华北农学报

所属专题: 白菜

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小白菜小孢子胚状体诱导影响因素研究

王超楠1,2, 冯辉1,2, 姜凤英2, 刘如娥1,2, 周英1,2   

  1. 1. 沈阳农业大学国艺学院, 辽宁沈阳?? 110161;
    2. 辽宁省农业科学院花卉研究所, 辽宁沈阳?? 110161
  • 收稿日期:2007-03-10 出版日期:2007-12-30
  • 通讯作者: 冯辉(1961-) , 男, 辽宁沈阳人, 教授, 主要从事蔬菜育种研究。
  • 作者简介:王超楠(1982-) , 女, 天津人, 硕士研究生, 主要从事细胞遗传学方面研究
  • 基金资助:
    国家自然科学基金(30671414);国家863项目(20060110Z1072)

Embryogenesis from IsolatedMicrospore Culture of Chinese Cabbage ( Brassicacampestris L. ssp. chinensis L. )

WANGChao-nan1,2, FENGHui1,2, JIANGFeng-ying2, LIURu-e1,2, ZHOUYing1,2   

  1. 1. College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China;
    2. Institute of Floriculture,Liaoning Academy of Agricultural Science, Shenyang 110161, China
  • Received:2007-03-10 Published:2007-12-30

摘要: 以10个小白菜杂交种为试材,采用游离小孢子培养方法,研究影响小孢子胚状体形成因素的结果表明,不同品种胚状体的诱导率不同;NLN培养基中大量元素减半有利于小孢子胚诱导,其诱导率达到100%;培养基中添加0.05mg/L的6-BA和0.2mg/L的NAA可以提高胚状体诱导率;活性炭和琼脂糖对诱导胚状体发生和发育有促进作用,可使子叶胚的比例达90%以上;花蕾经4℃低温预处理24h后的成胚率显著提高。

关键词: 小白菜, 游离小孢子培养, 胚状体

Abstract: Isolated microspore culture was carried out by ten F1 hybrids of Chinese cabbage ( Brassica campestris L.ssp. chinensis L. ) . It was found that the effect of genotypes on embryoid induction was remarkable. Reducing the contentof major salts of NLN medium by half can raise the embryoid induction rate up to 100% ; the suitable concentrat ion of 6BA and NAA can remarkably enhance microspore embryogenesis.Agarose and active carbon could not only promote em-bryogenesis, but also increase the proport ion of cotyledon-shaped embryoids, the cotyledon-shaped embryoid rate was over90% . 4℃pretreatment of bud for 24 h significantly enhanced frequency of embryoids.

Key words: Chinese cabbage, Isolated microspore culture, Embryoid

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引用本文

WANGChao-nan, FENGHui, JIANGFeng-ying, LIURu-e, ZHOUYing. Embryogenesis from IsolatedMicrospore Culture of Chinese Cabbage ( Brassicacampestris L. ssp. chinensis L. )[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2007.S1.050.

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               http://www.hbnxb.net/CN/Y2007/V22/IS1/204