Mapping QTL Associated with Grain Number of Rice

doi:10.3969/j.issn.1000-7091.2013.03.004

Abstract

Abstract: A F₂ population from Nipponhare and 80801 ( a bid-ear variety with more spikelets and gains) was used in the study，and 11 QTLs associated with grain number， spike length and grain density were detected，which distributed on the chromosome 1，2，5，7，9 respectively． The LOD value was 2.03-31.88，and the contribution rate of phenotype ranged from 0.21% to 68.20%．4 QTLs were associated with grain number，distributing on the chromosome 1，2，7 and 9，respectively，with the LOD value 2.11-4.00 and the genotype contribution rate 0.21%-25.06%． 4 QTLs about spikelet length were mapped on chromosome 1，2，5，9 the LOD value being 2.03-31.88and the genotype contribution 0.28%-61.19%． Among them，qPL9-1 had the LOD value of 31.88 and contribution rate of 68.19%，which should be a major QTL． Besides，3 QTLs about grain density were located on chromosome 1，2，9 the LOD value being 2.15-2.64 and the phenotype contribution rate being 0.43%-6.91.

Key words: Rice, Spike length, Grain density, Grain number, QTL

CLC Number:

Pan Yinghua, Yu Jianping, Zhang Qiang, Xiong Haiyan, Zhu Rucai, Li Zichao. Mapping QTL Associated with Grain Number of Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 19-27. doi: 10.3969/j.issn.1000-7091.2013.03.004.

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References

[1] Ashikari M，Sakakibara H，Lin S Y，et al．Cytokinin Oxidase Regulates Rice Grain Production[J]．Science， 2005，309:741-745.
[2] Xue W，Xing Y Z，Weng X Y，et al． Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice[J]，Nature Genetics， 2008， 40(6):761-767.
[3] Wen H Y，Peng W，Hua X C， et al． A Major QTL，Ghd8，Plays Pleiotropic Roles in Regulating Grain Productivity，Plant Height， and Heading Date in Rice[J]．Mol Plant，2011，4(2):319-330.
[4] Li M，Tang D，Wang K J， et al． Mutations in the F-boxgene LARGER PANICLE improve the panicle architecture and enhance the grain yield in rice[J]． Plant Biotechnology (9):1002-1013.
[5] Huang X H，Qian Q，Liu Z B， et al． Natural variation at the DEP1 locus enhances grain yield in rice[J]． NatureGenetics， 2009， 41(4):494-497.
[6] Yuki A，Keiko M，Tsuyu A，et al． The SMALL AND ROUND SEED1 (SRS1/DEP2) gene is involved in the regulation of seed size in rice[J]． Genes and Genetic Systems， 2010，85( 5):327-339．
[7] Qiao Y L，Piao R H，Shi J H， et al． Fine mapping and candidate gene analysis of dense and erect panicle 3，DEP3，which confers high grain yield in rice ( Oryza sativa L.) [J]． Theoretical and Applied Genetics， 2011， 122 (7):1439-1449
[8] Piao R H， Jiang W Z，Ham T H， et al． Map-based cloning of the ERECT PANICLE 3 gene in rice[J]． Theoretical and Applied Genetics， 2009， 119(8):1497-1506．
[9] FumioT S，Yasushi K，Hiroshi K，et al． A loss-of-function mutation of rice DENSE PANICLE 1 causes semi-dwarfness and slightly increased number of spikelets [J]．Breeding Science， 2011， 61(1):17-25.
[10] Li S B，Qian Q，Fu Z M， et al． Short panicle1 encodes a putative PTR family transporter and determines rice panicle size[J]．The Plant Journal，2009，58 (4):592-605.
[11] 韩龙植，魏兴华．水稻种质资源描述规范和数据标准[M]．北京: 中国农业出版社， 2006．
[12] Murray M G，Thompson W F． Rapid isolation of high molecular weight plant DNA[J]．Nucleic Acids Research，1980，8(19):4321-4326．
[13] McCouch S R，Kochert G，Yu Z H， et al． Molecular mapping of rice chromosomes[J]． Theoretical and Applied Genetics,1988,76:815-829
[14] Panaud O，Chen X，McCouch S R． Development of microsatellite markers and characterization of simple sequence length polymorphism ( SSLP) in rice ( Oryza sativa L．) [J]．Molecular and General Genetics， 1996， 252(5):597-607.
[15] 王建康．数量性状基因的完备区间作图方法[J]．作物学报，2009，35(2):239-245．
[16] McCouch S R，Cho Y G，Yano M， et al． Report on QTL nomenclature[J]． Rice Genet Newslett， 1997(14):11-13．
[17] Stuber C W，Lincoln S E，Wolff D W， et al． Identification of genetic factors contributing to heterosis in a hybrid from two elite maize inbred lines using molecular markers[J]． Genetics， 1992， 132(6):823-839．
[18] 刘仁虎，孟金陵． Mapdraw，在Excel 中绘制遗传连锁图的宏[J]．遗传，2003，25(3):317-321.
[19] 邢永忠，谈移芳，徐才国，等．利用水稻重组自交系群体定位谷粒外观性状的数量性状基因[J]．植物学报，2001，43(8) 840-845．

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