Isolation,Identification and Pathogenicity Determination of Potato Common Scab Streptomyces in Double-cropping Cultivation Region of Hebei Province

doi:10.7668/hbnxb.20195796

Abstract

Abstract:

In order to clarify the types of pathogens causing potato common scab in double-cropping cultivation region of Hebei Province,we isolated different types of symptoms tubers which diseased with potato common scab.Morphological identification,PCR molecular identification,and detection of pathogenic genes textAB,necl,and tomA were performed on the isolated strains.The pathogenicity of the strains containing pathogenic genes was further validated using potato chip and radish seedling methods.Finally, the isolated strains were identified at the species level by 16S rDNA sequencing, and a corresponding phylogenetic tree was constructed.The results showed that a total of 6 strains of Streptomyces were isolated from 19 diseased potatoes with different symptom.Through 16S rDNA sequence and amplification analysis,SJZ-1 and CL-1 were identified as S.scabies,SJZ-2 was identified as S.anthocyanicus,CL-2 was identified as S.albogriseolus,SFC was identified as S.thermocarboxydus,QY was identified as S.europaeiscabiei.Strain SJZ-1 and CL-1 contained three pathogenic genes:textAB,necl and tomA.Strain QY only contained the tomA gene.Strain SJZ-2,CL-2 and SFC did not contain any of these three pathogenic genes.The potato chip method showed that QY and SJZ-1 could colonize and produce necrotic spots on small potato chips,the radish seedling method showed that SJZ-1 and QY could inhibit the growth of radish seedlings,while SJZ-2,CL-2,and SFC had no significant pathogenicity in potato chip and radish seedling.This study have preliminary identified the types and composition of potato scab pathogen in double-cropping cultivation region of Hebei Province,and providing a theoretical basis for targeted prevention and potato common scab.

Key words: Potato common scab, Streptomyces app., Pathogenicity

CLC Number:

LI Yifan, HAN Dongbing, QI Ledan, TIAN Mengjun, SONG Xue, ZHANG Yuhan, ZHU Jiehua, ZHAO Dongmei, YANG Zhihui. Isolation,Identification and Pathogenicity Determination of Potato Common Scab Streptomyces in Double-cropping Cultivation Region of Hebei Province[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(S1): 270-276. doi: 10.7668/hbnxb.20195796.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20195796

