Comparation and Analysis of Milk Composition,Serum Biochemistry and Lactation-related Genes of Bamei Pigs and Yorkshire Pigs

doi:10.7668/hbnxb.20195277

Abstract

Abstract:

This study aimed to compare and analyze the milk composition,serum biochemical indices and expression levels of Leptin and LF genes during lactation between Bamei pigs and Yorkshire pigs.The six purebred Bamei pigs and Yorkshire pigs with healthy body conditions were selected respectively,which also had close parity,mating and delivery periods(within three days).A total of 50 mL milk,2 mL serum and 2 mL whole blood samples were collected from Bamei pigs and Yorkshire pigs on the 1st,7th,14th,21st and 28th day after delivery,respectively.Then,the milk,serum and whole blood samples were used to detect the milk compositions,serum indexes of alkaline phosphatase(ALP),albumin(ALB),globulin(GLB),triglyceride(TG),urea nitrogen(BUN),alanine aminotransferase(ALT)and aspartate aminotransferase(AST),and the expression levels of Leptin and LF genes.The results showed that on the 1st day of lactation in Baimei pigs,in addition to milk fat(Fat),the content of casein(CS),total protein(TP),total solid matter(TS),non-fat milk solids(SNF),lactose(L),free fatty acid(FFA)and acidity in the colostrum of Bamei pigs were all extremely significantly higher than those in the colostrum of Yorkshire pigs,while,the content of CS,TP,Fat,TS,SNF and L of Bamei pigs on the 21st day after delivery were extremely significantly lower than those of Yorkshire pigs.The serum biochemical indexes of two pig breeds showed different trends during the whole lactation.The content of ALT,AST,GLB,BUN and TG of Bamei pigs were significantly higher than those of Yorkshire pigs on the 1st day after delivery,while the content of ALP and ALB were all significantly lower than those of Yorkshire pigs.There were no different levels of these two genes on the 1st day,however,from the 7th day after delivery,the expression levels of LF in the Bamei pigs were significantly different than those in the Yorkshire pigs,while Leptin gene was extremely significantly expressed in Yorkshire pigs than in Bamei pigs.In general,the milk nutrients of Bamei and Yorkshire pigs showed a decreasing trend as the extension of the lactation periods,and the expression levels of Leptin and LF genes were likely related to the milk yield and quality between the two breeds,which may need to be further verified.

Key words: Bamei pig, Yorkshire pig, Lactation period, Milk composition, Serum biochemical index, Gene expression

FU Panpan, HU Huihui, LI Jie, ZHAO Jin, WANG Pengfei, SHANG Xuefeng, ZHANG Sen, HUANG Xiaoyu. Comparation and Analysis of Milk Composition,Serum Biochemistry and Lactation-related Genes of Bamei Pigs and Yorkshire Pigs[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(1): 215-223. doi: 10.7668/hbnxb.20195277.

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References 36

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基因 Gene	引物序列(5'—3') Primer sequence(5'—3')	退火温度/℃ Annealing temperature	产物长度/bp Product length
Leptin	F:TTGCAGAGCTCTTCCTCACG	59.5	184
	R:TCAACGGGCATTCAACCGTA
LF	F:GTCACAGCCATCGCTAACCT	59.5	109
	R:TTGCTCCTCCAAGCTTGACC
GAPDH	F:AGCAATGCCTCCTGTACCAC	59.5	210
	R:AAGCAGGGATGATGTTCTGG

基因 Gene	引物序列(5'—3') Primer sequence(5'—3')	退火温度/℃ Annealing temperature	产物长度/bp Product length
Leptin	F:TTGCAGAGCTCTTCCTCACG	59.5	184
	R:TCAACGGGCATTCAACCGTA
LF	F:GTCACAGCCATCGCTAACCT	59.5	109
	R:TTGCTCCTCCAAGCTTGACC
GAPDH	F:AGCAATGCCTCCTGTACCAC	59.5	210
	R:AAGCAGGGATGATGTTCTGG

