摘要： 以津稻291/IRBB60籼粳杂交F₁、反交F₁及其亲本的花药为外植体，对诱导和分化培养基、激素配比及有机附加物和高温预培养时间等影响花药培养效果的因素进行研究。结果表明，在SK3基本培养基中添加2 mg/L 2，4-D，1 mg/L NAA和1 mg/L KT比只添加2 mg/L 2，4-D的愈伤诱导率高0.67～2.78个百分点；在诱导培养基中添加水解酪蛋白能明显提高正交F₁的愈伤诱导率；MS+1 mg/L NAA+2 mg/L 6-BA+0.5 mg/L KT分化培养基得到了较高的绿苗分化率；正交F₁的愈伤诱导率、绿苗分化率和花培力显著高于反交F₁；30℃高温预培养36 h，正交F₁花培力达到最高值。

关键词: 水稻, 花药培养, 愈伤诱导率, 绿苗分化率

Abstract: The anther of Jindao291/IRBB60 and their parents were used to induce the calli in the different medium supplemented with different concentrations and combinations of phytohormones and organic nutriments and different high temperature pre-culture treatments. The results showed that the frequency of the callus induction in the SK3 medium supplemented with 2 mg/L 2, 4-D, 1 mg/L NAA and 1 mg/L KT was 0.67%-2.78% higher than that with 2 mg/L 2, 4-D. The induction rate could be increased in this medium added with hydrolyzed casein. It could be obtained the highest plant regeneration rate in the MS medium added with NAA (1 mg/L), 6-BA (2 mg/L) and KT (0.5 mg/L). The frequency of the callus induction and green plantlet differentiation and anther culture ability of the cross F₁ is significantly higher than that reverse F₁. Anther culture ability may be the highest when anthers were pre-cultured with 30℃ for 36 h.

Key words: Rice, Anther culture, Callus induction rate, Frequency of green plant regeneration

中图分类号: 

• S511.035.3