Transcriptional Analysis of Rice Root under Nitrogen Deficiency

doi:10.7668/hbnxb.2018.01.025

Abstract

Abstract: Rice development should be effected by nutrient deficiency,and the root was respondent to environmental stress earlier than shoot.To reveal the mechanism of nitrogen stress on rice root,the transcriptome of rice root was analyzed under nitrogen stress.The Illumina Hiseq2000 high-throughput sequencing technology was used to get the comprehensive transcriptome from rice root in response to nitrogen stress,and a total of 2 270 differentially expressed Unigenes were generated including 1 176 up-regulated Unigenes and 1 094 down-regulated Unigenes.With GO functional classifications and Pathways,all differentially expression Unigenes were grouped into 48 categories and 118 metabolisms,respectively,in which many functional metabolisms,such as glycolysis,fatty acid metabolism,oxidative phosphorylation,amino acid metabolism,starch and sucrose metabolism,and so on,were included.The function of some differentially expression Unigenes in root were analyzed,and the results indicated that a lot of new proteins were induced for enzyme by nitrogen stress,which involved in the new RNA and protein synthesis.The Unigenes associated with polar auxin transport to root,plant cell growth and enzymic synthesis of lignin precursors were up-regulated greatly,which resulted in longer root.

Key words: Rice, Root, Nitrogen stress, Transcriptome

CLC Number:

SHAO Caihong, LI Yao, QIAN Yinfei, CHEN Jin, CHEN Xianmao, GUAN Xianjiao, LIU Guangrong, PENG Chunrui, QIU Caifei. Transcriptional Analysis of Rice Root under Nitrogen Deficiency[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(1): 168-175. doi: 10.7668/hbnxb.2018.01.025.

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