华北农学报 ›› 2000, Vol. 15 ›› Issue (4): 14-18. doi: 10.3321/j.issn:1000-7091.2000.04.004

所属专题: 玉米

• 论文 • 上一篇    下一篇

玉米改良单交种的聚丙烯酰胺凝胶电泳分析

陈景堂1, 池书敏1, 马占元2, 祝丽英1, 刘志增1, 孟义江1, 宋占权3, 魏俊杰1   

  1. 1. 河北农业大学农学院, 河北保定 071001;
    2. 河北省农林科学院, 河北石家庄 050051;
    3. 保定师范专科学校, 河北保定 071051
  • 出版日期:2000-12-28
  • 作者简介:陈景堂(1967-),男,讲师,硕士,主要从事教学和玉米遗传育种学的研究工作。
  • 基金资助:
    河北省农业厅资助项目(冀农科9716)

Analysis of Modified Single-cross of Maize with PAGE

CHEN Jing tang1, CHI Shu min1, MA Zhan yuan2, ZHU Li ying1, LIU Zhi zeng1, MENG Yi jiang1, SONG Zhan quan3, WEI Junjie1   

  1. 1. Department of Agronomy, Agricultural University of Hebei, Baoding 071001, China;
    2. Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050051, China;
    3. Baoding Teachers College,Baoding 071051, China
  • Published:2000-12-28

摘要: 利用改进的聚丙烯酰胺凝胶电泳技术对改良掖单19(8001×52106)、原掖单19(478×52106)及亲本8001、478、52106种子清蛋白(Albumin)进行了研究。改良掖单19共分离出18条清蛋白谱带,除了7条是双亲都有的公共带外,其余11条是分别来自于双亲的互补带,其中4条来自8001,7条来自52106,没有新生带的产生,在A区和B区表现双亲共显性,在C区表现偏父类型;原掖单19共分离出16条清蛋白谱带,比改良掖单19少两条,在谱带分布上也存在明显差别。改良掖单19与原掖单19的清蛋白谱带彼此不能相互替代。

关键词: 玉米, 姊妹系, 姊妹交种, 改良单交种, 原单交种, 清蛋白, 改进的聚丙烯酰胺凝胶电泳

Abstract: The research has been made on seed's albumin of modified Yiedan 19(8001×52106), Yiedan19(478×52106)and their parents 8001, 478, 52106 by improved polyacrylamide gel electrophoresis(PAGE). The results were as follows: 18 pieces of bands were in both of its parents, the rest were complementary type of the different bands of parents no other new bands presented. The bands were codominacance of parents in A. B zones, but expressed patroclial form in C zone.16 pieces of bands were derieved from Yiedan 19. There were significant differences not only in the number of bands but also in band distribution. Therefore, the bands of modified Yiedan 19 and Yiedan 19 can not be replaced.

Key words: Maize, Sister line, Sister-cross, Modified single cross, Original single cross, Albumin, Improved PAGE

引用本文

陈景堂, 池书敏, 马占元, 祝丽英, 刘志增, 孟义江, 宋占权, 魏俊杰. 玉米改良单交种的聚丙烯酰胺凝胶电泳分析[J]. 华北农学报, 2000, 15(4): 14-18. doi: 10.3321/j.issn:1000-7091.2000.04.004.

CHEN Jing tang, CHI Shu min, MA Zhan yuan, ZHU Li ying, LIU Zhi zeng, MENG Yi jiang, SONG Zhan quan, WEI Junjie. Analysis of Modified Single-cross of Maize with PAGE[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2000, 15(4): 14-18. doi: 10.3321/j.issn:1000-7091.2000.04.004.

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