Studies on the Expression of Phosphodiacylglycerol Acyltransferase (BnPDAT1) in Brassica napus

doi:10.7668/hbnxb.201751114

Abstract

Abstract: This paper aims to investigate the relationship between the expression characteristics of BnaPDAT1 gene and oil synthesis in Brassica napus L.. Two Brassica napus L. varieties, double-low strains 855 (49.72%) and 868 (35.06%), were selected as materials, from which 3 copies of BnaPDAT1 were detected by qRT-PCR. The regularity was expressed, and the accumulation of TAG in the two strains of rapeseed was detected by thin layer chromatography (TLC) and gas chromatography (GC). The results showed that the expression of BnaPDAT1 gene and its three copies in seeds increased first and then decreased after pollination. BnaPDAT1 was expressed in flowers and leaves, but the expression of three copies was different, but showed the characteristics of overall regulation. The expression levels of BnaPDAT1 and its three copies in leaves (except BnaA10.PDAT1) and seeds 20 days after pollination were significantly different between the two strains. The expression patterns of BnaPDAT1 and its three copies were not obvious between the two strains at the other stages. The expression level of BnaPDAT1 in the high oil line 855 reached to the highest value at 20 days after pollination (11.100 9), which was 5.07 times that of line 868, and the expression level in leaves was 8.858 6, 7.34 times that of 868. The expression characteristics was similar to that of BnaC09.PDAT1. The TAG content of rapeseed seeds in both strains presented a S-type. The oil synthesis entered a rapid growth stage after 20 days of pollination, and entered a slow growth period after 35 days of pollination. The TAG content had no significant difference between the two lines at 30 days after pollination, and showed some differences after 35 days of pollination. After 40 days, the difference further expanded and stabilized. There was no significant direct relationship between BnaPDAT1 gene expression and seed TAG content, but the expression level of BnaPDAT1 gene in high oil line was significantly higher than that in low oil line.

Key words: Brassica napus, Phosphatidylglycerol acyltransferase (BnaPDAT1), Gene, Expression characteristics

CLC Number:

Q78
S511.03

TAN Tailong, FENG Tao, LUO Haiyan, PENG Ye, LIU Ruiyang, GUAN Chunyun. Studies on the Expression of Phosphodiacylglycerol Acyltransferase (BnPDAT1) in Brassica napus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 12-18. doi: 10.7668/hbnxb.201751114.

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