华北农学报 ›› 2007, Vol. 22 ›› Issue (2): 29-33. doi: 10.3321/j.issn:1000-7091.2007.02.008

所属专题: 玉米 生物技术

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玉米抽穗期亲本及其杂交种间基因差异表达与杂种优势的关系

雷海英1, 孙毅1,2, 阎敏1, 段永红1   

  1. 1. 山西大学生物工程学院, 山西 太原 030006;
    2. 山西省农业生物技术研究中心, 山西 太原 030031
  • 收稿日期:2006-11-05 出版日期:2007-04-28
  • 通讯作者: 孙毅(1953-),男,黑龙江哈尔滨人,研究员,主要从事植物基因工程研究工作。
  • 作者简介:雷海英(1978-),女,山西平遥人,在读硕士,主要从事玉米分子标记育种研究工作。
  • 基金资助:
    山西省留学回国人员资助项目(晋出留办学(97)1号)

Differential Gene Expression Between Hybrids and Their Parental Inbreds are Correlated with Heterosis in Heading Stage Functional Leaves of Maize

LEI Hai-ying1, SUN Yi1,2, YAN Min1, DUAN Yong-hong1   

  1. 1. School of Biology Engineering, Shanxi University, Taiyuan 030006, China;
    2. Agricultural Biotechnology Research Center of Shanxi Province, Taiyuan 030031, China
  • Received:2006-11-05 Published:2007-04-28

摘要: 为探讨玉米杂种优势形成的分子机理,选用了8个玉米自交系和它们的5个杂交种,采用mRNA差异显示技术分析了杂种和亲本之间抽穗期叶片的基因表达差异模式。结果表明:所选用的杂交种与自交系之间的基因表达模式可以分为5种类型:杂种特异表达型(W1)、单亲显性表达型(W2)、双亲表达沉默型(W3)、单亲沉默表达型(W4)、亲本和杂交种表达一致型(W5),所占比例分别为2.81%,16.92%,11.79%,4.25%和64.23%。还对每种差异表达模式与12个杂种性状表现和中亲优势进行了相关分析。

关键词: 玉米, 杂种优势, mRNA差异显示

Abstract: To explore the genetic mechanism of heterosis, 8 maize inbreds line and their 5 hybrids were employed and analyzed the patterns of differential gene expression in functional leaves at heading stage. The results revealed that the differential expression patterns between inbreds and their hybrids are as following: bands observed only in hybrids (W1), bands only present in one parent (W2), bands present in one parent and F1 but absent in the other parent (W3), bands present in both parents but absent in F1 (W4), bands present in both parents and F1 (W5), which accounted for 2.81 %, 16.92 %, 11.79 %, 4.25 % and 64.23 %, respectively. The correlations between differential gene expression patterns and the mid-parent heterosis of twelve agronomic traits were estimated.

Key words: Maize, Heterosis, mRNA differential display

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引用本文

雷海英, 孙毅, 阎敏, 段永红. 玉米抽穗期亲本及其杂交种间基因差异表达与杂种优势的关系[J]. 华北农学报, 2007, 22(2): 29-33. doi: 10.3321/j.issn:1000-7091.2007.02.008.

LEI Hai-ying, SUN Yi, YAN Min, DUAN Yong-hong. Differential Gene Expression Between Hybrids and Their Parental Inbreds are Correlated with Heterosis in Heading Stage Functional Leaves of Maize[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(2): 29-33. doi: 10.3321/j.issn:1000-7091.2007.02.008.

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