华北农学报 ›› 2001, Vol. 16 ›› Issue (3): 12-16. doi: 10.3321/j.issn:1000-7091.2001.03.003

所属专题: 玉米

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影响玉米花药培养效率的因素研究

原玉香, 耿建峰, 张晓伟, 蒋武生, 韩永平, 高睦枪   

  1. 河南省农业科学院生物技术研究所, 河南郑州 450002
  • 收稿日期:2001-01-09 出版日期:2001-09-28
  • 作者简介:原玉香(1970- ),女,助理研究员,农学硕士,主要从事生物技术遗传育种研究工作。
  • 基金资助:
    国家自然科学基金资助项目(39700091)

A Study on Factors Affecting on Embryoid Induction Efficiency in Maize(Zea mays L.)Anther Culture

YUAN Yuxiang, GENG Jianfeng, ZHANG Xiaowei, JIANG Wusheng, HAN Yongping, GAO Muqiang   

  1. Horticultural Institute, Henan Academy of Agricultural Sciences, Zhengzhou Henan 450002, China
  • Received:2001-01-09 Published:2001-09-28

摘要: 对影响玉米花粉胚诱导的因素进行了研究。结果表明:花药接种前对雄穗进行℃低温预处理15~21d效果较好;接种后高温(33℃)连续处理花药,胚出现早、质量好且效率高;活性炭(0.5g/L)加入培养基中能显著地提高胚诱导效率。此外,还对基因型间差异进行了比较分析。

关键词: 玉米, 花药培养, 温度处理, 胚诱导率

Abstract: Some factors affecting on efficiency of embryoid induction in maize anther culture were studied.The results indicated that a 15 to 21 day cold pretreatment at 5℃ of tassels prior to anther inoculation had a good effect on the embryoid induction;Treating anthers with continuous high temperature at 33℃ was proved to be earlier,better and more embryoids induced than others;Adding activated charcoal to medium resulted in significantly improved embryoid yield;Differences between genotypes were also analyzed.

Key words: Zea mays L., Anther culture, Temperature treatment, Efficiency of embryoid induction

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引用本文

原玉香, 耿建峰, 张晓伟, 蒋武生, 韩永平, 高睦枪. 影响玉米花药培养效率的因素研究[J]. 华北农学报, 2001, 16(3): 12-16. doi: 10.3321/j.issn:1000-7091.2001.03.003.

YUAN Yuxiang, GENG Jianfeng, ZHANG Xiaowei, JIANG Wusheng, HAN Yongping, GAO Muqiang. A Study on Factors Affecting on Embryoid Induction Efficiency in Maize(Zea mays L.)Anther Culture[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2001, 16(3): 12-16. doi: 10.3321/j.issn:1000-7091.2001.03.003.

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