玉米矮秆突变体K125d的遗传鉴定

doi:10.7668/hbnxb.20190244

摘要/Abstract

摘要： 积极发掘新的玉米矮秆资源并进行研究和利用，有利于玉米矮化育种。对比研究了矮秆突变体K125d和同源自交系K211之间的形态差异，通过与7个不同株高自交系配制正反交F₁，回交B₁、B₂和自交F₂群体，分析突变体K125d矮秆性状的遗传模式，其矮秆基因定位用BSA-SSR标记法。结果表明，与K211相比，K125d的生育期极显著增长，株高极显著降低；叶片重叠密集，叶数和叶宽极显著增加，叶片夹角极显著降低。所有群体在2个不同生态点试验结果一致，正反交F₁均为高秆；7个B₁群体和F₂群体株高分离比例分别符合1 ∶ 1和3 ∶ 1；除K123d外，6个B₂群体均为高秆，表明突变体K125d的株高由1对隐性细胞核基因控制，暂命名为d125。以（K125d×K236） F₂为定位群体，将基因d125定位于1号染色体SSR标记umc2569和umc1278之间，其距离为6.6，5.1 cM。以br2基因模型GRMZM2G315375克隆d125发现， d125在模型第1 651个碱基处有一个9 bp片段插入，在第6 438碱基处有一个232 bp片段缺失，缺失导致移码突变，造成功能位点缺失可能是导致转运功能丧失的主要原因。

关键词: 玉米, 矮秆突变体, K125d, 遗传分析, 基因定位, 基因克隆

Abstract: It is of great significance to explore, study and utilize the new dwarf maize resources in maize dwarf breeding. The study compared and analyzed the morphological differences between dwarf mutant K125d and homologous K211 inbred lines. The reciprocal F₁, B₁, B₂ and F₂ populations between K125d and seven inbred lines with different heights were used to analyze the genetic model of dwarf characters in mutant K125d and to locate the dwarf gene by BSA-SSR labeling method. Compared with K211, K125d displayed significantly longer growth period, shorter plant height, dense leaf overlapping, increased leaf number and leaf breadth, and decreased leaf angle. The experiment results were consistent in all populations from two different ecological sites. The height of reciprocal F₁ was taller. The separation proportions of B₁ and F₂ populations were 1:1 and 3:1, respectively. The heights of B₂ populations were tall in six populations except K123d. The plant height of dwarf mutant K125d was controlled by a recessive nuclear gene, which was tentatively named d125. The F₂ population from k125d×K236 was used as the locating population. The dwarf gene d125 was initially located on the long arm of chromosome 1, between SSR markers umc2569 and umc1278 with the genetic distances of 6.6,5.1 cM, respectively. It was concluded that d125 was cloned from gene ID GRMZM2G315375, which had an insertion of 9 bp fragment at +1 651 locus and a deletion of 232 bp fragment at +6 438 locus compared to K211. The deletion led to frameshift mutation, and the loss of functional sites might be the main reason for the loss of transport function.

Key words: Maize, Dwarf mutant, K125d, Genetic analysis, Gene location, Gene cloning

中图分类号:

S513.03

石海春, 陈立, 李开兵, 余学杰, 袁继超, 曲比伍合, 柯永培. 玉米矮秆突变体K125d的遗传鉴定[J]. 华北农学报, 2020, 35(1): 65-72. doi: 10.7668/hbnxb.20190244.

SHI Haichun, CHEN Li, LI Kaibing, YU Xuejie, YUAN Jichao, QUBI Wuhe, KE Yongpei. Genetic Analysis of Maize Dwarf Mutant K125d[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(1): 65-72. doi: 10.7668/hbnxb.20190244.

