华北农学报 ›› 2020, Vol. 35 ›› Issue (S1): 289-298. doi: 10.7668/hbnxb.20191671

所属专题: 薯类作物 土壤肥料

• 资源环境·植物保护 • 上一篇    下一篇

镉污染土壤中甘薯镉元素的时空积累规律

张道微, 董芳, 张亚, 黄艳岚, 张超凡   

  1. 湖南省作物研究所, 湖南 长沙 410125
  • 收稿日期:2020-08-18 出版日期:2020-12-28
  • 通讯作者: 张超凡(1962-),男,湖南邵阳人,研究员,博士,主要从事甘薯遗传育种、栽培研究。
  • 作者简介:张道微(1987-),男,湖南武冈人,硕士,助理研究员,主要从事甘薯育种研究。
  • 基金资助:
    国家甘薯产业技术体系(CARS-11-C-16);食用作物安全性评价(湘农财指,2019年[33]号);湖南省甘薯工程技术研究中心项目(2018TP2032)

Spatial and Temporal Accumulation Characteristics of Cadmium in Sweetpotato Which Grown in Cadmium Contaminated Soil

ZHANG Daowei, DONG Fang, ZHANG Ya, HUANG Yanlan, ZHANG Chaofan   

  1. Crop Research Institute of Hunan Province, Changsha 410125, China
  • Received:2020-08-18 Published:2020-12-28

摘要: 为进一步明确甘薯镉元素的时空积累规律,探索甘薯薯肉低镉积累特性形成的原因,利用原子吸收光谱仪检测了在镉中度污染土壤和镉重度污染土壤生长条件下湘薯20号各组织器官镉分配与积累的时空差异,并用荧光定量PCR检测分析了与之对应的甘薯镉积累相关同源基因的表达差异。研究发现,镉污染土壤中甘薯的镉积累符合根>茎>叶的特性,当土壤镉浓度增加至0.6 mg/kg或2.0 mg/kg时,相对于阴性对照,各部位镉含量增加的倍数分别为3.05~8.75和6.25~26.89;各组织器官随生长时间延长植株镉积累量逐渐增加,地上部茎叶镉积累速率高于薯肉镉积累速率,且叶片中积累的镉不易发生二次转移,因而新叶中镉含量低于老叶。植物镉积累和转运相关的基因在甘薯基因组存在同源基因,且多数存在多个同源拷贝,虽然这些基因在一定程度上随着镉积累量的增加而被诱导表达,但是各拷贝之间的时空表达模式存在差异。相对于镉积累调控相关基因的表达,胁迫响应相关基因受镉胁迫诱导表达的趋势更为明显。镉污染土壤中甘薯的镉积累随着土壤镉浓度增加而增加,随着生长时间延长而增加。甘薯薯肉的镉积累规律较为特殊,薯肉镉积累量低于薯皮、茎、叶等部位,且随着生长时间的增加,薯肉镉积累速率也远低于地上部茎叶。植物镉积累和转运相关的基因在甘薯基因组存在同源基因,且多数存在多个同源拷贝,各拷贝之间的时空表达模式存在差异,推测这种表达差异与甘薯的镉积累特性有关。

关键词: 甘薯, 镉的积累, 同源基因, 时空表达模式

Abstract: In order to further clarify the spatial and temporal accumulation characteristics of cadmium in sweetpotato and to explore the causes of low cadmium accumulation in fleshed storage root,the temporal and spatial differences of cadmium accumulation in Xiangshu 20 were detected by atomic absorption spectrometry(AAS)in this study,and the expression difference of homologous genes related to cadmium accumulation in sweetpotato were analyzed by Fluorescence quantitative PCR.The results showed that the cadmium accumulation of sweetpotato followed the characteristics of root>stem>leaf. When plants were growth in the soil with 0.6 mg/kg cadmium,the increase rate of cadmium concentration in sweetpotato range from 3.05 to 8.75,and when plants growth in the soil with 2.0 mg/kg cadmium,the increase rate of cadmium concentration in sweetpotato range from 6.25 to 26.89. The accumulation rate of cadmium in shoots and leaves was higher than that in fleshed storage roots. The cadmium accumulated in leaves was not easy to transfer again,so cadmium concentration in new leaves was lower than that in old leaves. The homologous genes related to plant cadmium accumulation and transport existed in sweetpotato genome,and most of them had multiple homologous copies. Although these genes were induced to express with the increase of cadmium accumulation in plants,the spatiotemporal expression patterns of these genes were different. Compared with the expression pattern of genes related to cadmium accumulation,the expression of cadmium stress response related genes was been induced by cadmium accumulation more obvious. The cadmium accumulation in sweetpotato increased with the increase of soil cadmium concentration,and increased with the extension of growth time. The accumulation of cadmium in fleshed storage roots was lower than that in peels,stems and leaves,and the accumulation rate of cadmium in fleshed storage roots were much lower than that in shoots with the extension of growth time. The homologous genes related to plant cadmium accumulation and transport existed in sweetpotato genome,and most of them had multiple homologous copies. The spatiotemporal expression patterns of these genes were different,it was suggested that this expression difference was related to the cadmium accumulation characteristics of sweetpotato.

Key words: Sweetpotato, Cadmium accumulation, Homologous genes, Spatial and temporal expression patterns

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引用本文

张道微, 董芳, 张亚, 黄艳岚, 张超凡. 镉污染土壤中甘薯镉元素的时空积累规律[J]. 华北农学报, 2020, 35(S1): 289-298. doi: 10.7668/hbnxb.20191671.

ZHANG Daowei, DONG Fang, ZHANG Ya, HUANG Yanlan, ZHANG Chaofan. Spatial and Temporal Accumulation Characteristics of Cadmium in Sweetpotato Which Grown in Cadmium Contaminated Soil[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 289-298. doi: 10.7668/hbnxb.20191671.

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