摘要： 采用水培的方法,研究0.1 mg/L和1 mg/L镉(Cd)处理时2个小白菜品种的Cd吸收和亚细胞分布及钙(Ca)、Cd互作关系。结果表明:Cd主要积累在小白菜的根中,杭州油冬儿地上部Cd含量显著高于沪青1号,但根中Cd含量相反。小白菜地上部亚细胞各组分中Cd含量随着营养液中Cd浓度增加而增加,Cd主要积累在细胞质组分中,其次为细胞壁组分,积累Cd量最少的是细胞器组分,两品种间存在差异,Cd在杭州油冬儿细胞壁组分的分配比率大于沪青1号。Ca也主要分布在细胞质组分中,0.1 mg/L Cd处理时,细胞质及细胞壁中的Ca、Cd为协同作用;1 mg/LCd处理时,二者为拮抗作用。

关键词: 小白菜, 镉, 钙, 亚细胞分布

Abstract: Cd uptake, subcellular distribution and interaction between Cd and Ca in pakchio were studied.The results showed that Cd was mainly kept in the roots of two cuhivars of pakchoi, and the concentration of Cd in shoot. Hangzhouyoudong was higher than that of∞.Huqing No.1, but the concentration of Cd in root was the opposite.The Cd concentrations of subcellular fraction increased with increasing Cd concentration in the growth medium.Cd was accumulated mainly in cytoplasm of shoots and this accumulation Was fairly independent of Cd concentration in nutrient solution.The lowest Cd concentration was found in organelles fractions of both Cd concentrations.There were differences between two cuhivars which the distribution rate of cell wall fraction in ca. Hangzhouyoudong was higher than that.Huqing No.1.Ca was mainly retained in the cytoplasm fraction.In cytoplasm and cell wall fractions. Cd and Ca had a synergistic effect at 0.1 mg/L Cd concentration.and had an antagonistic effect at 1.0 mg/L Cd con· centration.

Key words: Pakchoi, Cadmium, Calcium, Subcellular distribution

中图分类号: 

• S634.3