Resistance of Small G Protein StRab5b to Verticillium Wilt of Potato

doi:10.7668/hbnxb.20195675

Abstract

Abstract:

The aim was to explore the role of the small G protein StRab5b in potato resistance to Verticillium wilt.The relative expression levels of the StRab5b gene in the roots of potato tissue culture seedlings(Hutou and Xiapodi)after inoculation with Verticillium dahliae were determined by qRT-PCR.The biomass of V.dahliae in the roots,the lesion area on potato leaves,the content of hydrogen peroxide(H₂O₂),the content of malondialdehyde(MDA),and the activity of antioxidant enzymes were detected in the transgenic potato lines L7,L8,and L10 overexpressing StRab5b(StRab5b-L7,StRab5b-L8,StRab5b-L10)and the non-transgenic plants(CK)after inoculation with V.dahliae.The disease index was calculated using the pot culture method with the StRab5b-L7 transgenic line and the non-transgenic plants(CK).The results showed that the expression of the StRab5b gene was induced in potato after inoculation with V.dahliae.Compared with 0 h after inoculation,the relative expression levels of Hutou and Xiapodi increased significantly by 701.12% and 649.41% at 72 h after inoculation,respectively.After inoculation with V.dahliae,overexpression of StRab5b enhanced the resistance of potato to Verticillium wilt.Compared with CK,the lesion area on potato leaves in StRab5b-L7,StRab5b-L8,and StRab5b-L10 decreased significantly by 78.56%,66.56%,and 59.76% at 96 h after inoculation,respectively;the biomass of V.dahliae in the roots decreased significantly by 73.07%,51.69%,and 23.38% at 168 h after inoculation,respectively;the MDA content decreased significantly by 47.13%,33.08%,and 14.70% at 96 h after inoculation,respectively;the H₂O₂ content increased significantly by 83.67%,48.38%,and 20.15% at 72 h after inoculation,respectively,and the CAT activity increased by 53.54%,33.28%,and 12.62%,respectively;the SOD activity increased significantly by 53.01%,36.36%,and 16.54% at 48 h after inoculation,respectively;the POD activity increased by 55.58%,27.18%,and 1.73% at 96 h after inoculation,respectively;the APX activity increased significantly by 63.69%,44.27%,and 17.11% at 72 h after inoculation,respectively.Compared with CK,the disease rate and disease index of the StRab5b-L7 transgenic line decreased significantly by 43.33 percentage points and 86.07%,respectively.In conclusion,overexpression of the StRab5b gene enhanced the resistance of potato to Verticillium wilt.

Key words: Potato, Small G protein, Verticillium wilt, Disease resistance

CLC Number:

SUN Xuetao, YI Haijiao, SUN Jing, ZHENG Guohua, LIU Zhida, FAN Gen, ZHAO Jun, ZHANG Zhiwei. Resistance of Small G Protein StRab5b to Verticillium Wilt of Potato[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(6): 177-185. doi: 10.7668/hbnxb.20195675.

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Figures/Tables 8

Tab.1 qRT-PCR primer sequence information

引物名称 Primer name	引物序列(5'—3') Primer sequence(5'—3')
q-StActin-F	CACCCTGTTCTGCTCACT
q-StActin-R	CAGCCTGAATAGCAACATAC
Fg-qGFP-F	CGACAAGCAGAAGAACGGCATCAA
Fg-qGFP-R	CTTCTCGTTGGGGTCTTTGCTCA
q-StRab5b-F	ATAGCCTTGCAGGACTCAAC
q-StRab5b-R	CAGCACCTCGGTAGTATAATGG

Fig.1 Relative expression of StRab5b gene after inoculation with Verticillium dahliae Different lowercase letters indicate significant differences(P<0.05).The same as Fig.2—7.

Fig.2 Changes of lesion area on potato leaves after inoculation A.Changes of lesion area on potato leaves after inoculation;B.Quantitative analysis of potato leaves lesion area after inoculation.

Fig.3 Colonization of Verticillium dahliae on potato roots after inoculation A.Fluorescence intensity in potato roots after inoculation;B.Quantitative analysis of average fluorescence intensity of potato root after inoculation;C.The biomass of Verticillium dahliae colonizing potato roots after inoculation.

Fig.4 Changes in MDA content in potato leaves after inoculation

Fig.5 Changes in hydrogen peroxide content in potato leaves after inoculation A.DAB staining of potato leaves after inoculation;B.Changes in hydrogen peroxide content in potato leaves after inoculation.

Fig.6 Activities of CAT,SOD,POD and APX in potato leaves after inoculation

Fig.7 Incidence of potted plants inoculated with Verticillium dahlia A.Phenotypic symptoms of the strain.

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