http://www.hbnxb.net/EN/Y2025/V40/IS1/270

Figures/Tables 9

References 27

[1]	夏善勇, 盛万民. 我国马铃薯疮痂病及其防治研究进展[J]. 植物保护, 2022, 48(1):7-16,28.doi:10.16688/j.zwbh.2020505.
	Xia S Y, Sheng W M. Research progress on potato common scab disease in China and its control measures[J]. Plant Protection, 2022, 48(1):7-16,28.
[2]	田丹丹. 黑龙江省马铃薯疮痂病生防菌的筛选、鉴定及发酵优化[D]. 泰安: 山东农业大学, 2021.doi:10.27277/d.cnki.gsdnu.2021.000864.
	Tian D D. Screening,identification and fermentation optimization of biocontrol bacteria against potato scab in Heilongjiang Province[D]. Taian: Shandong Agricultural University, 2021.
[3]	Bukhalid R A, Loria R. Cloning and expression of a gene from Streptomyces scabies encoding a putative pathogenicity factor[J]. Journal of Bacteriology, 1997, 179(24):7776-7783.doi:10.1128/jb.179.24.7776-7783.1997.
[4]	史文宠. 马铃薯疮痂病发生的土壤微生态机制研究[D]. 泰安: 山东农业大学, 2022.doi:10.27277/d.cnki.gsdnu.2022.000055.
	Shi W C. Soil microbiome mechanisms for potatocommon scab development[D]. Taian: Shandong Agricultural University, 2022.
[5]	Fyans J K, Bown L, Bignell D R D. Isolation and characterization of plant-pathogenic Streptomyces Species associated with common scab-infected potato tubers in newfoundland[J]. Phytopathology, 2016, 106(2):123-131.doi:10.1094/phyto-05-15-0125-r. URL
[6]	Park D H, Kim J S, Kwon S W, Wilson C, Yu Y M, Hur J H, Lim C K. Streptomyces luridiscabiei sp.nov,Streptomyces puniciscabiei sp.nov and Streptomyces niveiscabiei sp.nov,which cause potato common scab disease in Korea[J]. International Journal of Systematic and Evolutionary Microbiology, 2003, 53(6):2049-2054.doi:10.1099/ijs.0.02629-0.
[7]	Loria R, Bukhalid R A, Fry B A, King R R. Plant pathogenicity in the genus Streptomyces[J]. Plant Disease, 1997, 81(8):836-846.doi:10.1094/pdis.1997.81.8.836. URL
[8]	Miyajima K, Tanaka F, Takeuchi T, Kuninaga S. Streptomyces turgidiscabies sp.nov[J]. International Journal of Systematic Bacteriology, 1998, 48(2):495-502.doi:10.1099/00207713-48-2-495. URL
[9]	夏善勇, 牛志敏, 李庆全, 张丽娟, 盛万民. 马铃薯疮痂病菌及防控手段研究进展[J]. 中国瓜菜, 2022, 35(8):12-17.doi:10.16861/j.cnki.zggc.2022.0182.
	Xia S Y, Niu Z M, Li Q Q, Zhang L J, Sheng W M. Disease caused by Streptomyces spp.and its control measures[J]. China Cucurbits and Vegetables, 2022, 35(8):12-17.
[10]	张萌, 刘伯, 于秀梅, 赵伟全, 刘大群. 中国马铃薯疮痂病菌生物学特性分析[J]. 中国农业科学, 2010, 43(12):2603-2610.doi:10.3864/j.issn.0578-1752.2010.12.028.
	Zhang M, Liu B, Yu X M, Zhao W Q, Liu D Q. Biological characteristics analysis of the pathogens caused potato common scab in China[J]. Scientia Agricultura Sinica, 2010, 43(12):2603-2610.
[11]	徐李娟, 陈勇, 王则玉, 王博, 艾尼江·尔斯满, 郭瑞, 李克梅, 宋素琴. 新疆拜城县马铃薯疮痂病病原菌的分离鉴定及生物学特性分析[J]. 新疆农业科学, 2023, 60(9):2258-2265.doi:10.6048/j.issn.1001-4330.2023.09.021.
	Xu L J, Chen Y, Wang Z Y, Wang B, Ainijiang E S M, Guo R, Li K M, Song S Q. Isolation,identification and biological characteristics of potato scab pathogen in Baicheng,Xinjiang[J]. Xinjiang Agricultural Sciences, 2023, 60(9):2258-2265.
[12]	殷修鲁. 我国马铃薯疮痂病病原菌多样性及主要致病类型分析[D]. 泰安: 山东农业大学, 2018.doi:10.7666/d.D01436659.
	Yin X L. Diversity and main pathogenic types of pathogen isolates from potato scab in China[D]. Taian: Shandong Agricultural University, 2018.
[13]	王越, 曹春梅, 陈汉, 王晓娇, 余乾鹏, 李学洋, 张志凯, 胡柏耿. 乌兰察布地区马铃薯疮痂病新型病原菌种类及致病性鉴定[J]. 中国农学通报, 2024, 40(32):151-156.doi:10.11924/j.issn.1000-6850.casb2024-0170.
	Wang Y, Cao C M, Chen H, Wang X J, Yu Q P, Li X Y, Zhang Z K, Hu B G. Species and pathogenicity identification of Streptomyces species causing potato common scab in Ulanqab Area[J]. Chinese Agricultural Science Bulletin, 2024, 40(32):151-156.
[14]	杨梦平, 王瑞仙, 杜魏甫, 巩晨, 于德才, 赵伟全, 宋双伟, 张红骥. 云南省马铃薯疮痂病致病链霉菌种类组成研究[J]. 植物病理学报, 2018, 48(4):445-454.doi:10.13926/j.cnki.apps.000134.
	Yang M P, Wang R X, Du W F, Gong C, Yu D C, Zhao W Q, Song S W, Zhang H J. The pathogenic Streptom yces species causing potato scab in Yunnan Province[J]. Acta Phytopathologica Sinica, 2018, 48(4):445-454.
