Effects of P1 Infection on Transcriptional Levels of TLRs in Peripheral Blood Mononuclear Cells of Piglets

doi:10.7668/hbnxb.2010.06.002

Abstract

Abstract: The transcriptional levels of Toll-like receptors(TLRs)in peripheral blood mononuclear cells(PB-MCs)from piglets were detected by real-time RT·PCR.The results showed that mRNA levels of most TLRs, except TLR2 and TLRl0, were down·regulated at 3 days post-inoculation(DPI).Whereas the expression levels of TLR2 at 24 and 40 DPI were significantly higher in piglets with P1 infection than that in healthy controls(P<0.05)；TLR9 mRNA expression at 3 1 and 40 DPI showed an upward trend(P<0.1).These results suggest that the inflammation reac·tion mediated by TLR2 and TLR9 might play an important role in recognizing P1 and participating in the pathogenesis of P1.

Key words: Porcine circovirus—like agent, Toll—like receptors(TLRs), Peripheral blood mononuclear cells (PBMCs), Real.time PCR

CLC Number:

S855.3

WEN Li-bin, HE Kong-wang, YANG Han-chun, GUO Rong-li, ZHONG Shu-lin, YU Zheng-yu, MAO Ai-hua. Effects of P1 Infection on Transcriptional Levels of TLRs in Peripheral Blood Mononuclear Cells of Piglets[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(6): 9-13. doi: 10.7668/hbnxb.2010.06.002.

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References

[1] Hashimoto C，Hudson K L，Anderson K V.The Toll gene of Drosophila，required for dorsoal—ventral embryonic po—larity，appears to encode a transmembrane protein[J].Cell，1988，52(2)：269—279.
[2] Shimosato T，Kitazawa H，Katoh S，et a1.Swine Toll.1ike receptor 9 recognizes CpG motifs of human cell stimulant [J].Biochim Biophys Acta，2003，1627(1)：56—61.
[3] Shinkai H，Muneta Y，Suzuki K，et a1.Porcine Toll.1ike receptor 1，6，and 10 genes：complete sequencing of genomic region and expression analysis[J].Mol Immun01. 2006，43(9)：1474—1480.
[4] Shinkai H，Tanaka M，Morozumi T，et a1.Biased distribu.tion of single nucleotide polymorphisms(SNPs)in porcine Toll—like receptor 1(TLRl)，TLR2，TLR4，TLR5，and TLR6 genes[J].Immunogenetics，2006，58(4)：324—330.
[5] 金伯泉.固有免疫中模式识别受体及其信号转导——当代免疫学中最伟大的发现之一[J].细胞与分子免疫学杂志，2006，22：1—3.
[6] Akira S，Uematsn s，Takeuchi O.Pathogen recognition and innate immunity[J].Cell，2006，124(4)：783—801.
[7] 温立斌，何孔旺，杨汉春.一株类猪圆环病毒2型因子Pl的全基因组序列测定与分析[J].中国农业科学，2010，43(2)：411—416.
[8] 温立斌，何孔旺，杨汉春.P1因子分子克隆的体外感染性分析[J].畜牧兽医学报，2008，39：941—944.
[9] 温立斌，何孔旺，杨汉春，等.类猪圆环病毒因子P1感染对猪红细胞的影响[j].内蒙古农业科技，2009(2)：46—48.
[10] 温立斌，何孔旺，杨汉春，等.TUNEL法检测类猪圆环病毒2型因子P1诱导猪免疫器官细胞凋亡研究[J].华北农学报，2008，23(增刊)：88—91.
[11] Trinchieri G.Sher A.Cooperation of Toll—like receptor signals in innate immune defence[J].Nat Rev Immu-nol，2007，7(3)：179—190.
[12] Miyake K.Innate immune sensing of pathogens and danger signals by cell surface Toll·like receptors[J].Semin Immunol。2007，19(1)：3—10.
[13] Hayashi F，Smith K D，Ozinsky A，et a1.The innate immune response to bacterial flagellin is mediated by Toll—like receptor 5[J].Nature，2001，410(6832)：1099一1103.
[14] Lund J M，Alexopoulou L，Sato A，et a1.Recognition of
[15] single-stranded RNA viruses by Toll—like receptor 7[J].2004，10l(15)：5598—5603.
[15] Beignon A S，McKenna K，Skoberne M，et a1.Endoey tosis of HIV-1 activates plasmacytoid dendritic cells via Toll·like receptor·viral RNA interactions[J].J Clin In—vest，2005，115(11)：3265—3275.
[16] Zhang Y L，Guo Y J，Bin Li，et a1.Hepatitis C virussingle·stranded RNA induces innate immunity via Toll—-like receptor 7[J].J Hepatol，2009，51(1)：29—38.
[17] Akira S，Hemmi H.Recognition of pathogen·associated molecular patterns by TLR family[J].Immunol lett，2003，85(2)：85—95.
[18] Heggelund L，Moiler F，Lien E。et a1.Increased expression of toll—like receptor 2 on monoeytes in HIV in feetion：possible roles in inflammation and viral replication[J].Clin Infect Dis，2004，39(2)：264—269.
[19] Hllrter L，Mica L，Stocker R，et a1.Increased expression of toll-like receptor-2 and-4 on leukocytes from pa-tients with sepsis[J].Shock，2004，22(5)：403—409.

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