http://www.hbnxb.net/CN/Y2020/V35/I1/65

参考文献

[1] 曹庆军, 杨粉团, 姜晓莉, 陈莫军, 李贺, 于洪浩, 鲁建华, 张兆琴, 薄晓杰, 李刚. 玉米抗茎倒能力评价及理想株型[J]. 东北农业科学, 2017, 42(2):17-21.doi:10.16423/j.cnki.1003-8701.2017.02.005.
Cao Q J, Yang F T, Jiang X L, Chen M J, Li H, Yu H H, Lu J H, Zhang Y Q, Bo X J, Li G. Evaluation of stem lodging resistance and ideal plant type of lodging resistant maize varieties[J]. Journal of Northeast Agricultural Sciences, 2017, 42(2):17-21.
[2] 何川. 抗倒伏玉米矮生系的选育与利用[J]. 杂粮作物, 2010, 30(6):387-388.doi:10.3969/j.issn.2095-0896.2010.06.004.
He C. Breeding and utilizing of dwarf maize lodging[J]. Rain Fed Crops, 2010, 30(6):387-388.
[3] 周颖, 顾万荣, 赵猛, 佟桐, 刘笑鸣, 李彩凤, 李晶, 魏湜. 黑龙江省不同熟期春玉米品种茎秆特性及机收指标差异[J]. 华北农学报, 2017, 32(S1):140-146.doi:10.7668/hbnxb.2017.
S1.025.
Zhou Y, Gu W R, Zhao M, Tong T, Liu X M, Li C F, Li J, Wei T. Differences of stalk characteristics and grain mechanically harvesting qualities of different maturing-type spring maize in Heilongjiang Province[J]. Acta Agriculturae Boreali-Sinica, 2017, 32(S1):140-146.
[4] 彭长俊, 崔士友. 玉米育种技术体系的构建及有关问题的讨论[J]. 农学学报, 2018, 8(4):1-7.
Peng C J, Cui S Y. The construction of technology system of maize breeding and the discussion on relevant problems[J]. Journal of Agriculture, 2018, 8(4):1-7.
[5] 崔绍平, 孙世珍, 徐有, 郑积德, 李洪杰. 玉米br-2矮生基因利用的研究[J]. 华北农学报, 1990, 5(2):7-12.doi:10.3321/j.issn:1000-7091.1990.02.002.
Cui S P, Sun S Z, Xu Y, Zheng J D, Li H J. A study on util ization of a dwarf gene br-2 in maize breeding[J]. Acta Agriculturae Boreali-Sinica, 1990, 5(2):7-12.
[6] 阎淑琴. 矮生玉米的遗传与育种[J]. 玉米科学, 2000, 8(2):36-37, 45.doi:10.3969/j.issn.1005-0906.2000.02.009.
Yan S Q. Inheritance and breeding of dwarf type maize[J]. Journal of Maize Sciences, 2000, 8(2):36-37, 45.
[7] 何川, 郑祖平, 谢树果, 李钟, 刘代惠. 隐性单基因br-2玉米矮生系的选育[J]. 中国农业科学, 2009, 42(8):2978-2981.doi:10.3864/j.issn.0578-1752.2009.08.042.
He C, Zheng Z P, Xie S G, Li Z, Liu D H. Breeding of the maize monogenic br-2 dwarf lines[J]. Scientia Agricultura Sinica, 2009, 42(8):2978-2981.
[8] 樊景胜. 玉米矮生基因遗传及其利用[J]. 黑龙江农业科学, 1999(1):29-30.doi:10.11942/j.issn1002-2767.1999.01.0029.
Fan J S. Inheritance and utilization of dwarf gene in maiz[J].Heilongjiang Agricultural Sciences, 1999(1):29-30.
[9] 田齐建, 乔治军, 董存吉, 穆志新. 玉米矮化育种研究进展及发展前景[J]. 山西农业科学, 2003, 31(2):23-26.doi:10.3969/j.issn.1002-2481.2003.02.006.
Tian Q J, Qiao Z J, Dong C J, Mu Z X. Review on research of maize breeding for dwarfness and its development prospect[J]. Journal of Shanxi Agricultural Sciences, 2003, 31(2):23-26.
[10] 邱正高, 杨华, 袁亮, 张亚勤, 张采波, 汤玲, 荣廷昭, 曹墨菊. 一份新选玉米矮秆突变体的鉴定与遗传分析[J]. 华北农学报, 2015, 30(6):112-118.doi:10.7668/hbnxb.2015.06.017.
Qiu Z G, Yang H, Yuan L, Zhang Y Q, Zhang C B, Tang L, Rong T Z, Cao M J. Identification and genetic analysis of a new dwarf mutant in maize[J]. Acta Agriculturae Boreali-Sinica, 2015, 30(6):112-118.
