The Genetic Diversity of 56 Maize Inbred Lines on MultiNA Microchip Electrophoresis Technology

doi:10.7668/hbnxb.2015.06.016

Abstract

Abstract: In order to study the genetic diversity of 56 maize inbred lines,fully tap the genetic potential of the germplasm,the genetic diversity of 56 maize inbred lines were analyzed on MultiNA Microchip Electrophoresis Technology by 36 pairs of Polymorphic SSR markers and their genetic diversity and population structure was revealed by PowerMarker V3.25 and Structure V2.3.4 software.The results showed that:The detected alleles of 36 SSR primers ranged from 8 to 39,with an average of 24.694;genetic diversity ranged from 0.758 to 0.968,with an average of 0.918;the polymorphism information index(PIC)ranged from 0.722 to 0.967,with an average of 0.911.These inbred lines could be divided into seven groups,followed by Lüdahonggu group,Lancaster group,Reid group,P group,Tangsipingtou group,Integrated species subgroup and Tropical germplasm.Because the cluster analysis showed that when K was seven the ΔK value was the maximum.These results provided basis for the test material parental material selection.

Key words: SSR, Maize, Inbred lines, High throughput, Genetic diversity

CLC Number:

S513.03

CAI Miao-miao, SONG Xi-yun, CAI Chun-mei, PEI Yu-he, ZHAO Mei-ai. The Genetic Diversity of 56 Maize Inbred Lines on MultiNA Microchip Electrophoresis Technology[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(6): 105-111. doi: 10.7668/hbnxb.2015.06.016.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2015.06.016

http://www.hbnxb.net/EN/Y2015/V30/I6/105

References

[1] 李伟忠.玉米自交系遗传多样性与表型性状关联分析及分子ID构建[D].哈尔滨:东北农业大学,2011.
[2] 石云素.玉米重要自交系遗传多样性分析及产量相关性状QTL[D].北京:中国农业科学院,2008.
[3] 刘俊,尹晓红,郭志富,等.70份玉米自交系的SSR遗传多样性及杂种优势群分析[J].湖南农业科学,2014,53(6):1256-1258.
[4] 桑志勤,陈树宾,段震宇,等.新疆中熟玉米自交系配合力分析和杂种优势群研究[J].西北农业学报,2014,23(3):31-35.
[5] 李锐,白建荣,张丛卓,等.山西中晚熟玉米区玉米自交系的遗传多样性及杂种优势群分析[J].山西农业科学,2013,41(4):311-316.
[6] 王巍,王远路.20份玉米农家种质优势类群的划分和优势模式分析[J].玉米科学,2013,21(4):11-14.
[7] 骈越斌,许晶,武岩军,等.SSR分子标记技术在玉米杂种优势群划分中的应用[J].山西农业科学,2012,40(5):441-444.
[8] 吕学高,赵军华,楼肖成,等.30份特异玉米自交系的SSR聚类分析[J].浙江农业学报,2014,26(2):274-278.
[9] 王凤格,田红丽,赵久然,等.中国328个玉米品种(组合)SSR标记遗传多样性分析[J].中国农业科学,2014,47(5):856-864.
[10] 王安贵,陈泽辉,李娟,等.北美温带玉米杂交种选系的遗传多样性研究[J].玉米科学,2014,22(1):7-14.
[11] 王兵伟,覃嘉明,黄安霞,等.SSR分子标记分析60份玉米自交系的遗传多样性[J].西南农业学报,2014,27(4):1358-1362.
[12] 孙峰成,冯勇,赵瑞霞,等.12个玉米群体的SSR遗传多样性分析[J].华北农学报,2013,28(5):90-95.
[13] 黄君,冯发强,王青峰,等.56份甜玉米自交系的SSR遗传多样性分析[J].华南农业大学学报,2012,33(1):1-4.
[14] 刘志斋,吴讯,刘海利,等.基于40个核心SSR标记的820份中国玉米重要自交系的遗传多样性与群体结构[J].中国农业科学,2012,45(1):2107-2138.
[15] 胡承香,张玉纯,李磊,等.SDS-聚丙烯酰胺凝胶电泳技术的改良及其应用[J].第三军医大学学报,1996,18(1):68-70.
[16] 张莎莎,郭新梅,赵美爱,等.微芯片电泳与聚丙烯酰胺凝胶电泳的比较分析[J].中国农业科技导报,2013,15(2):173-178.
[17] 郭尧君.SDS电泳技术的实验考虑及最新进展[J].生物化学与生物物理进展,1991,18(1):32-37.
[18] 石晶,张德兴,贺新红,等.丙烯酰胺神经毒性的研究进展[J].解剖学研究,2009,31(4):287-290.
[19] 卢振宇,李明顺,谢传晓,等.玉米叶片DNA快速提取方法改进研究[J].玉米科学,2008,16(2):50-53,55.
[20] Hussain A,Lukow O M.Characterization of the 1B/1R translocation in wheat using water extractable protein concentrate[J].Euphytica,1994,78:109-113.
[21] Liu J,Muse S.PowerMarker:An integrated analysis environment for genetic marker analysis[J].Bioinformatics,2005,21:2128-2129.
[22] Pritchard J K,Stephens M,Donnelly P.Inference of population structure using multilocus genotype data[J].Genetics,2000,155(2):945-959.

Please choose a citation manager

Content to export