镉污染土壤中甘薯镉元素的时空积累规律

doi:10.7668/hbnxb.20191671

摘要/Abstract

摘要： 为进一步明确甘薯镉元素的时空积累规律，探索甘薯薯肉低镉积累特性形成的原因，利用原子吸收光谱仪检测了在镉中度污染土壤和镉重度污染土壤生长条件下湘薯20号各组织器官镉分配与积累的时空差异，并用荧光定量PCR检测分析了与之对应的甘薯镉积累相关同源基因的表达差异。研究发现，镉污染土壤中甘薯的镉积累符合根>茎>叶的特性，当土壤镉浓度增加至0.6 mg/kg或2.0 mg/kg时，相对于阴性对照，各部位镉含量增加的倍数分别为3.05~8.75和6.25~26.89；各组织器官随生长时间延长植株镉积累量逐渐增加，地上部茎叶镉积累速率高于薯肉镉积累速率，且叶片中积累的镉不易发生二次转移，因而新叶中镉含量低于老叶。植物镉积累和转运相关的基因在甘薯基因组存在同源基因，且多数存在多个同源拷贝，虽然这些基因在一定程度上随着镉积累量的增加而被诱导表达，但是各拷贝之间的时空表达模式存在差异。相对于镉积累调控相关基因的表达，胁迫响应相关基因受镉胁迫诱导表达的趋势更为明显。镉污染土壤中甘薯的镉积累随着土壤镉浓度增加而增加，随着生长时间延长而增加。甘薯薯肉的镉积累规律较为特殊，薯肉镉积累量低于薯皮、茎、叶等部位，且随着生长时间的增加，薯肉镉积累速率也远低于地上部茎叶。植物镉积累和转运相关的基因在甘薯基因组存在同源基因，且多数存在多个同源拷贝，各拷贝之间的时空表达模式存在差异，推测这种表达差异与甘薯的镉积累特性有关。

关键词: 甘薯, 镉的积累, 同源基因, 时空表达模式

Abstract: In order to further clarify the spatial and temporal accumulation characteristics of cadmium in sweetpotato and to explore the causes of low cadmium accumulation in fleshed storage root,the temporal and spatial differences of cadmium accumulation in Xiangshu 20 were detected by atomic absorption spectrometry(AAS)in this study,and the expression difference of homologous genes related to cadmium accumulation in sweetpotato were analyzed by Fluorescence quantitative PCR.The results showed that the cadmium accumulation of sweetpotato followed the characteristics of root>stem>leaf. When plants were growth in the soil with 0.6 mg/kg cadmium,the increase rate of cadmium concentration in sweetpotato range from 3.05 to 8.75,and when plants growth in the soil with 2.0 mg/kg cadmium,the increase rate of cadmium concentration in sweetpotato range from 6.25 to 26.89. The accumulation rate of cadmium in shoots and leaves was higher than that in fleshed storage roots. The cadmium accumulated in leaves was not easy to transfer again,so cadmium concentration in new leaves was lower than that in old leaves. The homologous genes related to plant cadmium accumulation and transport existed in sweetpotato genome,and most of them had multiple homologous copies. Although these genes were induced to express with the increase of cadmium accumulation in plants,the spatiotemporal expression patterns of these genes were different. Compared with the expression pattern of genes related to cadmium accumulation,the expression of cadmium stress response related genes was been induced by cadmium accumulation more obvious. The cadmium accumulation in sweetpotato increased with the increase of soil cadmium concentration,and increased with the extension of growth time. The accumulation of cadmium in fleshed storage roots was lower than that in peels,stems and leaves,and the accumulation rate of cadmium in fleshed storage roots were much lower than that in shoots with the extension of growth time. The homologous genes related to plant cadmium accumulation and transport existed in sweetpotato genome,and most of them had multiple homologous copies. The spatiotemporal expression patterns of these genes were different,it was suggested that this expression difference was related to the cadmium accumulation characteristics of sweetpotato.

Key words: Sweetpotato, Cadmium accumulation, Homologous genes, Spatial and temporal expression patterns

中图分类号:

S143.7

张道微, 董芳, 张亚, 黄艳岚, 张超凡. 镉污染土壤中甘薯镉元素的时空积累规律[J]. 华北农学报, 2020, 35(S1): 289-298. doi: 10.7668/hbnxb.20191671.

