Anti-ampicillin Monoclonal Antibodies and Their Cross-reactivity to Penicillin Antibiotics

doi:10.7668/hbnxb.2011.03.013

Abstract

Abstract: Ampicillin(Amp) was coupled to carrier proteins of bovine serum albumin(BSA) and ovalbumin(OVA) to synthetize immunogen BSA-Amp and detection antigen OVA-Amp.Four hybridoma cell lines secreting monoclonal antibodies(mAbs) specific for Amp were established by immunizing BALB/c mice with BSA-Amp using hybridoma technology.The antibody titers of mAb ascites(1G7,2A11,2D10 and 3F6) were determined as 1∶2.56×10⁷,1∶1.28×10⁷,1∶5.12×10⁶ and 1∶5.12×10⁶ in ELISA,and their half maximal inhibitory concentration(IC₅₀) of Amp were 10.22,3.58,4.03,4.95 ng/mL in the inhibitive ELISA,respectively.The Amp mAbs showed significant cross-reactivity to the penicillin antibiotics of carbenicillin(1G7,2A11,2D10 and 3F6),penicillin G(1G7 and 3F6),and amoxicillin(1G7),whereas no obvious cross-reactivity to dicloxacillin or cloxacillin was found.

Key words: Ampicillin, Monoclonal antibody, Cross-reactivity

CLC Number:

LI Qing-mei, LIU Qing-tang, WANG Yin-biao, CHAI Shu-jun, WANG Zi-liang, GUO Jun-qing, ZHI Ai-min, MENG Hong-li, ZHANG Gai-ping. Anti-ampicillin Monoclonal Antibodies and Their Cross-reactivity to Penicillin Antibiotics[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(3): 60-63. doi: 10.7668/hbnxb.2011.03.013.

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