Mapping Quantitative Trait Loci Associated with Height and Tillers of Oryza rufipogon Griff.Dwarf Mutant and Genetic Analysis of Major Quantitative Locus

doi:10.7668/hbnxb.2014.05.002

Abstract

Abstract: In order to exploit and isolate more genes controlling plant height and effective tillers,and applies these genes into breeding practice.In this paper,an F₂ isolated population was constructed derived from a cross between a multi-tillering dwarf wild rice mutant and minor-tillering tall Nantehao variety;plant height and effective tillers of the F₂ population were investigated and their genotypes were tested,and QTLs linkage genetic analysis was conducted by using IciMapping V3.0 soft.Plant height and effective tillers of the two parents were all quantitative traits controlled by multi-genes and had obvious positive relation.194 pairs of molecular markers were identified to have polymorphism between the parents by screening 345 pairs of molecular markers even distributing on 12 chromosomes in rice and the polymorphic ratio was of 56.23%.In the F₂ population,33 height QTLs and 19 tillers QTLs were obtained using linkage analysis of 122 pairs of molecular markers. One major QTL controlling height was identified and located in the interval markers of RM302 and RM104 on chromosome 1.The major QTL explained 71.72% phenotypic variation.In our studies,a major QTL controlling plant height was obtained which was closely linkage with molecular markers of RM302 and RM104.It had very strong dwarfed ability.Our results would provide a theoretical basis for cloning dwarf genes and molecular breeding of constructing ideal plant shape.

Key words: Rice, Oryza rufipogon, Height, Tillers, QTLs, Genetic analysis

CLC Number:

WANG Lan, LI Zhi, ZHENG Xing-mei, CAI Ying-qin, LUO Min, NIE Yi-yong. Mapping Quantitative Trait Loci Associated with Height and Tillers of Oryza rufipogon Griff.Dwarf Mutant and Genetic Analysis of Major Quantitative Locus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(5): 5-9. doi: 10.7668/hbnxb.2014.05.002.

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