ZHANG Daowei, DONG Fang, ZHANG Ya, HUANG Yanlan, ZHANG Chaofan. Spatial and Temporal Accumulation Characteristics of Cadmium in Sweetpotato Which Grown in Cadmium Contaminated Soil[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 289-298. doi: 10.7668/hbnxb.20191671.

http://www.hbnxb.net/CN/Y2020/V35/IS1/289

参考文献

[1] Jhanji S,Sadana U S. Genotypic variation in partitioning of dry matter and manganese between source and sink organs of rice under manganese stress[J]. Plant Cell Reports,2014,33(8):1227-1238. doi:10.1007/s00299-014-1611-x.
[2] Jhanji S,Sadana U S,Shankar A,Shukla A K. Manganese influx and its utilization efficiency in wheat[J]. Indian Journal of Experimental Biology,2014,52(6):650-657.
[3] Li L Z, Tu C, Wu L H, Peijnenburg W J G M, Ebbs S, Luo Y M.Pathways of root uptake and membrane transport of Cd²⁺ in the zinc/cadmium hyperaccumulating plant Sedum plumbizincicola[J]. Environmental Toxicology and Chemistry,2017,36(4):1038-1046. doi:10.1002/etc.3625.
[4] Song Y,Jin L,Wang X J. Cadmium absorption and transportation pathways in plants[J]. International Journal of Phytoremediation,2017,19(2):133-141. doi:10.1080/15226514.2016.1207598.
[5] 刘意章,肖唐付,熊燕,宁增平,双燕,李航,马良,陈海燕.西南高镉地质背景区农田土壤与农作物的重金属富集特征[J].环境科学,2019,40(6):2877-2884. doi:10.19303/j.issn.1008-0384.2019.03.014.
Liu Y Z,Xiao T F,Xiong Y,Ning Z P,Shuang Y,Li H,Ma L,Chen H Y.Accumulation of heavy metals in agricultural soils and crops from an area with a high geochemical background of cadmium,Southwestern China[J]. Environmental Science, 2019,40(6):2877-2884.
[6] Liu H W,Wang H Y,Ma Y B,Wang H H,Shi Y. Role of transpiration and metabolism in translocation and accumulation of cadmium in tobacco plants(Nicotiana tabacum L.)[J]. Chemosphere,2016,144:1960-1965. doi:10.1016/j.chemosphere.2015.10.093.
[7] Fu X P,Dou C M,Chen Y X,Chen X C,Shi J Y,Yu M G,Xu J. Subcellular distribution and chemical forms of cadmium in Phytolacca americana L.[J]. Journal of Hazardous Materials, 2011,186(1):103-107. doi:10.1016/j.jhazmat.2010.10.122.
[8] Chen X H,Ouyang Y N,Fan Y C,Qiu B Y,Zhang G P,Zeng F R. The pathway of transmembrane cadmium influx via calcium-permeable channels and its spatial characteristics along rice root[J]. Journal of Experimental Botany,2018,69(21):5279-5291. doi:10.1093/jxb/ery293.
[9] Zhang J,Martinoia E L,Young S. Vacuolar transporters for cadmium and arsenic in plants and their applications in phytoremediation and crop development[J]. Plant Cell Physiololgy,2018,59(7):1317-1325.doi:10.1093/pcp/pcy006.
[10] Parvaiz A,Maryam S,Nazir A B,Mohd R W,Alvina G K,Lam-Son P T. Alleviation of cadmium toxicity in Brassica juncea L.(Czern. & Coss.)by calcium application involves various physiological and biochemical strategies[J]. PLoS One,2015,10(1):e0114571. doi:10.1371/journal.pone.0114571.
[11] Stephan C. Safer food through plant science:reducing toxic element accumulation in crops[J]. Journal of Experimental Botany,2019,70(20):5537-5557. doi:10.1093/jxb/erz366.
[12] Pittman J K. Managing the manganese:molecular mechanisms of manganese transport and homeostasis[J]. The New Phytologist,2005,167(3):733-742. doi:10.1111/j.1469-8137.2005.01453.x.
[13] Wu F B,Dong J,Qian Q Q,Zhang G P. Subcellular distribution and chemical form of Cd and Cd-Zn interaction in different barley genotypes[J]. Chemosphere,2005,60(10):1437-1446. doi:10.1016/j.chemosphere.2005.01.071.
[14] Xin J L,Huang B F,Dai H W,Liu A Q,Zhou W J,Liao K B. Characterization of cadmium uptake,translocation,and distribution in young seedlings of two hot pepper cultivars that differ in fruit cadmium concentration[J]. Environmental Science and Pollution Research International,2014,21(12):7449-7456.doi:10.1007/s11356-014-2691-4.
