Effect of Overexpression of cdc20 during Cashmere Goat Oocyte Maturation in vitro

doi:10.7668/hbnxb.2015.06.005

Abstract

Abstract: In order to investigate the effect of overexpression of cdc20 during cashmere goat oocyte maturation in vitro ,gMyc- cdc20 vector was constructed and overexpressed in the oocyte by microinjection after transcripted into cRNA in vitro.The results showed that the normal oocytes needed 8 h from GV stage to GVBD stage,and 18 h to MⅠ.Twenty-four hours later,75% of these oocytes extruded first polar body.Then microinjected gMyc- cdc20 cRNA into GV stage oocytes and cultured them in vitro.MYC-CDC20 fusion protein could be detected by Western Blotting after 10 h.However,both of the development time and the ratio of oocyte maturation were not significantly different compared with those of control.It could conclude that overexpression of cdc20 alone had no effect on the oocyte maturation of cashmere goat in vitro.

Key words: Overexpression, cdc20, Cashmere goat oocyte, Maturation in vitro

CLC Number:

S826

ZHANG Jin-dun, WANG Biao, LI Yun-xia, CHEN Yang-lin, LIU Li-ming, DAI Yan-feng, LI Xi-he. Effect of Overexpression of cdc20 during Cashmere Goat Oocyte Maturation in vitro[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(6): 32-36. doi: 10.7668/hbnxb.2015.06.005.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2015.06.005

http://www.hbnxb.net/EN/Y2015/V30/I6/32

References

[1] Hartwell L H,Weinert T A.Checkpoints:controls that ensure the order of cell cycle events[J].Science,1989,246(4930):629-634.
[2] Kulukian A,Han J S,Cleveland D W.Unattached kinetochores catalyze production of an anaphase inhibitor that requires a Mad2 template to prime Cdc20 for BubR1 binding[J].Developmental Cell,2009,16(1):105-117.
[3] Musacchio A,Salmon E D.The spindle-assembly checkpoint in space and time[J].Nature Reviews Molecular Cell Biology Journal,2007,8(5):379-393.
[4] Yu H.Structural activation of Mad2 in the mitotic spindle checkpoint:the two-state Mad2 model versus the Mad2 template model[J].Journal of Cell Biology,2006,173(2):153-157.
[5] Santaguida S,Musacchio A.The life and miracles of kinetochores[J].EMBO J,2009,28(17):2511-2531.
[6] Amon A.The spindle checkpoint[J].Current Opinion in Genetics & Development,1999,9(1):69-75.
[7] Iwanaqa Y,Kasai T,Kibler K,et al .Characterization of regions in hsMAD1 needed for binding hsMAD2:A polymorphic change in an hsMAD1 leucine zipper affects MAD1-MAD2 interaction and spindle checkpoint function[J].Journal of Biological Chemistry,2002,277(34):31005-31013.
[8] Li Y,Benezra R.Identification of a human mitotic checkpoint gene:hsMAD2 [J].Science,1996,274(5285):246-248.
[9] Waizenegger I,Gimenez-Abian J F,Wernic D,et al .Regulation of human separase by securin binding and autocleavage[J].Current Biology,2002,12(16):1368-1378.
[10] Hauf S,Waizenegger I C,Peters J M.Cohesin cleavage by separase required for anaphase and cytokinesis in human cells[J].Science,2001,293(5333):1320-1323.
[11] Nasmyth K,Haering C H.The structure and function of SMC and kleisin complexes[J].Annual Review of Biochemistry,2005,74:595-648.
[12] Peters J M,Tedeschi A,Schmitz J.The cohesin complex and its roles in chromosome biology[J].Genes & Development,2008,22(22):3089-3114.
[13] Jin F,Hamada M,Malureanu L,et al .Cdc20 is critical for meiosisⅠand fertility of female mice[J].PLOS Genetics,2010,6(9):e1001147.
[14] Tsurumi C,Hoffmann S,Geley S,et al .The spindle assembly checkpoint is not essential for CSF arrest of mouse oocytes[J].Journal of Cell Biology,2004,167(6):1037-1050.
[15] Holt J E,Lane S I,Jones K T.The control of meiotic maturation in mammalian oocytes[J].Current Topics in Developmental Biology,2013,102:207-26.
[16] 杨文琳,安鹏,李伟,等.超表达Cdc20 基因不影响牛卵母细胞第一极体排出[J].中国生物化学与分子生物学报,2012,28(6):546-554.
[17] Karra H,Repo H,Ahonen I,et al .Cdc20 and securin overexpression predict short-term breast cancer survival[J].British Journal of Cancer,2014,110(12):2905-2913.

Please choose a citation manager

Content to export