[15]	陈利达, 李磊, 谢学文, 石延霞, 柴阿丽, 李宝聚. 山东高密地区马铃薯疮痂病菌种类及致病性鉴定[J]. 华北农学报, 2020, 35(S1):347-354.doi:10.7668/hbnxb.20191183.
	Chen L D, Li L, Xie X W, Shi Y X, Chai A L, Li B J. Species and pathogenicity identification of Streptomyces species causing potato common scab in Gaomi City,Shandong Province[J]. Acta Agriculturae Boreali-Sinica, 2020, 35(S1):347-354.
[16]	张海颖, 郭凤柳, 许华民, 赵伟全, 刘大群. 河北省张北地区马铃薯疮痂病的病菌鉴定[J]. 江苏农业科学, 2014, 42(10):131-134.doi:10.15889/j.issn.1002-1302.2014.10.200.
	Zhang H Y, Guo F L, Xu H M, Zhao W Q, Liu D Q. Identification of the pathogen of potato scab in Zhangbei area of Hebei Province[J]. Jiangsu Agricultural Sciences, 2014, 42(10):131-134.
[17]	张淑青, 相丛超, 李东玉, 封志明, 樊建英. 2019年河北省二季作区马铃薯产业发展现状、存在问题及建议[C]. 中国甘肃定西: 2020.doi:10.26914/c.cnkihy.2020.018912.
	Zhang S Q, Xiang C C, Li D Y, Feng Z M, Fan J Y. Development status,existing problems and suggestions of potato industry in the second cropping area of Hebei Province in 2019[C]. Dingxi,Gansu,China: 2020.
[18]	张道林. 黑龙江省马铃薯疮痂病病原菌鉴定及Bacillus amyloliquefaciens ZDL38的生防应用[D]. 泰安: 山东农业大学, 2022.doi:10.27277/d.cnki.gsdnu.2022.000882.
	Zhang D L. Identification of potato common scab pathogens in Heilongjiang Province and the biocontrol application of Bacillus amyloliquefaciens ZDL38[D]. Taian: Shandong Agricultural University, 2022.
[19]	姚凡. 宁夏马铃薯疮痂病病原菌分离鉴定及生物防治药剂研究[D]. 银川: 宁夏大学, 2022.doi:10.27257/d.cnki.gnxhc.2022.000090.
	Yao F. Isolation and identification of pathogens of potato scab and screening of biological fungicides for control in Ningxia[D]. Yinchuan: Ningxia University, 2022.
[20]	李宇晨. 内蒙古马铃薯疮痂病菌的分离鉴定及多样性[D]. 呼和浩特: 内蒙古农业大学, 2020.doi:10.27229/d.cnki.gnmnu.2020.000961.
	Li Y C. Isolation,identification and diversity on pathogens of potato common scab in Inner Mongolia[D]. Hohhot: Inner Mongolia University, 2020.
[21]	张翠文. 马铃薯疮痂病菌Streptomyces galilaeus 5T-1果胶酸裂解酶基因CP966_RS08110的功能研究[D]. 兰州: 甘肃农业大学, 2024.doi:10.27025/d.cnki.ggsnu.2024.000355.
	Zhang C W. Functional study of pectate lyases gene CP966_RS08110 in Streptomyces galilaeus 5T-1[D]. Lanzhou: Gansu Agricultural University, 2024.
[22]	郭瑞, 何伟, 罗文芳, 周军辉, 汪雪晶, 许建军, 宋素琴. 甘肃临洮县马铃薯疮痂病致病链霉菌的分离鉴定及致病性[J]. 江苏农业科学, 2024, 52(19):158-164.doi:10.15889/j.issn.1002-1302.2024.19.022.
	Guo R, He W, Luo W F, Zhou J H, Wang X J, Xu J J, Song S Q. Isolation,identification and pathogenicity of pathogenic Streptomyces of potato scab in Lintao County,Gansu Province[J]. Jiangsu Agricultural Sciences, 2024, 52(19):158-164.
[23]	尚书妍, 王金辉, 郭卫卫, 张岱, 潘阳, 赵冬梅, 李倩, 朱杰华, 杨志辉. 防治多种链霉菌引起的马铃薯疮痂病生防芽孢杆菌的筛选[J]. 中国马铃薯, 2023, 37(6):527-535.doi:10.19918/j.cnki.1672-3635.2023.06.005.
	Shang S Y, Wang J H, Guo W W, Zhang D, Pan Y, Zhao D M, Li Q, Zhu J H, Yang Z H. Screening of bio-control Bacillus against potato common scab caused by various Streptomyces[J]. Chinese Potato Journal, 2023, 37(6):527-535.
[24]	Healy F G, Wach M, Krasnoff S B, Gibson D M, Loria R. The txtAB genes of the plant pathogen Streptomyces acidiscabies encode a peptide synthetase required for phytotoxin thaxtomin A production and pathogenicity[J]. Molecular Microbiology, 2000, 38(4):794-804.doi:10.1046/j.1365-2958.2000.02170.x.
[25]	Healy F G, Krasnoff S B, Wach M, Gibson D M, Loria R. Involvement of a cytochrome P450 monooxygenase in thaxtomin a biosynthesis by Streptomyces acidiscabies[J]. Journal of Bacteriology, 2002, 184(7):2019-2029.doi:10.1128/jb.184.7.2019-2029.2002.
[26]	Güldener U, Seong K Y, Boddu J, Cho S, Trail F, Xu J R, Adam G, Mewes H W, Muehlbauer G J, Kistler H C. Development of a Fusarium graminearum Affymetrix GeneChip for profiling fungal gene expression in vitro and in planta[J]. Fungal Genetics and Biology, 2006, 43(5):316-325.doi:10.1016/j.fgb.2006.01.005. pmid: 16531083
[27]	张海颖. 我国北方马铃薯疮痂病菌组成分析与致病菌株分子检测[D]. 保定: 河北农业大学, 2014.
	Zhang H Y. Diversity analysis of potato common scab pathogens in Northern China and molecular detection of pathogenic strains[D]. Baoding: Hebei Agricultural University, 2014.

