摘要： 为了探讨外源钙对提高羊草耐受性的作用，以内蒙古呼和浩特市和林格尔县的羊草种子为试验材料，采用无菌培养方法，研究了补加不同浓度CaCl₂对盐、碱、渗透、低温4种非生物胁迫下羊草幼苗生长及生理特性的影响。结果表明：150 mmol/L NaCl胁迫时羊草幼苗的生长受到显著抑制，当补加1 mmol/L CaCl₂后羊草种子发芽率显著提高，幼苗的根长、叶长与鲜质量增加。pH值8.5、300 mmol/L Mannitol胁迫时羊草幼苗的生长均会受到显著抑制，当补加1~20 mmol/L CaCl₂后未能有效缓解碱胁迫、渗透胁迫对羊草幼苗生长的影响。4℃胁迫时羊草幼苗的生长受到显著抑制，当补加20 mmol/L CaCl₂后羊草幼苗叶长、鲜质量增加，叶中丙二醛含量下降，超氧化物歧化酶、过氧化物酶、过氧化氢酶、谷胱甘肽还原酶活性增强，还原型抗坏血酸含量增加。综上所述，补加外源CaCl₂可以提高羊草幼苗的抗盐和抗低温能力，但是对抗碱和抗渗透胁迫能力没有改善。

关键词: 羊草, 钙, 非生物胁迫, 生长指标, 生理指标

Abstract: To explore to study the effect of exogenous calcium on the tolerance of Leymus chinensis, the seeds which collected from Horinger County, Hohhot City, Inner Mongolia were used as experimental materials, and the method of aseptic culture was used to study the effect of CaCl₂ on the growth and physiological characteristics of Leymus chinensis under four abiotic stresses of salt, alkali, osmotic and cold stress. The results showed that the growth of Leymus chinensis seedlings was significantly inhibited under 150 mmol/L NaCl stress. After adding 1 mmol/L CaCl₂ to the culture medium, the germination rate of seeds was significantly increased, and the root length, leaf length and fresh weight of seedlings were also increased. Under pH 8.5 or 300 mmol/L Mannitol stress, the growth of Leymus chinensis seedlings was both significantly inhibited. The effects of alkali stress and osmotic stress on the growth of Leymus chinensis seedlings could not be effectively alleviated by adding 1-20 mmol/L CaCl₂ to the culture medium respectively. Under 4℃ stress, the growth of Leymus chinensis seedlings was significantly inhibited. After adding 20 mmol/L CaCl₂ to the culture medium, the leaf length and fresh weight of seedlings increased significantly, the content of malondialdehyde(MDA) in leaves decreased, the activity of superoxide dismutase(SOD), peroxidase(POD), catalase(CAT) and glutathione reductase(GR) increased, and the content of reduced ascorbic acid(AsA) increased significantly. In conclusion, the addition of exogenous CaCl₂ could improve the salt and cold resistance of Leymus chinensis seedlings, but the ability to resist alkali and osmotic stress has not been improved.

Key words: Leymus chinensis, Calcium, Abiotic stress, Growth characteristics, Physiological characteristics

中图分类号: 

• Q945.78