Molecular Cloning and Expression Analysis of Flowering-regulating Transcription Factor PmSOC1-like Gene in Prunus mume

doi:10.7668/hbnxb.2016.05.012

Abstract

Abstract: The MADS-box gene SOC1/TM3 (Suppressor of overexpression of constans 1/Tomato MADS-box gene 3) integrates multiple flowering signals to regulate the transition from vegetative to reproductive development in plants . To better understand the molecular regulation of flower development in Prunus mume,three putative orthologs of SOC1,including PmSOC1-1, PmSOC1-2, and PmSOC1-3,were isolated.The expression patterns of PmSOC1-1, PmSOC1-2 and PmSOC1-3 were determined by Real-time RT-PCR.Each PmSOC1-like gene has a single long open reading frame(ORF) encoding a putative protein of 214,217 and 219 amino acids respectively.Phylogenetic analysis suggested that PmSOC1-1, PmSOC1-2,and PmSOC1-3 genes might be orthologous of Arabidopsis SOC1, AGL42/71/72,and AGL14/19,respectively.By Real-time quantitative RT-PCR experiment,three PmSOC1-like genes were mainly expressed in vegetative organs and expressed at low level in reproductive organs such as flowers,fruit,seed and so on.The expression of the three PmSOC1-like genes showed a decreasing trend.Above results suggest that the PmSOC1-like gene plays an important role in the transition from the vegetative growth to the reproductive growth phase.

Key words: Prunus mume, PmSOC1-like, Gene cloning, Expression analysis

CLC Number:

S685.17

LI Yushu, YANG Weiru, CHENG Tangren, WANG Jia, ZHANG Qixiang. Molecular Cloning and Expression Analysis of Flowering-regulating Transcription Factor PmSOC1-like Gene in Prunus mume[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(5): 78-85. doi: 10.7668/hbnxb.2016.05.012.

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