[11] 徐敏, 石海春, 余学杰, 谭义川, 柯永川, 赵长云, 柯永培. 一个玉米矮秆突变体K123d的遗传鉴定[J]. 植物遗传资源学报, 2017, 18(1):155-163.doi:10.13430/j.cnki.jpgr.2017.01.020.
Xu M, Shi H C, Yu X J, Tan Y C, Ke Y C, Zhao C Y, Ke Y P. Genetic identification of a dwarf mutant K123d in maize(Zea mays L.)[J]. Journal of Plant Genetic Resources, 2017, 18(1):155-163.
[12] 牛群凯, 杨聪, 时子文, 曹墨菊. 玉米太空诱变核不育突变体矮化性状的QTL定位及分析[J]. 四川农业大学学报, 2018, 36(4):429-435, 480.doi:10.16036/j.issn.1000-2650.2018.04.002.
Niu Q K, Yang C, Shi Z W, Cao M J. QTL mapping of dwarf-associated traits in the maize male sterile mutant obtained by space flight[J]. Journal of Sichuan Agricultural University, 2018, 36(4):429-435, 480.
[13] 刘忠祥, 杨梅, 殷鹏程, 周玉乾, 何海军, 邱法展. 玉米株高主效QTL qPH3.2 精细定位及遗传效应分析[J]. 作物学报, 2018, 44(9):1357-1366.doi:10.3724/SP.J.1006.2018.01357.
Liu Z X, Yang M, Yin P C, Zhou Y Q, He H J, Qiu F Z. Fine mapping and genetic effect analysis of a major QTL qPH3.2 associated with plant height in maize(Zea mays L.)[J]. Acta Agronomica Sinica, 2018, 44(9):1357-1366.
[14] 张素梅, 刘凤军, 刘保申, 王立静, 董树亭. 新的玉米显性矮秆基因的发现及初步分析[J]. 玉米科学, 2007, 15(3):15-18.doi:10.3969/j.issn.1005-0906.2007.03.004.
Zhang S M, Liu F J, Liu B S, Wang L J, Dong S T. Discovery of a new dominant dwarf gene in maize and its preliminary study[J]. Journal of Maize Sciences, 2007, 15(3):15-18.
[15] 王益军, 苗楠, 施亚婷, 邓德祥, 卞云龙. 一份玉米显性矮秆突变体的遗传分析[J]. 华北农学报, 2010, 25(5):90-93.doi:10.7668/hbnxb.2010.05.019.
Wang Y J, Miao N, Shi Y T, Deng D X, Bian Y L. Genetic analysis of a dominant dwarf mutant in maize[J]. Acta Agriculturae Boreali-Sinica, 2010, 25(5):90-93.
[16] Wang Y J, Deng D X, Ding H D, Xu X M, Zhang R, Wang S X, Bian Y L, Yin Z T, Chen Y. Gibberellin biosynthetic deficiency is responsible for maize dominant dwarf11(D11) mutant phenotype:physiological and transcriptomic evidence[J]. PLoS One, 2013, 8(6):e66466.doi:10.1371/journal.pone.0066466.
[17] Multani D S, Briggs S P, Chamberlin M A, Blakeslee J J, Murphy A S, Johal G S. Loss of an MDR transporter in compact stalks of maize br2 and sorghum dw3 mutants[J]. Science, 2003, 302(5642):81-84.doi:10.1126/science.1086072.
[18] Bensen R J, Johal G S, Grane V C, Tossberg J T, Schnable P S, Meeley R B, Briggs S P. Cloning and characterization of the maize An1 gene[J]. The Plant Cell,1995, 7(1):75-84.doi:10.1105/tpc.7.1.75.
[19] Peng J R, Rivhards D E, Hartley N M, Murphy G P, Devos K M, Flintham J E, Beales J, Fish L J, Worland A J, Pelica F, Sudhakar D, Christou P, Snape J W, Gale M D, Harberd N P. Green revolution genes encode mutant gibberellin response modulators[J]. Nature, 1999, 400(6741):256-261.doi:10.1038/22307.
[20] Lawit S J, Wych H M, Xu D P, Kundu S, Tomes D T. Maize DELLA proteins dwarf plant8 and dwarf plant9 as modulators of plant development[J]. Plant & Cell Physiology, 2010, 51(11):1854-1868.doi:10.1093/pcp/pcq153.
[21] Winkler R G, Helentjaris T. The maize Dwarf3 gene encodes a cytochrome P450-mediated early step in gibberellin biosynthesis[J]. The Plant Cell, 1995, 7(8):1307-1317.doi:10.1105/tpc.7.8.1307.
