Abstract: Conidiation-deficiency transformant BCG-183 was obstained from more than 400 ATMT transformants.The genomic DNA of wild type and BCG-183 were extracted by CTAB method,and southern blot showed that one copy of T-DNA had inserted into the mutant's DNA.By analysing the phenotype of the mutant,we found that mutant's hyphae were more dense than the wild type,while its colony was white.The mutant didn't produce conidium.And the cell-wall-degrading enzyme activities were stronger than that of the wild type′s.T-DNA insertion site flanking sequence was amplified by TAIL-PCR.It showed that the T-DNA had inserted into the putative protein coding genes(BC1G_07455)3′termination,which localized in the Supercontig 42(Supercontig42)of Botrytis cinerea genome.RT-PCR results showed that BC1G_07455 didn′t express in the mutant.These results indicated that BC1G_07455 may involve in the conidiation of Botrytis cinerea.

Key words: Botrytis cinerea, Conidiation, TAIL-PCR, RT-PCR

CLC Number: 

• S36.631