The Relationship of Carbon-nitrogen Metabolism Between Nitrogen Efficiency and Its Characteristics of Rape

doi:10.7668/hbnxb.2015.01.037

Abstract

Abstract: In order to clarify the relationship of carbon-nitrogen metabolism with nitrogen efficiency and it's influence on quality of Brassica napus.Under soil cultivation with normal nitrogen supply and nitrogen stress supply conditions, studied two rape varieties:low-yield rape variety(variety A)and high-yield rape variety(variety B), assayed the difference and change of their photosynthetic parameters, soluble sugars, free amino acid, nitrate content, nitrogen efficiency, harvest index and fatty acid composition.The result showed that in two nitrogen conditions photosynthetic parameters of variety B were higher than variety A on seeding stage, while trend found in flowering stage were opposite.As for soluble sugars the amount of variety B is larger than that of variety in stem and leaves at flowering stage and silique stage, the amount of soluble sugars in stem and leaf of rape showed decreasing tendency from silique stage, moreover, compared with bolting stage sugars content at silique and harvest stage was reduce 53% -80%, soluble sugars of stem and leaf in two varieties were lower in normal nitrogen conditions while than in nitrogen stress;the free amino acid of variety B of leaves at flowering stage, aboveground part at pod stage in two nitrogen conditions and leaves at bolting stage in nitrogen stress condition was lower than that of variety A, the adverse trend was found in two varieties in the other part and conditions.Free amino acids of the two varieties were decreased largely at pop stage.Amount of nitrate of rape in five stages, nitrogen efficiency, harvest index and quality of variety B were higher than that of variety A.Carbon-nitrogen metabolism has close influence on nitrogen efficiency, in favour of nitrogen accumulation and decreased largely at late stare, the variety had high carbon-nitrogen metabolism had high nitrogen efficiency and quality, variety B were superior to variety A, which has obvious breed goodness.

Key words: Brassica napus, Carbon-nitrogen metabolism, Nitrogen efficiency, Free amino acid

CLC Number:

YU Jia-ling, ZHANG Li, ZHANG Zhen-hua, SHU Jia-bin, SONG Hai-xing, GUAN Chun-yun. The Relationship of Carbon-nitrogen Metabolism Between Nitrogen Efficiency and Its Characteristics of Rape[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(1): 219-224. doi: 10.7668/hbnxb.2015.01.037.

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References

[1] Huppe H C,Turpin D H.Intergration of carbon and nitrogen metabolism in plant and algal cells[J].Ann Rev Physiol Plant Mol Riol,1994,45:577-607.
[2] Plaut Z,Lendzian K,Bassham J A.Nitrite reduction in reconstituted and whole spinach ehloroplasts during carbon dioxide reduction[J].Plant Physiology,1977,59(2):184-188.
[3] 宋建民,田纪春,赵世杰.植物光合碳和氮代谢之间的关系及其调节[J].植物生理学通讯,1998,34(3):230-238.
[4] De La Torre A,Delgado B,Lara C.Nitrate-Dependent O₂ evolution in intact leaves[J].Plant Physiol,1991,96:898-901.
[5] 张敏,陶诗顺,黄露,等.甘蓝型杂交油菜干物质积累、分配及其与产量关系研究[J].中国种业,2009(11):51-54.
[6] 刘浩荣,宋海星,刘代平,等.油菜茎叶可溶性糖与游离氨基酸含量的动态变化[J].西北农业学报,2007,16(1):123-126.
[7] Halford N G,Hey S,Jhurreea D,et al.Highly conserved protein kinases involved in the regulation of carbon and amino acid metabolism[J].Journal of Experimental Botany,2004,55(394):35-42.
[8] Good A G,Shrawat A K,Muench D G.Can less yield more? Is reducing nutrient input into the environment compatible with maintaining crop production[J].Trends in Plant Science,2004,9(12):597-605.
[9] 庞静,朱建国,刘刚.开放式空气CO₂浓度增高对水稻体内不同形态N素含量的影响[J].农业环境科学学报,2005,24(5):833-837.
[10] 鲍士旦.土壤农化分析[M].北京:中国农业出版社,1999.
[11] 邹琦.植物生理生化实验指导[M].北京:中国农业出版社,1995.
[12] 郑炳松.现代植物生理生化研究技术[M].北京:气象出版社,2006.
[13] Yanagisawa S,Akiyama A,Kisaka H,et al.Metabolic engineering with Dof1 transcription factor in plants:Improved nitrogen assimilation and growth under low-nitrogen conditions[J].Proceedings of the National Academy of Sciences of the United States of America,2004,101(20):7833-7838.
[14] Hikosaka K.Interspecific difference in the photosynthesis-nitrogen relationship:patterns,physiological causes,and ecological importance[J].Journal of Plant Research,2004,117(6):481-494.
[15] Daepp M N,Sberger J,Locher A.Nitrogen fertilization and developmenta1 stage alter the response of lolium perenne to elevated CO₂[J].New Physiologist,200l(150):347-358.
[16] 李燕婷,米国华,陈范骏,等.玉米幼苗地上部/根间氮的循环及其基因型差异[J].植物生理学报,2001,27(3):226-230.
[17] 云菲,刘国顺,史宏志.光氮互作对烟草气体交换和部分碳氮代谢酶活性及品质的影响[J].作物学报,2010,36(3):508-516.
[18] 赵宪凤,刘卫群,王树会.氮、磷、钾对烤烟碳氮代谢关键酶活性及其经济效益的影响[J].华北农学报,2012,27(4):181-185.
[19] Nakano H,Makino A,Mae T.The effect of elevated partial pressures of CO₂ on the relationship between photosynthetic capacity and N content in rice leaves[J].Plant physiology,1995,115:191-198.
[20] 郭庆法,王庆成,汪黎明.中国玉米栽培学[M].上海:上海科学技术出版社,2004:117-167.
[21] Olivera I C,Coruzzi G M.Carbon and amino acids reciprocally modulate the expression of glutamine synthase in Arabidopsis[J].Plant Physiology,1999(121):301-309.
[22] Wilson A,Pickett F B,Turner J C,et al.A dominant mutation in Arabidopsis confers resistance to auxin,ethylene and abscisci acid[J].Molecular and General Genetics,1990,222(2/3):377-383.
[23] Sinclair T R,De C T.Photosynthate and nitrogen requirements for seed production by various crops[J].Science,1975,189(4202):565-567.
[24] 宋海星,彭建伟,刘强,等.不同氮素生理效率油菜生育后期氮素再分配特性研究[J].中国农业科学,2008,41(6):1858-1864.

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