华北农学报 ›› 1993, Vol. 8 ›› Issue (3): 92-97. doi: 10.3321/j.issn:1000-7091.1993.03.017

所属专题: 白菜

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通过游离小孢子培养方法获得小白菜三个变种的胚胎及植株

李岩, 刘凡, 曹鸣庆   

  1. 北京市农林科学院蔬菜研究中心, 北京 100081
  • 收稿日期:1993-04-15 出版日期:1993-09-28

Embryogenesis and Plant Regeneration from Isolated Microspore Cultures of Bras sic a campestris ssp. Chinensis

Li Yan, Liu Fan, Cao Mingqing   

  1. Beijing Vegetable Research Centre, Beijing 100081
  • Received:1993-04-15 Published:1993-09-28

摘要: 对普通小白菜、乌塌菜和菜三个小白菜变种的12个基因型进行了游离小孢子培养试验。从供体母株上选取2~4mm长的花蕾,机械分离小孢子并将其置于含NAA0.5mg/L、6BA0.05mg/L和蔗糖13%的NLN82液体培养基中薄层培养,先在33℃下暗培养24h,后移至25℃下继续暗培养,2周后有9个基因型形成球形期至子叶期胚。将成熟的小孢子胚移至B5无激素琼脂培养基上,继续发育为植株。

关键词: 小白菜, 游离小孢子培养, 胚胎发生

Abstract: Isolated microsporcs from three varieties of Brassica campestris ssp.chinensis: var.cammunis, var.rosularis and var.utilis, were cultured in modified NLN82 medium supplcmented with 13% (W/V) sucrose, benzyladcninc (BA) 0.05mg/L and naphthalcneaectic acid (NAA) 0.5mg/L in darkness at 33℃ for one day followed by cul-luring at 25℃ .After 2 weeks of culture, microspore-derived embryos ranging from the globulor to the colylcdonary stages were obtained in 9 out of 12 genotypes.Plantlcts were regenerated after transfer of the embryos to B5medium containing 2% sucrose without plant growth regulators.

Key words: Brassica campestrix ssp. chinensis, Isolated microsporc cultures, Embryogenesis

引用本文

李岩, 刘凡, 曹鸣庆. 通过游离小孢子培养方法获得小白菜三个变种的胚胎及植株[J]. 华北农学报, 1993, 8(3): 92-97. doi: 10.3321/j.issn:1000-7091.1993.03.017.

Li Yan, Liu Fan, Cao Mingqing. Embryogenesis and Plant Regeneration from Isolated Microspore Cultures of Bras sic a campestris ssp. Chinensis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1993, 8(3): 92-97. doi: 10.3321/j.issn:1000-7091.1993.03.017.

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