Molecular Cloning and Expression Pattern of the StHY5 Gene in Potato

doi:10.7668/hbnxb.20196549

Abstract

Abstract:

To clarify the basic characteristics and tissue expression pattern of the light signal transduction core bZIP transcription factor StHY5 gene,using potato cultivar Désirée as the experimental material,the full-length CDS sequence of the StHY5 gene was cloned.The sequence characteristics and evolutionary relationship were clarified by bioinformatics analysis.The expression patterns in different potato tissues were analyzed by Quantitative Real-time PCR(qRT-PCR).The results showed that the StHY5 protein consisted of 158 amino acids,with a molecular weight of 17.32 ku and a theoretical isoelectric point of 9.78.It contained one basic leucine zipper(bZIP)domain.The secondary structure of StHY5 was composed of 44.3% α-helices and 55.7% random coils,while the tertiary structure was dominated by α-helices,using the chicken CREB3 bZIP protein as the template.Phylogenetic tree analysis revealed that StHY5 clusters with HY5 proteins from Solanaceae species,showing the closest genetic relationship to CaHY5 in capsicum.qRT-PCR quantification demonstrated that StHY5 was expressed in all tested tissues with significant expression differences,among them the highest expression in stolons,and the lowest expression in tubers.This study clarifies the sequence characteristics and tissue expression pattern of the potato StHY5 gene,providing an experimental foundation for subsequent functional verification and pathway analysis in light signal regulation.

Key words: Potato, StHY5, Gene cloning, Expression pattern analysis, Bioinformatics analysis, Light signaling pathway

CLC Number:

Q785转化及克隆

QIN Ruihan, YI Rong, FENG Jiajia, LIANG Jiayi, BAI Jianhui, FAN Mingshou, JIA Liguo. Molecular Cloning and Expression Pattern of the StHY5 Gene in Potato[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(4): 44-50. doi: 10.7668/hbnxb.20196549.

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Figures/Tables 7

Fig.1 Electrophoresis of the StHY5 gene sequence M.DL2000 DNA Marker;1.StHY5 gene amplification product.

Fig.2 Electrophoresis of monoclonal bacterial suspension PCR M.DL2000 DNA Marker;1,2.StHY5 gene amplification product.

Fig.3 Bioinformatics analysis of StHY5 A.Predicted conserved domains of the StHY5 protein;B.Hydrophobicity analysis of the StHY5 protein;C.Prediction of StHY5 protein transmembrane domains.

Fig.4 Structural analysis of the StHY5 protein in potato A.Secondary structure prediction of the StHY5 protein;B.Tertiary structure prediction of the StHY5 protein.

Fig.5 Phylogenetic tree of plant HY5 proteins StHY5.Solanum tuberosum;CaHY5.Capsicum annuum;SlHY5.Solanum lycopersicum;NtHY5.Nicotiana tabacum;SmHY5.Solanum melongena;AtHY5.Arabidopsis thaliana;VvHY5.Vitis vinifera;MdHY5.Malus domestica;AhHY5.Arachis hypogaea;CsHY5.Citrus sinensis;OsHY5.Oryza sativa;ZmHY5.Zea mays;TaHY5.Triticum aestivum.The same as Fig.6.

Fig.6 Multiple sequence alignment of the plant HY5 protein

Fig.7 Expression patterns of the StHY5 gene in different tissues Different letters indicate significant differences between groups(P<0.05).

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