[22] 王立静. 玉米矮秆基因 Dt 和坏死基因 net-t 的图位克隆与功能分析[D]. 泰安:山东农业大学, 2012.doi:10.7666/d.d224451.
Wang L J. Map-based cloning and functional analysis of dwarf gene Dt and necrotic gene net-t in maize[D].Taian:Shandong Agricultural University, 2012.
[23] Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis:a rapid method to detect markers in specific genomic regions by using segregating populations[J]. Proceedings of the National Academy of Sciences, 1991, 88(21):9828-9832.doi:10.2307/2357899.
[24] 刘仁虎, 孟金陵. MapDraw, 在Excel中绘制遗传连锁图的宏[J]. 遗传, 2003, 25(3):317-321.doi:10.3321/j.issn:0253-9772.2003.03.019.
Liu R H, Meng J L. MapDraw:a microsoft excel macro for drawing genetic linkage maps based on given genetic linkage data[J]. Hereditas, 2003, 25(3):317-321.
[25] 李钟, 郑祖平, 张国清, 何川. 矮生玉米自交系的选育和利用[J]. 玉米科学, 2006, 14(1):76-78.doi:10.3969/j.issn.1005-0906.2006.01.023.
Li Z, Zheng Z P, Zhang G Q, He C. Breeding and application on the inbred line of dwarf maize[J]. Journal of Maize Sciences, 2006, 14(1):76-78.
[26] 李忠南, 王克伟, 王越人, 邬生辉, 李光发. 玉米品种先玉335的血缘系谱及主要农艺性状遗传分析[J]. 玉米科学, 2018, 26(3):32-39.doi:10.13597/j.cnki.maize.science.20180307.
Li Z N, Wang K W, Wang Y R, Wu S H, Li G F. Genetic analysis on pedigree and agronomic characters of maize variety Xianyu 335[J]. Journal of Maize Sciences, 2018, 26(3):32-39.
[27] Sánchez-fernández R, Davies T G E, Coleman J O D, Rea P A. The Arabidopsis thaliana ABC protein superfamily, a complete inventory[J]. Journal of Biological Chemistry, 2001, 276(32):30231-30244.doi:10.1074/jbc.M103104200.
[28] Pilu R, Cassani E, Villa D, Curiale S, Panzeri D, Badone F C, Landoni M. Isolation and characterization of a new mutant allele of brachytic 2 maize gene[J]. Molecular Breeding, 2007, 20(2):83-91.doi:10.1007/s11032-006-9073-7.
[29] Xing A Q, Gao Y F, Ye L F, Zhang W P, Cai L C, Ade C, Liaca V, Johnson B, Liu L, Yang X H, Kang D M, Yan J B, Li J S. A rare SNP mutation in Brachytic2 moderately reduces plant height and increases yield potential in maize[J]. J Exp Bot, 2015, 66(13):3791-3802.doi:10.1093/jxb/erv182.
[30] Jamie S,Guillaume T, Houry W A. The AAA+ superfamily of functionally diverse proteins[J]. Genome Biology, 2008, 9(4):1-8.doi:10.1186/gb-2008-9-4-216.
[31] Azzaria M, Schurr E, Gros P. Discrete mutations introduced in the predicted nucleotide-binding sites of the mdr1 gene abolish its ability to confer multidrug resistance[J]. Molecular & Cellular Biology, 1989, 9(12):5289-5297.doi:10.1128/MCB.9.12.5289.
[32] Beaudet L, Gros P. Functional dissection of P-glycoprotein nucleotide-binding domains in chimeric and mutant proteins. Modulation of drug resistance profiles[J]. Journal of Biological Chemistry, 1995, 270(29):17159-17170.doi:10.1074/jbc.270.29.17159.

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