采集地点 Collection place	不同症状病薯数量/个 Number of diseased potatoes with different symptoms	病斑类型 Type of lesion	分离菌株数量 Number of isolated strains	菌株编号 Strain number
秦皇岛昌黎县Changli County of Qinhuangdao	2,2,3	凸起状、平状、凹陷状	2	CL-1、CL-2
石家庄正定县Zhengding County of Shijiazhuang	6,1	凹陷状、平状	2	SJZ-1、SJZ-2
保定市清苑区Qingyuan District of Baoding	3	凸起状	1	QY
河北农业大学实验农场	2	平状	1	SFC
Experimental farm of Hebei Agricultural University

采集地点 Collection place	不同症状病薯数量/个 Number of diseased potatoes with different symptoms	病斑类型 Type of lesion	分离菌株数量 Number of isolated strains	菌株编号 Strain number
秦皇岛昌黎县Changli County of Qinhuangdao	2,2,3	凸起状、平状、凹陷状	2	CL-1、CL-2
石家庄正定县Zhengding County of Shijiazhuang	6,1	凹陷状、平状	2	SJZ-1、SJZ-2
保定市清苑区Qingyuan District of Baoding	3	凸起状	1	QY
河北农业大学实验农场	2	平状	1	SFC
Experimental farm of Hebei Agricultural University

扩增基因 Amplified gene	引物 Primer	引物序列(5'-3') Primer sequence(5'-3')	片段大小/bp Size of PCR product	退火温度/℃ Annealing temperature
ITS	PA	TTTGATCCTGGCTCAG	1 535	60
	PH	AAGGAGGTGATCCAGCCGCA
textAB	textAB-F	CCACCAGGACCTGCTCTTC	385	53
	textAB-R	TCGAGTGGACCTCACAGATC
necl	necl-F	ATGAGCGCGAACGGAAGCCCCGGA	700	62
	necl-R	GCAGGTCGTCACGAAGGATCG
tomA	tomA-F	GAGGCGTTGGTGGAGTTCTA	392	53
	tomA-R	TTGGGGTTGTACTCCTCGTC

扩增基因 Amplified gene	引物 Primer	引物序列(5'-3') Primer sequence(5'-3')	片段大小/bp Size of PCR product	退火温度/℃ Annealing temperature
ITS	PA	TTTGATCCTGGCTCAG	1 535	60
	PH	AAGGAGGTGATCCAGCCGCA
textAB	textAB-F	CCACCAGGACCTGCTCTTC	385	53
	textAB-R	TCGAGTGGACCTCACAGATC
necl	necl-F	ATGAGCGCGAACGGAAGCCCCGGA	700	62
	necl-R	GCAGGTCGTCACGAAGGATCG
tomA	tomA-F	GAGGCGTTGGTGGAGTTCTA	392	53
	tomA-R	TTGGGGTTGTACTCCTCGTC

菌株编号 Strain number	萝卜苗平均株高/cm Average plant height of radish seedlings	萝卜苗平均根长/cm Average root length of radish seedlings
SJZ-2	8.00±0.612a	9.80±0.570a
CL-2	7.40±0.652a	9.20±0.908ab
SFC	7.20±0.447a	8.90±0.758ab
QY	5.80±0.758b	7.70±0.277b
SJZ-1	5.70±0.570b	5.40±0.652c
CK	7.40±0.418a	9.00±0.707ab

Please choose a citation manager

Content to export