[15] Mélanie M,Jérôme C,Antoine G,Pascaline A,Nathalie L,Alain V,Pierre R. AtHMA3,a P1B-ATPase allowing Cd/Zn/Co/Pb vacuolar storage in Arabidopsis[J]. Plant Physiology, 2009,149(2):894-904.doi:10.1104/pp.108.130294.
[16] Zhao J,Toshiro S,Mei H,Guo Y Q,Cheng N H,Kendal D H. Interaction between Arabidopsis Ca²⁺/H⁺ exchangers CAX1 and CAX3[J]. The Journal of Biological Chemistry Vol,2009,284(7):4605-4615. doi:10.1074/jbc.M804462200.
[17] Santiago A,Rémy C,Carine A,Léon D,Frédéric D,David C,Loren C,Jean-François B,Stéphane M,Catherine C. Intracellular distribution of manganese by the trans-golgi network transporter NRAMP2 is critical for photosynthesis and cellular redox homeostasis[J]. The Plant Cell,2017,29(12):3068-3084.doi:10.1105/tpc.17.00578.
[18] Sheng Y B,Yan X X,Huang Y,Han Y Y,Zhang C,Ren Y B,Fan T T,Xiao F M,Liu Y S,Cao S Q. The WRKY transcription factor,WRKY13,activates PDR8 expression to positively regulate cadmium tolerance in Arabidopsis[J]. Plant Cell and Environment,2019,42(3):891-903. doi:10.1111/pce.13457.
[19] Kühnlenz T,Holger S,Shimpei U,Stephan C. Arabidopsis thaliana phytochelatin synthase 2 is constitutively active in vivo and can rescue the growth defect of the PCS1-deficient cad1-3 mutant on Cd-contaminated soil[J]. Journal of Experimental Botany,2014,65(15):4241-4253. doi:10.1093/jxb/eru195.
[20] Liu X S,Feng F J,Zhang B Q,Wang M Q,Cao H W,Justice K R,Chen X,Yang Z M. OsZIP1 functions as a metal efflux transporter limiting excess zinc,copper and cadmium accumulation in rice[J]. BMC Plant Biology, 2019,19:283. doi:10.1186/s12870-019-1899-3.
[21] Gu L J,Zhao M L,Ge M,Zhu S W,Cheng B J,Li X Y. Transcriptome analysis reveals comprehensive responses to cadmium stress in maize inoculated with arbuscular mycorrhizal fungi[J]. Ecotoxicology and Environmental Safety,2019,30(186):109744. doi:10.1016/j.ecoenv.2019.109744.
[22] Seiichiro H,Daigo S,Hitomi F. Toxicometallomics of cadmium,manganese and arsenic with special reference to the roles of metal transporters[J]. Toxicological Research,2019,35(4):311-317. doi:10.5487/TR.2019.35.4.311.
[23] Ramin B,Mahsa M,Mohammad R B. Genotypic variation for cadmium tolerance in common bean(Phaseolus vulgaris L.)[J]. Ecotoxicology and Environmental Safety,2020,1(190):110178. doi:10.1016/j.ecoenv.2020.110178.
[24] Kocadal K,Alkas F B,Battal D,Saygi S. Cellular pathologies and genotoxic effects arising secondary to heavy metal exposure:A review[J]. Human & Experimental Toxicology, 2020,39(1):3-13.doi:10.1177/0960327119874439.
[25] 张杰.超积累植物东南景天Cd耐性和积累的分子机制[D].杭州:浙江大学,2015.
Zhang J.Molecular mechanisms of Cd tolerance and accumulation in metal hyperaccumulator Sedum alfredii[D].Hangzhou:Zhejiang University,2015.
[26] 胡玉龙,李雪华,赵苹艺,丁楚楚,徐甜甜,孙存华.镉胁迫对甘薯苗生理生化指标的影响[J].湖北农业科学,2015,54(4):858-861. doi:10.14088/j.cnki.issn0439-8114.2015.04.021.
Hu Y L,Li H,Zhao P Y,Ding C C,Xu T T,Sun C H.Effects of cadmium stress on physiologic and biochemical characteristics of sweet potato[J]. Hubei Agricultural Science,2015,54(4):858-861.
[27] Cheng S F,Huang C Y. Accumulation of cadmium uptake from soilin the edible root of root vegetables[J]. Journal of Environmental Management,2007,17:137.
[28] 刘兰英,吕新,陈丽华,黄薇,涂杰峰,余华,上官亮,谢亚兴.土壤镉胁迫对甘薯品质和镉、锌吸收的影响[J].福建农业学报,2019,34(3):344-351. doi:10.19303/j.issn.1008-0384.2019.03.014.
Liu L Y,Lü X,Chen L H,Huang W,Tu J F,Yu H,Shangguan L,Xie Y X. Cd and Zn uptakes and quality of sweetpotatoes under Cd-stress[J]. Fujian Journal of Agricultural Science,2019,34(3):344-351.
[29] Huang B F,Xin J L,Dai H W,Zhou W J,Peng L J. Identification of low-Cd cultivars of sweet potato(Ipomoea batatas (L.)Lam.)after growing on Cd-contaminated soil:uptake and partitioning to the edible roots[J]. Environmental Science and Pollution Research International,2015,22(15):11813-11821.doi:10.1007/s11356-015-4449-z.
