Effect of Top Application of Nitrogen Fertilizer on the Activities of Nitrogen Metabolizing Enzymes in Leaves of Different Forage Oats in Sandy Land

doi:10.7668/hbnxb.20191727

Abstract

Abstract: In order to study the physiological response and difference of nitrogen metabolizing enzyme activity in leaves of different forage oats in Horqin Sandy Land. The forage oats of Muwang and Tianyan No.1 were applied with 0 (CK), 100, 200, 300 kg/ha pure nitrogen in the proportion of 15%, 40%, 25% and 20% at tillering, jointing, heading and flowering stages. The activities of glutamate synthase (GOGAT), glutamine synthetase (GS), nitrate reductase (NR), glutamic oxaloacetic transaminase (GOT), glutamic pyruvate transaminase (GPT) were measured in flag leaf, inverted two leaf and converse third leaf during the filling period, and the differences of nitrogen metabolizing enzyme activities and their relationship with hay yield of different forage oat varieties under nitrogen application rate were analyzed. The results showed that the hay yield of Muwang and Tianyan No.1 reached the maximum at 200,300 kg/ha nitrogen levels, respectively;The activities of GOGAT, GS, NR, GOT and GPT in the leaves of Muwang and Tianyan No.1 forage oats increased first and then decreased with the increase of nitrogen application, and the enzyme activity was the strongest under N₂₀₀ nitrogen application (except for GOT activity of converse third leaf under N₃₀₀ treatment).Except for GS activity of the third leaf of N₁₀₀ treatment and GPT activity of the second leaf in different nitrogen treatments, GOGAT, NR, GS, GOT and GPT enzyme activities of Muwang were higher than those of Tianyan No.1. This indicated that nitrogen assimilation ability of forage oat variety Muwang was stronger than that of Tianyan No.1.Therefore, in Horqin Sandy Land, the suitable amount of topdressing nitrogen fertilizer for planting Muwang was 200 kg/ha, while for planting Tianyan No.1, topdressing nitrogen fertilizer of 300 kg/ha could obtain higher hay yield. GOT and GPT activities were the key enzymes for screening forage oat varieties with high nitrogen use efficiency.

Key words: Feed oats, Nitrogen application amount, Nitrogen metabolizing enzymes, Physiological response

CLC Number:

S143.1

ZHANG Yuxia, WANG Xin, ZHANG Qingxin, Siriguleng, GUO Yuan, ZHU Aimin. Effect of Top Application of Nitrogen Fertilizer on the Activities of Nitrogen Metabolizing Enzymes in Leaves of Different Forage Oats in Sandy Land[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 159-165. doi: 10.7668/hbnxb.20191727.

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