Abstract: The aims of this study are to clone the catalytic subunit gene of rice protein phosphatase 6 (OsPP6C),construct the over-expressing vector of this gene and conduct the bioinformatics analysis. Using the RT-PCR technique,the full length of OsPP6C gene was amplified from the complementary DNA (cDNA)of rice Zhong-hua 11 variety,and it was fused with GUS to form pBI121-OsPP6C-GUS expression vector.Through bioinformatics methods,the physiochemical characteristics of OsPP6C protein,the homology among Arabidopsis PP6Cs(i.e.At-FyPPs)and OsPP6C,and the phylogenetic relationship among different species were analyzed;in addition,the cis-acting elements of the OsPP6C promoter were analyzed. The results show that the transcript sequence of this gene contains an open reading frame of 912 base pairs, this gene encodes a peptide of 303 amino acids, the molecular weight and isoelectric point of this protein is approximately 3.47 kDa and 5.13,respectively;the OsPP6C protein The aims of this study are to clone the catalytic subunit gene of rice protein phosphatase 6 (OsPP6C),construct the over-expressing vector of this gene and conduct the bioinformatics analysis. Using the RT-PCR technique,the full length of OsPP6C gene was amplified from the complementary DNA (cDNA)of rice Zhong-hua 11 variety,and it was fused with GUS to form pBI121-OsPP6C-GUS expression vector.Through bioinformatics methods,the physiochemical characteristics of OsPP6C protein,the homology among Arabidopsis PP6Cs(i.e.At-FyPPs)and OsPP6C,and the phylogenetic relationship among different species were analyzed;in addition,the cis-acting elements of the OsPP6C promoter were analyzed. The results show that the transcript sequence of this gene contains an open reading frame of 912 base pairs, this gene encodes a peptide of 303 amino acids, the molecular weight and isoelectric point of this protein is approximately 3.47 kDa and 5.13,respectively;the OsPP6C protein has the hydrophobic property,but there is no signal peptide in this protein and it belongs to the non-secretory pro-teins.The amino acid identity of OsPP6C with AtFyPP1 and AtFyPP3 was 93.73%and 93.40%,respectively;the phylogenetic tree shows that OsPP6C has the closest genetic relationship with wheat TaPP 6C.The cis-acting element analysis in the promoter of this gene shows that besides the TATA box and CAAT box,the promoter also contains multiple light-responsive elements,various responsive elements in relation to hormones such as ABA,ethylene,aux-in,methyl jasmonate ( MeJA ) and gibberellic acid, and the regulatory elements involved in various abiotic stresses such as low temperature,heat stress and drought. This paper will lay a solid foundation for further studying expres-sion characteristics and functions of OsPP6 C gene in rice.

Key words: Oryza sativa, Protein phosphatase 6, Vector construction for overexpression, Bioinformatics analysis

CLC Number: 

• S511