华北农学报 ›› 2004, Vol. 19 ›› Issue (3): 38-42. doi: 10.3321/j.issn:1000-7091.2004.03.010

所属专题: 水稻 盐碱胁迫 生物技术

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盐胁迫下转甜菜碱醛脱氢酶基因水稻幼苗耐盐生理的研究

孙耀中1, 东方阳1, 陈受宜2, 杨晓玲1, 刘永军1, 郭学民1   

  1. 1. 河北科技师范学院, 生命科学系, 河北昌黎 066600;
    2. 中国科学院遗传研究所, 北京 100000
  • 收稿日期:2003-08-25 出版日期:2004-09-28
  • 作者简介:孙耀中(1958-),男,河北乐亭人,教授,主要从事生物统计学教学与作物遗传育种研究.
  • 基金资助:
    河北省秦皇岛市科学技术局重点资助课题(2002-04)

Study on the Physiology of Transgenic Rice Seedlings with Gene of Betaine Aldehyde DehydrogeNa+se Gene under Salt Stress

SUN Yao-zhong1, DONG Fang-yang1, CHEN Shou-yi2, YANG Xiao-ling1, LIU Yong-jun1, GUO Xue-min1   

  1. 1. Department of Life Science, Hebei Teacher’s College of Science and Technology, Changli 066600, China;

    2. Institute of Genetice, China Academy of Science, Beijing 100000, China
  • Received:2003-08-25 Published:2004-09-28

摘要: 在Na+Cl浓度0,3.0,.0,7.0 g/L下,对转甜菜碱醛脱氢酶(BADH)基因水稻的两个品系1-22,2-7及其受体亲本中花8号进行幼苗耐盐性试验.结果表明:转BADH基因水稻比其受体亲本耐盐性强,在高Na+Cl浓度(.0,7.0 g/L)胁迫下,甜菜碱醛脱氢酶基因可以提高盐胁迫下水稻幼苗过氧化氢酶(CAT)活性,提高根系活力和叶绿素含量,稳定细胞膜的透性,降低植株体内Na++的积累,从而减轻幼苗受盐害的程度.盐胁迫下水稻幼苗的CAT活性、SNa++/K+、叶Na++/K+、叶绿素含量是影响幼苗生长的主要生理指标.

关键词: 水稻, 甜菜碱醛脱氢酶基因, 盐胁迫, 耐盐生理

Abstract: Salt stress experiments of rice seedlings of two lines with a gene encoding for BADH,51一22,52 一7 and the recipient Zhonghua No. 8,were conducted under the Na+CI concentration of 0,3. 0,5. 0,7. 0 g/L. The results showed that the rice cultivars with a gene encoding for BADH demonstrated higher salt tolerance than its recipient;The gene encoding for BADH could reduce the salt injury by increasing CAT activity,root ac-tivity,chlorophyll content and cell membrane osmosis,and by decreasing the Na+十concentration in the seedlings under high Na+CI stress (Na+CI concentration:5.0, 7. 0 g/L) ; The CAT activity, SN。十/K* and chlorophyll content are the main physiological indexes affecting the seedling growth.

Key words: Rice, Betaine aldehyde dehydrogenase, Salt stress, Salt tolerance physiology

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引用本文

孙耀中, 东方阳, 陈受宜, 杨晓玲, 刘永军, 郭学民. 盐胁迫下转甜菜碱醛脱氢酶基因水稻幼苗耐盐生理的研究[J]. 华北农学报, 2004, 19(3): 38-42. doi: 10.3321/j.issn:1000-7091.2004.03.010.

SUN Yao-zhong, DONG Fang-yang, CHEN Shou-yi, YANG Xiao-ling, LIU Yong-jun, GUO Xue-min. Study on the Physiology of Transgenic Rice Seedlings with Gene of Betaine Aldehyde DehydrogeNa+se Gene under Salt Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2004, 19(3): 38-42. doi: 10.3321/j.issn:1000-7091.2004.03.010.

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