Identification of Genes Associated with Body Weight at 8 Weeks Age in Kangle Yellow Chickens Based on Omics Data

doi:10.7668/hbnxb.20195294

Abstract

Abstract:

The purpose of this study is to screen candidate genes related to the body weight at 8 weeks age of Kangle yellow chicken based on the omics data,and provide the theoretical foundation for molecular marker-assisted breeding of growth traits in Kangle yellow chickens.It also provides key basic data for improving molecular breeding methods for high-quality broilers and accelerating,the progress of breed selection.To detect SNPs significantly associated with body weight traits at 8 weeks age of Kangle yellow chickens,the body weight was measured from 8 to 22 weeks of age of 434 Kangle yellow chickens.Genome-wide association study(GWAS)was performed using the gene chip technology.Genes in the candidate regions with 2 Mb windows surrounding each significant SNP were found for GO and KEGG function analysis.Combined with the data of transcriptomic sequencing and published literature,key candidate genes related to the body weight at 8 weeks age of Kangle yellow chicken were screened.Two potential SNPs significantly associated with target traits were detected,located on chromosome 2(131 485 613 bp)and chromosome 4(60 413 848 bp).A total of 118 candidate genes were screened near SNP sites.Gene function annotation analysis showed the most significant enrichment of biological processes was retinoic acid metabolism.The significant enrichment of 12 KEGG pathways was found,including fatty acid degradation,tyrosine metabolism and drug metabolism-cytochrome P450.OXR1, RSPO2,EIF3E,TRHR,BMPR1B, ADH1C, MTTP, LAMTOR3, PPP3CA and PDLIM3 were preliminarily identified as key candidate genes for body weight traits at 8 weeks age of Kangle yellow chickens.Two SNPs and 10 key candidate genes were preliminarily identified related to the body weight at 8 weeks age of Kangle yellow chicken.

Key words: Kangle yellow chicken, SNP, GWAS, Body weight, Candidate gene, Gene pathway

MA Jinge, CHEN Yujie, LEI Wenjing, XU Qiao, XU Jiguo, XU Jing, RAO Yousheng. Identification of Genes Associated with Body Weight at 8 Weeks Age in Kangle Yellow Chickens Based on Omics Data[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(1): 224-231. doi: 10.7668/hbnxb.20195294.

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Figures/Tables 10

References 31

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性状 Trait	统计量/只 Statistical magnitude	最大值/g Maximum value	最小值/g Minimum value	平均值/g Mean	标准差 Standard deviation	变异系数/% Coefficient of variation	相关系数 Correlation coefficient
8周龄体质量 Weight at 8 weeks	414	650	187	479.23	51.40	10.73	1.000
10周龄体质量 Weight at 10 weeks	414	800	400	582.13	63.93	10.98	0.835^**
12周龄体质量 Weight at 12 weeks	413	950	500	703.87	78.65	11.17	0.265^**
14周龄体质量 Weight at 14 weeks	406	1 200	600	818.23	98.70	12.06	0.291^**
16周龄体质量 Weight at 16 weeks	404	1 500	650	790.17	138.34	14.26	0.233^**
22周龄体质量 Weight at 22 weeks	413	1 800	900	1 282.20	174.96	13.64	0.124^*

性状 Trait	统计量/只 Statistical magnitude	最大值/g Maximum value	最小值/g Minimum value	平均值/g Mean	标准差 Standard deviation	变异系数/% Coefficient of variation	相关系数 Correlation coefficient
8周龄体质量 Weight at 8 weeks	414	650	187	479.23	51.40	10.73	1.000
10周龄体质量 Weight at 10 weeks	414	800	400	582.13	63.93	10.98	0.835^**
12周龄体质量 Weight at 12 weeks	413	950	500	703.87	78.65	11.17	0.265^**
14周龄体质量 Weight at 14 weeks	406	1 200	600	818.23	98.70	12.06	0.291^**
16周龄体质量 Weight at 16 weeks	404	1 500	650	790.17	138.34	14.26	0.233^**
22周龄体质量 Weight at 22 weeks	413	1 800	900	1 282.20	174.96	13.64	0.124^*

染色体 Chromosome	物理位置/bp Position	P值 P value	基因型 (个体数) Genotype (Number)	基因型频率 Genotype frequency	体质量/g Body weight
2	131 485 613	8.48×10^-6	CC (26)	0.16	429.04±67.12a
			CG (155)	0.38	476.67±53.08b
			GG (207)	0.51	487.41±44.74b
4	60 413 848	1.12×10^-5	CC (164)	0.40	465.90±49.79a
			TC (199)	0.49	485.08±49.79b
			TT (45)	0.11	501.04±52.89c

染色体 Chromosome	物理位置/bp Position	P值 P value	基因型 (个体数) Genotype (Number)	基因型频率 Genotype frequency	体质量/g Body weight
2	131 485 613	8.48×10^-6	CC (26)	0.16	429.04±67.12a
			CG (155)	0.38	476.67±53.08b
			GG (207)	0.51	487.41±44.74b
4	60 413 848	1.12×10^-5	CC (164)	0.40	465.90±49.79a
			TC (199)	0.49	485.08±49.79b
			TT (45)	0.11	501.04±52.89c

引物名称 Primer name	SNP位置/bp SNP position	引物序列(5'—3') Primer sequence(5'—3')	退火温度/℃ Annealing temperature	片段长度/bp Fragment length
1	Chr2:131 485 613	F:ACATGACGGCAAGGTAACGTA	60	268
		R:CCAGAGCACACACACTCCTC
2	Chr4:60 413 848	F:AGCCAATGTCCAAGCATACCA	60	314
		R:GGTGGCATTGCAGCACTATTC

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