Preliminary Study on Biological Effects of Maize Seeds Irradiated Using 252Cf Fission Neutron Source

doi:10.7668/hbnxb.2016.05.011

Abstract

Abstract: To study the biological effects and rules of neutron irradiation on maize seeds.Maize inbred lines LY8405 and PH6WC were irradiated by using²⁵²Cf fission neutron source,screening suitable neutron irradiation dose and creating mutants for gene mapping,cloning and its functional analysis.The results showed that the germination rate of LY8405 was descended after neutron irradiation at low absorbed doses(0.36-4.19 Gy),however that of PH6WC was increased.With irradiation dose increasing,the rates of distortion,mutation and total genetic variation of the two materials M₁ were all added.LY8405 showed the most sensitively biological effects at 4.19 Gy irradiation dose,the rates of distortion,mutation and total genetic variation were 27.8%,38.9% and 66.7%,respectively;PH6WC showed the most sensitive effect at 2.48 Gy irradiation dose,the rates of distortion,mutation and total genetic variation were 7.1%,14.3% and 21.4%,respectively.Besides,the coefficient of variation of the agronomic characters,including plant height,ear position,leaf number,tassel branch number,spike length,ear diameter,kernel rows per spike and axis width,were all raised with the rising of irradiation dose.The paper indicated that the sensitive degree of maize genotype was different to fast neutron radiation dose;initially considered that 2.00-4.19 Gy was suitable dose of irradiate maize seed using²⁵²Cf fission neutron source.

Key words: Maize, ²⁵²Cf, Neutron, Biology effects, Irradiating doses

CLC Number:

S513.03

LIU Zhongxiang, XU Dapeng, YAO Zeen, KOU Sirong, WANG Xiaojuan, YANG Yanzhong, LIAN Xiaorong, ZHOU Yuqian, HE Haijun, LI Zhiming. Preliminary Study on Biological Effects of Maize Seeds Irradiated Using ²⁵²Cf Fission Neutron Source[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(5): 71-77. doi: 10.7668/hbnxb.2016.05.011.

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Preliminary Study on Biological Effects of Maize Seeds Irradiated Using ²⁵²Cf Fission Neutron Source

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