E2 Regulates the Expression of COX-2 in Endometrial Epithelial Cells of Cows Through p38 MAPK and PI3K/Akt Signaling Pathway

doi:10.7668/hbnxb.20191025

Abstract

Abstract: To investigate whether p38 MAPK and PI3K/Akt signaling pathways are involved in the regulation of estradiol(E₂) on PGF_2α secretion in cow endometrial epithelial cells, endometrial epithelial cells of pregnant dairy cows were cultured in vitro, and different concentrations of E₂ and p38 MAPK and PI3K/Akt pathway inhibitors SB 203580 and LY294002 were used to treat cows' endometrial epithelial cells respectively. The expression of COX-2 gene and protein and the level of PGF_2α were detected by Western Blot, qRT-PCR and ELISA. The results showed that E₂ promoted the expression of COX-2 and the secretion of PGF_2α. In addition, E₂ activated the p38 MAPK and PI3K/Akt signaling pathways. Moreover, the addition of SB203580 and LY294002 inhibit the promotion effect of E₂ on COX-2 expression and PGF_2α secretion. Therefore, it proved that E₂ promoted the expression of COX-2 and the secretion of PGF_2α by activating the p38 MAPK and PI3K/Akt signaling pathways.

Key words: Cow, Endometrial epithelial cells, Estradiol, Cyclooxygenase-2, Prostaglandin F_2α

CLC Number:

S795.7

FENG Ziyan, WANG Xiangguo, YUAN Mengyi, FU Bofan, XIE Tongtong, CHANG Di, WU Chunyang, NI Hemin, XIAO Longfei. E₂ Regulates the Expression of COX-2 in Endometrial Epithelial Cells of Cows Through p38 MAPK and PI3K/Akt Signaling Pathway[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(1): 232-238. doi: 10.7668/hbnxb.20191025.

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E₂ Regulates the Expression of COX-2 in Endometrial Epithelial Cells of Cows Through p38 MAPK and PI3K/Akt Signaling Pathway

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