE,
HMW CAS
中图分类号:
张之为, 张立平, 赵昌平, 樊明寿, 单福华, 苏青, 刘建平, 张风廷, 叶志杰. 二系杂交小麦HMW-GS组成与品质关系的初步研究[J]. 华北农学报, 2009, 24(1): 149-153. doi: 10.7668/hbnxb.2009.01.033.
ZHANG Zhi-wei, ZHANG Li-ping, ZHAO Chang-ping, Ming-shou, SHAN Fu-hua, SU Qing, LIU Jian-ping, ZHANG Feng-ting, YE Zhi-jie. The Preliminary Studies on Compositions of High Molecular Weight Glutenin Subunits andbTheir Effects on Quality in Hybrid Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(1): 149-153. doi: 10.7668/hbnxb.2009.01.033.