摘要： 以2个小白菜品种为材料，通过在NLN培养基中添加不同浓度活性炭，研究其对游离小孢子培养的影响。结果表明：在NLN-13培养基中添加活性炭(0.5 g/L)，有利于小孢子胚的诱导和形成，供试材料(N，N8)添加活性炭处理胚诱导率较未添加活性炭的处理分别提高19，66.5倍。采用添加活性炭(0.5 g/L)的NLN培养基对2份小白菜品种进行游离小孢子培养，有20份材料诱导出胚，培养成功率为83.3%。表明添加活性炭的培养基对小白菜游离小孢子培养有较好的效果。不同基因型间胚诱导率差别较大，每花蕾诱导胚数为 0～86.7个。依据胚诱导率的高低可将其分为极易诱导、易诱导、难诱导和不能诱导出胚类。B5+6-BA 0.2 mg/L+NAA 0.02 mg/L+活性炭0.5 g/L +3%蔗糖+1%琼脂培养基有利于幼胚长成植株。

关键词: 小白菜, 游离小孢子培养, 小孢子胚, 诱导率, 活性炭

Abstract: Fluency of activated charcoal on isolated microspore culture in Chinese cabbage was studied.The results showed that NLN-13 liquid medium with activated charcoal(0.5 g/L)was obviously better in frequency of microspore-derived embryos and frequency of regenerated plants.Frequency of microspore-derived embryos in N4 and N8 was increased 19 and 66.5 times.Isolated microspores from 24 varieties of Chinese cabbage were cultured in NLN-13 liquid medium with activated charcoal(0.5 g/L),20 varieties were produced microspore derived embryos.Embryo yield per bud(frequency microspore-derived embryos)was from 0.4 to 86.7.Microspore-derived embryos on B₅medium with 6-BA 0.2mg/L and NAA 0.02 mg/L and activated charcoal 0.5 g/L and 3% sucrose and 1%agar is good to regenerated plants.

Key words: Chinese cabbage, Isolated microspore culture, Microspore-derived embryos, Frequency, Activated charcoal

中图分类号: 

• S634.303.4