Development and Application of Two Functional Markers for Grain Chalkiness Gene Chalk5 in Rice

doi:10.7668/hbnxb.2017.01.001

Abstract

Abstract: Grain chalkiness is one of the important indicators to evaluate the quality of rice appearance. Reducing grain chalkiness is the basis for high quality rice breeding. Chalk5 is a major quantitative trait locus which has been cloned and can influence grain chalkiness. In order to increase the selection efficiency of Chalk5 in breeding, six pairs of amplification refractory mutation system PCR (ARMS-PCR) primers had been designed basing on two functional mutations in promoter region. Finally, two pairs of functional markers,Chalk12/NChalk12 and Chalk22/NChalk22 had been selected out. The sequenced results of target region were consisted with the PCR results which verified the detection accuracy of the newly designed markers. Chalk12/NChalk12 and Chalk22/NChalk22 were employed to genotype 43 indica rice varieties, 65 japonica varieties certificated in Jiangsu from 2007 to 2013, and 179 local japonica cultivars from Taihu Basin. The results showed that only 20 indica rice varieties and 8 local japonica cultivars from Taihu Basin carried chalk5 gene, while the other 259 rice varieties carried Chalk5 gene. All these indicated that chalk5 mainly existing in indica germplasm.

Key words: Rice, Chalkiness, Chalk5, Amplification refractory mutation system PCR, Functional marker

CLC Number:

ZHU Jinyan, WANG Qingqing, WANG Jun, FAN Fangjun, LI Wenqi, WANG Fangquan, WANG Xupeng, ZHONG Weigong, YANG Jie. Development and Application of Two Functional Markers for Grain Chalkiness Gene Chalk5 in Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(1): 1-8. doi: 10.7668/hbnxb.2017.01.001.

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