Screening， Identification and Sporulation Conditions Optimization of NX-11 Strain Having the Ability of Solubilizing Phosphorus and Potassium

doi:10.3969/j.issn.1000-7091.2013.02.036

Abstract

Abstract: Bacteria having the efficient activity on solubilizing phosphate and potassium have been isolated for its utilization as a biological fertilizer. The most efficient strain named NX-11 was chosen for further study. NX-11 was identified as Bacillus subtilis after a series of physiological and biochemical experiments,morphological observa-tion and 16S rRNAgene sequence analysis. The solubilization ability of NX-11 was tested in the condition of pure cul-ture. The results showed that the optimal medial components were composed of 5.0% starch,1.5% soybean cake powder,0.07%mgSO4,0.05% NaCl. The optimal culture conditions were at temperature 37℃ with initial pH 8. 0 and incubation time 20 h,inoculum's volume 40mL/L,medium volume 30mL in 250mL flask and 200 r/min.

Key words: Bacillus subtilis, Phosphate solubilization, Potassium solubilization, Orthogonal test

CLC Number:

S432. 4

MA Rui-yang, ZHANG Ai-min, HUI Xiao-shuang, DAI Meng, WANG Wen, ZHU Bao-cheng. Screening， Identification and Sporulation Conditions Optimization of NX-11 Strain Having the Ability of Solubilizing Phosphorus and Potassium[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(2): 202-208. doi: 10.3969/j.issn.1000-7091.2013.02.036.

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