[30] Xin J L,Dai H W,Huang B F. Assessing the roles of roots and shoots in the accumulation of cadmium in two sweet potato cultivars using split-root and reciprocal grafting systems[J]. Plant and Soil,2016,412(1-2):413-424. doi:10.1007/s11104-016-3079-7.
[31] Huang B F,Dai H W,Zhou W J,Peng L J,Li M Z,Wan R J,He W T. Characteristics of Cd accumulation and distribution in two sweet potato cultivars[J]. International Journal of Phytoremediation,2019,21(4):391-398. doi:10.1080/15226514.2018.1524846.
[32] Zhang D W, Dong F, Zhang Y, Huang Y L, Zhang C F. Mechanisms of low cadmium accumulation in storage root of sweetpotato(Ipomoea batatas L.)[J].Journal of Plant Physiology,2020,254:153262.doi:10.1016/j.jplph.2020.153262.
[33] Tang J,Wang S Q,Hu K D,Huang Z Q,Li Y H,Han Z,Chen X Y,Hu L Y,Yao G F,Zhang H. Antioxidative capacity is highly associated with the storage property of tuberous roots in different sweetpotato cultivars[J]. Scientific Reports,2019,9(1):11141.doi:10.1038/s41598-019-47604-8.
[34] Livak K J,Schmittgen T D. Analysis of relative gene expression data using Real-time quantitative PCR and the 2^-ΔΔC_T Method[J]. Methods, 2001,25(4):402-408. doi:10.1006/meth.2001.1262.
[35] Kim D Y,Lucien B,Masayoshi M,Enrico M,Lee Y S. The ABC transporter AtPDR8 is a cadmium extrusion pump conferring heavy metal resistance[J]. Plant Journal,2007,50(2):207-218. doi:10.1111/j.1365-313X.2007.03044.x.
[36] Ryuichi T,Yasuhiro I,Hiromi N,Naoko K N. Role of the iron transporter OsNRAMP1 in cadmium uptake and accumulation in rice[J]. Plant Signaling & Behavior,2011,6(11):1813-1816.doi:10.4161/psb.6.11.17587.
[37] Wu Q Y,Toshiro S,Kimberly A W,Han J S,Chang K K,Kendal D H,Park S. Expression of an Arabidopsis Ca²⁺/H⁺ antiporter CAX1 variant in petunia enhances cadmium tolerance and accumulation[J]. Journal of Plant Physiology,2011,15,168(2):167-73. doi:10.1016/j.jplph.2010.06.005.
[38] Shen J J,Qiao Z J,Xing T J,Zhang L P,Liang Y L,Jin Z P,Yang G D,Wang R,Pei Y X. Cadmium toxicity is alleviated by AtLCD and AtDCD in Escherichia coli[J]. Journal of Applied Microbiology,2012,113(5):1130-1138. doi:10.1111/j.1365-2672.2012.05408.x.
[39] Natasha D,Surajit B,Mrinal K M. Enhanced cadmium accumulation and tolerance in transgenic tobacco overexpressing rice metal tolerance protein gene OsMTP1 is promising for phytoremediation[J]. Plant Physiology and Biochemistry, 2016,105:297-309.doi:10.1016/j.plaphy.2016.04.049.
[40] Magdalena M,Anna K,Anna P,Ewa M D,Ewelina P,Arnold G,Sophie F. Two metal-tolerance proteins,MTP1 and MTP4,are involved in Zn homeostasis and Cd sequestration in cucumber cells[J]. Journal of Experimental Botany, 2015,66(3):1001-1015. doi:10.1093/jxb/eru459.
[41] Chen J,Sylvie L,Petr O,Azam N V,Saman A P,Cristina V,Jose L R,Wolf B F,Seung Y R. Uncovering Arabidopsis membrane protein interactome enriched in transporters using mating-based split ubiquitin assays and classification models[J]. Frontiers in Plant Science, 2012,21(3):124. doi:10.3389/fpls.2012.00124.
[42] Wu Z Y,Liang F,Hong B M,Young Y C,Michael R S,Jeffrey F H,Heven S. An endoplasmic reticulum-bound Ca(2+)/Mn(2+)pump,ECA1,supports plant growth and confers tolerance to Mn(2+)stress[J]. Plant Physiology, 2002,130(1):128-137. doi:10.1104/pp.004440.
[43] Chen J,Yang L B,Gu J,Bai X Y,Ren Y B,Fan T T,Han Y,Jiang L,Xiao F M,Liu Y S,Cao S Q. MAN3 gene regulates cadmium tolerance through the glutathione-dependent pathway in Arabidopsis thaliana[J].The New Phytologist,2015,205(2):570-582.doi:10.1111/nph.13101.

选择文件类型/文献管理软件名称

选择包含的内容