Porcine Circovirus like Virus P1 Infection and Its Molecular Characteristics

doi:10.7668/hbnxb.2013.05.006

Abstract

Abstract: Porcine circovirus like virus P1 is a new pathogen emerging in porcine and causing postweaning mul-tisystemic wasting syndrome ( PMWS)-like signs.P1 has the smallest genome up to now. To investigate the preva-lence and molecular characteristics of P1, complete viral DNA genome sequences were amplified by PCR, cloned and sequenced. Bioinformatics was employed to analyze the nucleotide sequence and phylogeny. The results of this study indicated that the prevalence of P1 in the pig farms were quite high,the nucleotide sequence similarity of P1 was 99. 1%to 100%,and it could be classified in two sublineage;Meanwhile,distribution of P1 in samples were al-so detected by immunohistochemistry.

Key words: Porcine circovirus like virus P1, PCR, Immunohistochemistry, Molecular characteristics

CLC Number:

S852.65

WEN Li-bin, HE Kong-wang, WANG Feng-zhi, YU Zheng-yu, MAO Ai-hua, XIE Jian-ping. Porcine Circovirus like Virus P1 Infection and Its Molecular Characteristics[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(5): 34-37. doi: 10.7668/hbnxb.2013.05.006.

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http://www.hbnxb.net/EN/Y2013/V28/I5/34

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