摘要： 应用RAPD技术,对我国不同产地的锦鸡儿属植物30份材料进行遗传多样性分析,从40个随机引物中筛选出15个有效引物,共扩增出87条DNA片段,其中74条为多态性条带,约占总数的84.1%。30份材料聚类结果可将供试材料分为4个类群:Ⅰ.中间锦鸡儿材料区,Ⅱ.小叶锦鸡儿材料区,Ⅲ.柠条锦鸡儿材料区和Ⅳ.秦晋锦鸡儿材料区,中间锦鸡儿、小叶锦鸡儿和柠条锦鸡儿种间差异不大,有较近的亲缘关系,它们与秦晋锦鸡儿的亲缘关系较远,同时反映出品种间关系与地理起源有一定的相关性。
关键词:
锦鸡儿属,
RAPD,
遗传多样性,
聚类分析
Abstract: Peashrub,in common name of Caragana Fabr,there is about 66 species of peashrub were recorded inChina,which are mainly distributed in arid and semi-arid areas of North China. All species of peashrub developed typicalbiological features,which are highly drought-tolerant and good shrubs for dun}fixation,animal feeding,and biological re-sources for fuel energy and fiber material.They are valuable plants for the ecolocal restoration of North China. It is veryimportant to analyze the genetic diversity of peashrub for protecting and utilizing them. In this paper,the genetic diversi-ties of 30 peashrubs form different places in North China were analyzed by RAPD markers. Among 87 RAPD bands ob-twined form 15 primes,74 bands were polymoiphic which occupied 84. 1%of the total. Cluster analysis showed that thetested materials of 30 peashrubs could be divided into 4 groups：population of C. intermedia;population of C. microphyl-la; population of C. korhinskii I am and population of C. purdomi,the relationships among population of C. intermedi-a,population of C. mdcroplla and population of C. korshdnskdd Item are nearer than population of C. purdomd,it alsoshowed that their relationships are ralated with the geographin
Key words:
Caragana Fabr,
RAPD,
Genetic diversity,
Cluster analysis
中图分类号:
段永红, 牛西午, 李素清, 孙毅. 锦鸡儿属植物遗传多样性的RAPD分析[J]. 华北农学报, 2009, 24(1): 143-148. doi: 10.7668/hbnxb.2009.01.032.
DUAN Yong-hong, NIU Xi-wu, LI Su-qing, SUN Yi. Genetic Diversity Analysis of Peashrub in China by RAPD[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(1): 143-148. doi: 10.7668/hbnxb.2009.01.032.