摘要： 为了解西瓜强雌性性状的遗传规律,以强雌性系BG1为母本,分别与不同的西瓜品系杂交、自交及回交,共获得5个F₁群体、1个F₂群体、1个回交群体BC₁R34,以及1个回交转育品系(BC4S3)。5个F₁1群体植株全部表现为正常花性型 经χ²测验F₂分离群体中强雌性型与普通花性型的分离符合1∶3的分离比率 与普通花性型回交后代的BC₁R34表现为普通花性型 回交转育得到的BC₄S₃品系表现为强雌性型,雌花率高达77.9%。试验结果表明,该强雌性状主要受单隐性核基因控制,但根据F₂单株雌花率的分布情况,认为还可能存在微效基因的作用。对两对近等基因系BG1和BG2及BC₄S₃和R34进行RAPD分析,共使用333条随机引物,经过进一步F₂验证在强雌性系中获得一条稳定扩增的片段s1454-1100。

关键词: 西瓜, 强雌性系, 遗传分析, RAPD

Abstract: The objective of this Experiment was to investigate the inheritance of subgynoecious trait in watermekn.Five F₁ combinations, one F₂ segregation population, one backcross population(BC₁R34)and one transferred subgyrxeciousline( BC₄S₃) were developed by crossing, selfing and backcross betwe subgynoecious line( BG1) and other watermelon inbred lines,respectively. All the plants of F₁populations expressed monoecious, similar to their male parents; F₂ populationsegregated into two phenotypes normal monoecious type and subgynoecious type in ratio of 1: 3 throughx²test;as wellthe plants of backcross population BC₁R34 complet dyexpressed morx>ecious. In addition, trarrferxed sudgynoecious lineBC₄S₃was suhgynoecimr type that proprced high female flower,proportion to 77.9%.These results irxlicated that sugnoecious in watermelon was controlled by a single recessive gene. According to the frequen listridution of female,proportion in F₂, it was considered that there might be other genes modifief this subgyrxecious trait. Two pairs ofNILs, BG1 versus BG2, and BC₄S₃ versus R34 was studied lry the RAPD technique out of 333 random primers. A specificamplified fragment s1454_ 1100 in subgynoecious line was found out through verifing the F₂individuals.

Key words: Watermelon, Subgynoecious line, Inheritance analysis, RAPD

中图分类号: 

• S651