A SSH Library Constructed at Jointing Stage Induced by Water Stress in Wheat

doi:10.7668/hbnxb.2011.01.008

Abstract

Abstract: The drought is the main factor that caused drop in production. Some related genes expressed when theplant suffered water stress. A cDNA library induced by water stress was constructed by the method of suppression sub-tractive hybridization (SSH).Based on the sepuencing data we obtained a total of 2046 good quality ESTs. Therewere 961 contigs based on EST cluster result by DNAStar software. Through EST similarity analysis we got functionUnienes belonging to 268 categories that involved in 12 kinds of metabolism pathway and showed direct or indirectcell protection response to dehydration.

Key words: Wheat, Jointing stake, Gene expression, Water stress, EST

CLC Number:

S512.103.2

SUN Chang-qing, SHI Jun-feng, GUO Zhi-li. A SSH Library Constructed at Jointing Stage Induced by Water Stress in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(1): 37-40. doi: 10.7668/hbnxb.2011.01.008.

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http://www.hbnxb.net/EN/Y2011/V26/I1/37

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