营养成分 Nutrient	泌乳时期 Lactation periods
营养成分 Nutrient	1 d	7 d	14 d	21 d	28 d
酪蛋白/% CS	16.56±0.26Aa	4.29±0.08Bb	4.19±0.04Bb	2.98±0.06Cc	4.23±0.15Bb
总蛋白/% TP	23.87±0.31Aa	4.78±0.15Bb	4.93±0.11Bb	4.19±0.08Cc	4.92±0.11Bb
乳脂/% Fat	6.32±0.07Cc	8.33±0.21Aa	7.70±0.10Bb	5.26±0.11Dd	6.37±0.08Cc
总固形物/% TS	36.47±0.04Aa	20.61±0.08Bb	19.66±0.20Cc	14.66±0.71Ee	18.75±0.10Dd
非脂乳固体/% SNF	28.17±0.25Aa	11.17±0.06Bb	11.14±0.07Bb	8.45±0.07Cc	11.37±0.08Bb
乳糖/% L	13.71±0.20Aa	5.63±0.10Aab	5.60±0.07Aab	5.50±0.21Ab	5.64±0.07Aab
酸度/°T Acidity	82.68±0.19Aa	8.48±0.10Bb	8.45±0.08Bb	6.81±0.07Cc	8.66±0.09Bb
游离脂肪酸/% FFA	84.74±0.22Aa	26.43±0.27Bb	26.30±0.40Bb	16.90±0.31Dd	25.40±0.30Cc

营养成分 Nutrient	泌乳时期 Lactation periods
营养成分 Nutrient	1 d	7 d	14 d	21 d	28 d
酪蛋白/% CS	16.56±0.26Aa	4.29±0.08Bb	4.19±0.04Bb	2.98±0.06Cc	4.23±0.15Bb
总蛋白/% TP	23.87±0.31Aa	4.78±0.15Bb	4.93±0.11Bb	4.19±0.08Cc	4.92±0.11Bb
乳脂/% Fat	6.32±0.07Cc	8.33±0.21Aa	7.70±0.10Bb	5.26±0.11Dd	6.37±0.08Cc
总固形物/% TS	36.47±0.04Aa	20.61±0.08Bb	19.66±0.20Cc	14.66±0.71Ee	18.75±0.10Dd
非脂乳固体/% SNF	28.17±0.25Aa	11.17±0.06Bb	11.14±0.07Bb	8.45±0.07Cc	11.37±0.08Bb
乳糖/% L	13.71±0.20Aa	5.63±0.10Aab	5.60±0.07Aab	5.50±0.21Ab	5.64±0.07Aab
酸度/°T Acidity	82.68±0.19Aa	8.48±0.10Bb	8.45±0.08Bb	6.81±0.07Cc	8.66±0.09Bb
游离脂肪酸/% FFA	84.74±0.22Aa	26.43±0.27Bb	26.30±0.40Bb	16.90±0.31Dd	25.40±0.30Cc

营养成分 Nutrient	泌乳时期 Lactation periods
营养成分 Nutrient	1 d	7 d	14 d	21 d	28 d
酪蛋白/% CS	11.76±0.04Aa	4.22±0.12Cc	3.87±0.09Dd	4.07±0.06CDcd	4.96±0.19Bb
总蛋白/% TP	16.77±0.04Aa	6.12±0.09Cc	4.54±0.06De	4.73±0.15Dd	6.09±0.04Bb
乳脂/% Fat	6.36±0.05Cc	7.40±0.24Bb	7.60±0.19Bb	7.66±0.06Bb	8.17±0.06Aa
总固形物/% TS	28.81±0.05Aa	19.43±0.09Cc	19.09±0.07Dd	19.34±0.13CDc	21.17±0.12Bb
非脂乳固体/% SNF	21.05±0.06Aa	11.25±0.11Bb	10.87±0.06Bb	10.93±0.18Bb	15.11±5.24ABb
乳糖/% L	2.53±0.05Dd	5.35±0.11Bb	5.70±0.04Aa	5.74±0.17Aa	5.08±0.05Cc
酸度/°T Acidity	57.17±0.06Aa	9.62±0.13Cc	7.10±0.04Ee	7.56±0.23Dd	10.63±0.25Bb
游离脂肪酸/% FFA	66.28±0.13Aa	30.75±0.70Bb	24.52±0.27Cc	25.00±3.61Cc	22.40±0.30Cc

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