Expression Patterns of Wheat Transcription Factor Gene TaNF-YB2; 1 and Its Genetic Effects on Plant Tolerance to Stresses of Drought and Salt

doi:10.7668/hbnxb.2015.02.001

Abstract

Abstract: NF-YB type transcription factor family plays critical roles in mediating plant tolerance to diverse abiotic stresses.The purpose of this study was to understand the expression patterns of TaNF-YB2; 1, a NF-YB transcription factor gene in wheat, and determine the function of this gene in regulating plant tolerance to aforementioned stresses.The expression patterns of TaNF-YB2; 1 were determined based on semiquantitative RT-PCR and transgenic tobacco plants overexpressing TaNF-YB2; 1 were generated by adopting the Agrobacterium-mediated genetic transformation approach.The results indicated that TaNF-YB2; 1 had a cDNA full length of 958 bp, encoded a 163 amino acid-polypeptide.Under the conditions of drought and salt stress, the transcripts of TaNF-YB2; 1 were drastically upregualted in comparison those under the condition of normal growth, suggested that it responds to above stressors.Under normal growth, the growth features and dry mass of the plants overexpressing TaNF-YB2; 1 were similar to wild type.However, the plants overexpressing TaNF-YB2; 1 exhibited significantly improved growth features and dry mass compared with the wild type plants under treatments of drought and salt.Therefore, the expression of TaNF-YB2; 1 was dramatically induced by stresses of drought and high salinity. TaNF-YB2; 1 acts as a critical regulator in mediating plant tolerance to drought and salt through its response to above stressors.

Key words: Wheat, Transcription factor gene TaNF-YB2, 1, Expression, Drought and Salt stress, Tolerance

CLC Number:

S512.03
Q78

CHEN Fang, ZHI Yi-ming, XIAO Kai. Expression Patterns of Wheat Transcription Factor Gene TaNF-YB2; 1 and Its Genetic Effects on Plant Tolerance to Stresses of Drought and Salt[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 1-5. doi: 10.7668/hbnxb.2015.02.001.

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